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Changed ReadBackedPhasing to be a RodWalker (corrected to By(READS)) 

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4120 348d0f76-0448-11de-a6fe-93d51630548a
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fromer 2010-08-25 20:53:04 +00:00
parent a7af605d95
commit 39da567d48
1 changed files with 12 additions and 10 deletions
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22
java/src/org/broadinstitute/sting/playground/gatk/walkers/ReadBackedPhasingWalker.java
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@ -49,11 +49,11 @@ import static org.broadinstitute.sting.utils.vcf.VCFUtils.getVCFHeadersFromRods;
/**
* Walks along all loci, caching a user-defined window of VariantContext sites, and then finishes phasing them when they go out of range (using downstream reads).
* Use '-BTI variant' to only stop at positions in the VCF file bound to 'variant'.
* Walks along all variant ROD loci, caching a user-defined window of VariantContext sites, and then finishes phasing them when they go out of range (using upstream and downstream reads).
*/
@Allows(value = {DataSource.READS, DataSource.REFERENCE})
@Requires(value = {DataSource.READS, DataSource.REFERENCE}, referenceMetaData = @RMD(name = "variant", type = ReferenceOrderedDatum.class))
@By(DataSource.READS)
@ReadFilters( {ZeroMappingQualityReadFilter.class} ) // Filter out all reads with zero mapping quality
@ -340,7 +340,7 @@ public class ReadBackedPhasingWalker extends RodWalker<PhasingStatsAndOutput, Ph
}
if (statsWriter != null)
statsWriter.addStat(samp, VariantContextUtils.getLocation(vc), distance(prevVc, vc), pr.phaseQuality);
statsWriter.addStat(samp, VariantContextUtils.getLocation(vc), distance(prevVc, vc), pr.phaseQuality, pr.numReads);
PhaseCounts sampPhaseCounts = samplePhaseStats.get(samp);
if (sampPhaseCounts == null) {
@ -378,7 +378,7 @@ public class ReadBackedPhasingWalker extends RodWalker<PhasingStatsAndOutput, Ph
allPositions.add(readBases);
}
HashMap<String, Read> allReads = convertReadBasesAtPositionToReads(allPositions);
logger.debug("Number of reads at sites: " + allReads.size());
logger.debug("Number of TOTAL reads [including those covering only 1 position] at sites: " + allReads.size());
int numUsedReads = 0;
// Update the phasing table based on each of the sub-reads for this sample:
@ -400,7 +400,7 @@ public class ReadBackedPhasingWalker extends RodWalker<PhasingStatsAndOutput, Ph
}
}
logger.debug("\nPhasing table [AFTER CALCULATION]:\n" + sampleHaps + "\n");
logger.debug("numUsedReads = " + numUsedReads);
logger.debug("numUsedReads [covering > 1 position in the haplotype] = " + numUsedReads);
// Marginalize each haplotype to its first 2 positions:
sampleHaps = HaplotypeTableCreator.marginalizeTable(sampleHaps);
@ -423,7 +423,7 @@ public class ReadBackedPhasingWalker extends RodWalker<PhasingStatsAndOutput, Ph
logger.debug("MAX hap:\t" + maxEntry.getHaplotypeClass() + "\tposteriorProb:\t" + posteriorProb + "\tphaseQuality:\t" + phaseQuality);
return new PhaseResult(maxEntry.getHaplotypeClass().getRepresentative(), phaseQuality);
return new PhaseResult(maxEntry.getHaplotypeClass().getRepresentative(), phaseQuality, numUsedReads);
}
/*
@ -922,10 +922,12 @@ public class ReadBackedPhasingWalker extends RodWalker<PhasingStatsAndOutput, Ph
private static class PhaseResult {
public Haplotype haplotype;
public double phaseQuality;
public int numReads;
public PhaseResult(Haplotype haplotype, double phaseQuality) {
public PhaseResult(Haplotype haplotype, double phaseQuality, int numReads) {
this.haplotype = haplotype;
this.phaseQuality = phaseQuality;
this.numReads = numReads;
}
}
}
@ -1257,10 +1259,10 @@ class PhasingQualityStatsWriter {
this.variantStatsFilePrefix = variantStatsFilePrefix;
}
public void addStat(String sample, GenomeLoc locus, int distanceFromPrevious, double phasingQuality) {
public void addStat(String sample, GenomeLoc locus, int distanceFromPrevious, double phasingQuality, int numReads) {
BufferedWriter sampWriter = sampleToStatsWriter.get(sample);
if (sampWriter == null) {
String fileName = variantStatsFilePrefix + "." + sample + ".locus_distance_PQ.txt";
String fileName = variantStatsFilePrefix + "." + sample + ".locus_distance_PQ_numReads.txt";
FileOutputStream output;
try {
@ -1272,7 +1274,7 @@ class PhasingQualityStatsWriter {
sampleToStatsWriter.put(sample, sampWriter);
}
try {
sampWriter.write(locus + "\t" + distanceFromPrevious + "\t" + phasingQuality + "\n");
sampWriter.write(locus + "\t" + distanceFromPrevious + "\t" + phasingQuality + "\t" + numReads + "\n");
sampWriter.flush();
} catch (IOException e) {
throw new RuntimeException("Unable to write to per-sample phasing quality stats file", e);