Added utility shortcut method: getOriginalQualsInCycleOrder(read)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@3851 348d0f76-0448-11de-a6fe-93d51630548a
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@ -350,6 +350,20 @@ public class AlignmentUtils {
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return Utils.reverse(read.getBaseQualities());
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return Utils.reverse(read.getBaseQualities());
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}
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}
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/** Returns the array of original base qualitites (before recalibration) in the order the bases were read on the machine (i.e. always starting from
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* cycle 1). In other words, if the read is unmapped or aligned in the forward direction, the read's own base
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* qualities are returned as stored in the SAM record; if the read is aligned in the reverse direction, the array
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* of read's base qualitites is inverted (in this case new array is allocated and returned). If no original base qualities
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* are available this method will throw a runtime exception.
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* @param read
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* @return
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*/
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public static byte [] getOriginalQualsInCycleOrder(SAMRecord read) {
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if ( isReadUnmapped(read) || ! read.getReadNegativeStrandFlag() ) return read.getOriginalBaseQualities();
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return Utils.reverse(read.getOriginalBaseQualities());
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}
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/** Takes the alignment of the read sequence <code>readSeq</code> to the reference sequence <code>refSeq</code>
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/** Takes the alignment of the read sequence <code>readSeq</code> to the reference sequence <code>refSeq</code>
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* starting at 0-based position <code>refIndex</code> on the <code>refSeq</code> and specified by its <code>cigar</code>.
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* starting at 0-based position <code>refIndex</code> on the <code>refSeq</code> and specified by its <code>cigar</code>.
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* The last argument <code>readIndex</code> specifies 0-based position on the read where the alignment described by the
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* The last argument <code>readIndex</code> specifies 0-based position on the read where the alignment described by the
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