diff --git a/scala/qscript/oneoffs/carneiro/downsampling.scala b/scala/qscript/oneoffs/carneiro/downsampling.scala
new file mode 100644
index 000000000..fcb0a8dc9
--- /dev/null
+++ b/scala/qscript/oneoffs/carneiro/downsampling.scala
@@ -0,0 +1,133 @@
+package oneoffs.carneiro
+
+import org.broadinstitute.sting.queue.QScript
+import org.broadinstitute.sting.queue.extensions.gatk._
+import scala.io.Source._
+/**
+ * Created by IntelliJ IDEA.
+ * User: carneiro
+ * Date: 3/17/11
+ * Time: 11:29 AM
+ * To change this template use File | Settings | File Templates.
+ */
+
+
+class downsampling extends QScript {
+
+  @Input(doc="path to GenomeAnalysisTK.jar", shortName="gatk", required=true)
+  var GATKjar: File = _
+
+  @Input(doc="input BAM file - or list of BAM files", shortName="i", required=true)
+  var input: File = _
+
+  @Input(doc="target intervals", shortName="t", required=true)
+  var targetIntervals: File = _
+
+  @Input(doc="bootstrap number", shortName="b", required=false)
+  var bootstrap: Int = 1
+
+  @Input(doc="downsampling step", shortName="ds", required=true)
+  var downsamplingStep: Double = _
+
+  @Input(doc="downsampling floor", shortName="df", required=false)
+  var downsamplingFloor: Double = 0.0
+
+  @Input(doc="downsampling ceiling", shortName="dc", required=false)
+  var downsamplingCeiling: Double = 1.0
+
+  @Input(doc="Reference fasta file", shortName="R", required=false)
+  var reference: File = new File("/seq/references/Homo_sapiens_assembly19/v1/Homo_sapiens_assembly19.fasta")
+
+  @Input(doc="dbSNP file", shortName="D", required=false)
+  var dbSNP: File = new File("/humgen/gsa-hpprojects/GATK/data/Comparisons/Validated/dbSNP/dbsnp_132_b37.leftAligned.vcf")
+
+  @Input(doc="project name", shortName="p", required=false)
+  var base: String = "prj"
+
+  def countLines(file: File):Int = {
+    var count: Int = 0
+    for (l <- fromFile(file).getLines) {
+      count = count + 1
+    }
+    return count
+  }
+
+  val queueLogDir: String = ".qlog/"
+  val outFile: String = "cov.out"
+
+  def script = {
+    val nIntervals = math.min(200, countLines(targetIntervals))
+
+    var f: Double = downsamplingCeiling
+    var i: Int = 1
+    while (f>=downsamplingFloor) {
+      var b: Int = bootstrap
+      while(b > 0) {
+        val file = swapExt(outFile, ".out", ".F" + i + "." + b + ".out")
+        add(cov(f, file))
+        b = b - 1
+      }
+      val snp_out = new File(base + ".F" + i + ".raw.vcf")
+      val filter_out = new File(base + ".F" + i + ".filtered.vcf")
+      val eval_out = new File(base + ".F" + i + ".eval")
+
+      add( snps(f, snp_out, nIntervals),
+           filter(snp_out, filter_out),
+           eval(filter_out, eval_out))
+
+      f = f - downsamplingStep
+      i = i + 1
+    }
+  }
+
+  trait CommandLineGATKArgs extends CommandLineGATK {
+    this.intervals :+= targetIntervals
+    this.jarFile = GATKjar
+    this.reference_sequence = reference
+    this.memoryLimit = 4
+  }
+
+  case class cov (fraction: Double, outFile: File) extends Percent20xCoverage with CommandLineGATKArgs {
+    this.input_file :+= input
+    this.out = outFile
+    this.ndrs = true
+    this.downsample_to_fraction = fraction
+    this.jobName = queueLogDir + outFile + ".cov"
+  }
+
+  case class snps (fraction: Double, outFile: File, nIntervals: Int) extends UnifiedGenotyper with CommandLineGATKArgs {
+    this.memoryLimit = 6
+    this.downsample_to_coverage = 600
+    this.genotype_likelihoods_model = org.broadinstitute.sting.gatk.walkers.genotyper.GenotypeLikelihoodsCalculationModel.Model.SNP
+    this.input_file :+= input
+    this.rodBind :+= RodBind("dbsnp", "VCF", dbSNP)
+    this.downsample_to_fraction = fraction
+    this.scatterCount = nIntervals
+    this.out = outFile
+    this.analysisName = outFile + "_snps"
+    this.jobName = queueLogDir + outFile
+  }
+
+  case class filter (inFile: File, outFile: File) extends VariantFiltration with CommandLineGATKArgs {
+    this.filterName ++= List("SNPSBFilter","SNPQDFilter","SNPHRunFilter")
+    this.filterExpression ++= List("\"SB>=0.10\"","\"QD<5.0\"","\"HRun>=4\"")
+    this.clusterWindowSize = 10
+    this.clusterSize = 3
+    this.variantVCF = inFile
+    this.out = outFile
+    this.analysisName = outFile + "_filter"
+    this.jobName = queueLogDir + outFile
+  }
+
+  case class eval (inFile: File, outFile: File) extends VariantEval with CommandLineGATKArgs {
+    this.noST = true
+    this.noEV = true
+    this.evalModule ++= List("TiTvVariantEvaluator", "CountVariants", "ValidationReport")
+    this.stratificationModule ++= List("EvalRod", "CompRod", "Novelty")
+    this.rodBind :+= RodBind("dbsnp", "VCF", dbSNP)
+    this.rodBind :+= RodBind("eval", "VCF", inFile)
+    this.out = outFile
+    this.analysisName = outFile + "_VariantEval"
+    this.jobName = queueLogDir + outFile
+  }
+}