Adding some utilities to test unmapped reads
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@887 348d0f76-0448-11de-a6fe-93d51630548a
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@ -3,8 +3,10 @@ package org.broadinstitute.sting.utils.sam;
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import net.sf.samtools.*;
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import net.sf.samtools.*;
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import java.io.File;
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import java.io.File;
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import java.util.ArrayList;
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import java.util.*;
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import java.util.List;
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import org.broadinstitute.sting.gatk.iterators.StingSAMIterator;
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import org.broadinstitute.sting.gatk.Reads;
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/**
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/**
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*
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*
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@ -33,8 +35,11 @@ import java.util.List;
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* A collection of utilities for making sam and bam files. Mostly these are for creating sam and bam files for testing purposes.
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* A collection of utilities for making sam and bam files. Mostly these are for creating sam and bam files for testing purposes.
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*/
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*/
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public class ArtificialSamUtils {
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public class ArtificialSamUtils {
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public static final int DEFAULT_READ_LENGTH = 50;
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/**
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/**
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* create an artificial sam file
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* create an artificial sam file
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*
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* @param filename the filename to write to
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* @param filename the filename to write to
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* @param numberOfChromosomes the number of chromosomes
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* @param numberOfChromosomes the number of chromosomes
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* @param startingChromosome where to start counting
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* @param startingChromosome where to start counting
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@ -58,6 +63,7 @@ public class ArtificialSamUtils {
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/**
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/**
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* create an artificial sam file
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* create an artificial sam file
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*
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* @param filename the filename to write to
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* @param filename the filename to write to
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* @param numberOfChromosomes the number of chromosomes
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* @param numberOfChromosomes the number of chromosomes
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* @param startingChromosome where to start counting
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* @param startingChromosome where to start counting
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@ -81,6 +87,7 @@ public class ArtificialSamUtils {
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/**
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/**
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* Creates an artificial sam header, matching the parameters, chromosomes which will be labeled chr1, chr2, etc
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* Creates an artificial sam header, matching the parameters, chromosomes which will be labeled chr1, chr2, etc
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*
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* @param numberOfChromosomes the number of chromosomes to create
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* @param numberOfChromosomes the number of chromosomes to create
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* @param startingChromosome the starting number for the chromosome (most likely set to 1)
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* @param startingChromosome the starting number for the chromosome (most likely set to 1)
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* @param chromosomeSize the length of each chromosome
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* @param chromosomeSize the length of each chromosome
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@ -92,7 +99,7 @@ public class ArtificialSamUtils {
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// make up some sequence records
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// make up some sequence records
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for (int x = startingChromosome; x < startingChromosome + numberOfChromosomes; x++) {
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for (int x = startingChromosome; x < startingChromosome + numberOfChromosomes; x++) {
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SAMSequenceRecord rec = new SAMSequenceRecord("chr" + (x), chromosomeSize /* size */);
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SAMSequenceRecord rec = new SAMSequenceRecord("chr" + (x), chromosomeSize /* size */);
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rec.setSequenceLength(1000);
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rec.setSequenceLength(chromosomeSize);
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dict.addSequence(rec);
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dict.addSequence(rec);
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}
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}
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header.setSequenceDictionary(dict);
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header.setSequenceDictionary(dict);
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@ -101,6 +108,7 @@ public class ArtificialSamUtils {
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/**
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/**
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* Create an artificial read based on the parameters. The cigar string will be *M, where * is the length of the read
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* Create an artificial read based on the parameters. The cigar string will be *M, where * is the length of the read
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*
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* @param header the SAM header to associate the read with
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* @param header the SAM header to associate the read with
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* @param name the name of the read
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* @param name the name of the read
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* @param refIndex the reference index, i.e. what chromosome to associate it with
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* @param refIndex the reference index, i.e. what chromosome to associate it with
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@ -120,4 +128,98 @@ public class ArtificialSamUtils {
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return record;
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return record;
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}
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}
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/**
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* create an iterator containing the specified read piles
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* @param startingChr the chromosome (reference ID) to start from
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* @param endingChr the id to end with
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* @param readCount the number of reads per chromosome
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* @return StingSAMIterator representing the specified amount of fake data
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*/
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public static StingSAMIterator unmappedReadIterator(int startingChr, int endingChr, int readCount ) {
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SAMFileHeader header = createArtificialSamHeader((endingChr - startingChr) + 1, startingChr, readCount + DEFAULT_READ_LENGTH);
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Map<Integer, Integer> map = new HashMap<Integer, Integer>();
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for (int x = startingChr; x < endingChr; x++) {
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map.put(x,readCount);
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}
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return new ArtificialSAMIterator(startingChr, endingChr, readCount,header);
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}
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}
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/**
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* this fake iterator allows us to look at how specific piles of reads are handled
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*/
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class ArtificialSAMIterator implements StingSAMIterator {
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private int currentChromo = 0;
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private int currentRead = 0;
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private int readCount = 0;
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private boolean done = false;
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// the next record
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private SAMRecord next = null;
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SAMFileHeader header = null;
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// the passed in parameters
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final int sChr;
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final int eChr;
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final int rCount;
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/**
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* create the fake iterator, given the mapping of chromosomes and read counts
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* @param startingChr the starting chromosome
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* @param endingChr the ending chromosome
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* @param readCount the number of reads in each chromosome
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* @param header the associated header
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*/
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ArtificialSAMIterator(int startingChr, int endingChr, int readCount, SAMFileHeader header) {
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sChr = startingChr;
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eChr = endingChr;
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rCount = readCount;
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this.header = header;
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this.currentChromo = startingChr;
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}
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public Reads getSourceInfo() {
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throw new UnsupportedOperationException("We don't support this");
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}
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public void close() {
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// done
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currentChromo = Integer.MAX_VALUE;
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}
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public boolean hasNext() {
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if (currentRead >= rCount) {
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currentChromo++;
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currentRead = 0;
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}
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if (currentChromo >= eChr) {
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return false;
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}
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int alignment = 0;
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if (currentChromo >= 0) {
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alignment = currentRead;
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} else {
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alignment = 0;
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}
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this.next = ArtificialSamUtils.createArtificialRead(this.header,String.valueOf(readCount), currentChromo, alignment, 50);
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currentRead++;
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return true;
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}
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public SAMRecord next() {
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return next;
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}
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public void remove() {
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throw new UnsupportedOperationException("You've tried to remove on a StingSAMIterator (unsupported), not to mention that this is a fake iterator.");
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}
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public Iterator<SAMRecord> iterator() {
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return this;
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}
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}
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}
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