Added the new by reads traversal, still needs to be sewn into the micromanager code.
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@551 348d0f76-0448-11de-a6fe-93d51630548a
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package org.broadinstitute.sting.gatk.traversals;
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import net.sf.samtools.SAMRecord;
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import org.apache.log4j.Logger;
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import org.broadinstitute.sting.gatk.LocusContext;
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import org.broadinstitute.sting.gatk.dataSources.providers.LocusContextProvider;
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import org.broadinstitute.sting.gatk.dataSources.shards.Shard;
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import org.broadinstitute.sting.gatk.iterators.BoundedReadIterator;
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import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
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import org.broadinstitute.sting.gatk.refdata.ReferenceOrderedData;
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import org.broadinstitute.sting.gatk.refdata.ReferenceOrderedDatum;
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import org.broadinstitute.sting.gatk.walkers.ReadWalker;
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import org.broadinstitute.sting.gatk.walkers.Walker;
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import org.broadinstitute.sting.utils.GenomeLoc;
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import java.io.File;
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import java.util.List;
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/**
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*
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* User: aaron
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* Date: Apr 24, 2009
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* Time: 10:35:22 AM
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*
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* The Broad Institute
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* SOFTWARE COPYRIGHT NOTICE AGREEMENT
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* This software and its documentation are copyright 2009 by the
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* Broad Institute/Massachusetts Institute of Technology. All rights are reserved.
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*
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* This software is supplied without any warranty or guaranteed support whatsoever. Neither
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* the Broad Institute nor MIT can be responsible for its use, misuse, or functionality.
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*
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*/
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/**
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* @author aaron
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* @version 1.0
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* @date Apr 24, 2009
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* <p/>
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* Class TraverseReads
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* <p/>
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* This class handles traversing by reads in the new shardable style
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*/
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public class TraverseReads extends TraversalEngine {
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/** our log, which we want to capture anything from this class */
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protected static Logger logger = Logger.getLogger(TraverseReads.class);
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/**
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* Creates a new, uninitialized TraversalEngine
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*
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* @param reads SAM/BAM file of reads
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* @param ref Reference file in FASTA format, assumes a .dict file is also available
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* @param rods Array of reference ordered data sets
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*/
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public TraverseReads(List<File> reads, File ref, List<ReferenceOrderedData<? extends ReferenceOrderedDatum>> rods) {
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super(reads, ref, rods);
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}
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/**
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* Traverse by reads, given the data and the walker
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* @param walker the walker to execute over
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* @param shard the shard of data to feed the walker
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* @param locusProvider the factory for loci
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* @param sum of type T, the return from the walker
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* @param <M> the generic type
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* @param <T> the return type of the reduce function
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* @return
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*/
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public <M, T> T traverse(Walker<M, T> walker,
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Shard shard,
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LocusContextProvider locusProvider,
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BoundedReadIterator iter,
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T sum) {
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logger.debug(String.format("TraverseReads.traverse Genomic interval is %s", shard.getGenomeLoc()));
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if (!(walker instanceof ReadWalker))
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throw new IllegalArgumentException("Walker isn't a read walker!");
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ReadWalker<M, T> readWalker = (ReadWalker<M, T>) walker;
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GenomeLoc loc = shard.getGenomeLoc();
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// while we still have more reads
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for (SAMRecord read: iter) {
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// get the genome loc from the read
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GenomeLoc site = new GenomeLoc(read);
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// update the number of reads we've seen
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TraversalStatistics.nRecords++;
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// Iterate forward to get all reference ordered data covering this locus
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final RefMetaDataTracker tracker = getReferenceOrderedDataAtLocus(site);
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//ReferenceIterator refSite = referenceProvider.getReferenceSequence(site);
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LocusContext locus = locusProvider.getLocusContext(site);
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//locus.setReferenceContig(refSite.getCurrentContig());
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if (DOWNSAMPLE_BY_COVERAGE)
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locus.downsampleToCoverage(downsamplingCoverage);
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final boolean keepMeP = readWalker.filter(locus, read);
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if (keepMeP) {
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M x = readWalker.map(locus, read);
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sum = readWalker.reduce(x, sum);
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}
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printProgress("loci", locus.getLocation());
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}
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return sum;
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}
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}
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