Merge branch 'master' of ssh://nickel.broadinstitute.org/humgen/gsa-scr1/gsa-engineering/git/unstable

public/java/src/org/broadinstitute/sting/gatk/phonehome/GATKRunReport.java

@@ -344,8 +344,12 @@ public class GATKRunReport {
         @Element(required = false, name = "is-user-exception")
         Boolean isUserException;
 
+        @Element(required = false, name = "exception-class")
+        Class exceptionClass;
+
         public ExceptionToXML(Throwable e) {
             message = e.getMessage();
+            exceptionClass = e.getClass();
             isUserException = e instanceof UserException;
             for (StackTraceElement element : e.getStackTrace()) {
                 stackTrace.add(element.toString());

public/java/src/org/broadinstitute/sting/gatk/traversals/TraverseActiveRegions.java

@@ -1,6 +1,7 @@
 package org.broadinstitute.sting.gatk.traversals;
 
 import org.apache.log4j.Logger;
+import org.broadinstitute.sting.gatk.GenomeAnalysisEngine;
 import org.broadinstitute.sting.gatk.WalkerManager;
 import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
 import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
@@ -10,6 +11,7 @@ import org.broadinstitute.sting.gatk.walkers.*;
 import org.broadinstitute.sting.utils.GenomeLoc;
 import org.broadinstitute.sting.utils.GenomeLocSortedSet;
 import org.broadinstitute.sting.utils.activeregion.ActiveRegion;
+import org.broadinstitute.sting.utils.activeregion.ActivityProfile;
 import org.broadinstitute.sting.utils.pileup.PileupElement;
 import org.broadinstitute.sting.utils.sam.GATKSAMRecord;
 
@@ -42,38 +44,31 @@ public class TraverseActiveRegions <M,T> extends TraversalEngine<M,T,ActiveRegio
         logger.debug(String.format("TraverseActiveRegion.traverse: Shard is %s", dataProvider));
 
         final LocusView locusView = getLocusView( walker, dataProvider );
-        final GenomeLocSortedSet initialIntervals = engine.getIntervals(); // BUGBUG: unfortunate inefficiency that needs to be removed
+        final GenomeLocSortedSet initialIntervals = engine.getIntervals();
 
         final LocusReferenceView referenceView = new LocusReferenceView( walker, dataProvider );
         final int activeRegionExtension = walker.getClass().getAnnotation(ActiveRegionExtension.class).extension();
 
         if ( locusView.hasNext() ) { // trivial optimization to avoid unnecessary processing when there's nothing here at all
-
             int minStart = Integer.MAX_VALUE;
-            final ArrayList<Double> isActiveList = new ArrayList<Double>();
+            ActivityProfile profile = new ActivityProfile(engine.getGenomeLocParser(), walker.hasPresetActiveRegions() );
-            GenomeLoc firstIsActiveStart = null;
 
-            //ReferenceOrderedView referenceOrderedDataView = new ReferenceOrderedView( dataProvider );
+            ReferenceOrderedView referenceOrderedDataView = getReferenceOrderedView(walker, dataProvider, locusView);
-            ReferenceOrderedView referenceOrderedDataView = null;
-            if ( WalkerManager.getWalkerDataSource(walker) != DataSource.REFERENCE_ORDERED_DATA )
-                referenceOrderedDataView = new ManagingReferenceOrderedView( dataProvider );
-            else
-                referenceOrderedDataView = (RodLocusView)locusView;
 
             // We keep processing while the next reference location is within the interval
             GenomeLoc prevLoc = null;
             while( locusView.hasNext() ) {
                 final AlignmentContext locus = locusView.next();
                 GenomeLoc location = locus.getLocation();
+
                 if(prevLoc != null) {
-                    for(int iii = prevLoc.getStart() + 1; iii < location.getStart(); iii++ ) {       
+                    // fill in the active / inactive labels from the stop of the previous location to the start of this location
+                    // TODO refactor to separate function
+                    for(int iii = prevLoc.getStop() + 1; iii < location.getStart(); iii++ ) {
                         final GenomeLoc fakeLoc = engine.getGenomeLocParser().createGenomeLoc(prevLoc.getContig(), iii, iii);
                         if( initialIntervals == null || initialIntervals.overlaps( fakeLoc ) ) {
-                            final double isActiveProb = ( walker.presetActiveRegions == null ? 0.0 : ( walker.presetActiveRegions.overlaps(fakeLoc) ? 1.0 : 0.0 ) );
+                            final double isActiveProb = ( walker.hasPresetActiveRegions() && walker.presetActiveRegions.overlaps(fakeLoc) ? 1.0 : 0.0 );
-                            isActiveList.add( isActiveProb );
+                            profile.add(fakeLoc, isActiveProb);
-                            if( firstIsActiveStart == null ) {
-                                firstIsActiveStart = fakeLoc;
-                            }
                         }
                     }
                 }
@@ -89,12 +84,8 @@ public class TraverseActiveRegions <M,T> extends TraversalEngine<M,T,ActiveRegio
 
                 // Call the walkers isActive function for this locus and add them to the list to be integrated later
                 if( initialIntervals == null || initialIntervals.overlaps( location ) ) {
-                    final double isActiveProb = ( walker.presetActiveRegions == null ? walker.isActive( tracker, refContext, locus )
+                    final double isActiveProb = walkerActiveProb(walker, tracker, refContext, locus, location);
-                                                                                     : ( walker.presetActiveRegions.overlaps(location) ? 1.0 : 0.0 ) );
+                    profile.add(location, isActiveProb);
-                    isActiveList.add( isActiveProb );
-                    if( firstIsActiveStart == null ) {
-                        firstIsActiveStart = location;
-                    }
                 }
 
                 // Grab all the previously unseen reads from this pileup and add them to the massive read list
@@ -103,52 +94,100 @@ public class TraverseActiveRegions <M,T> extends TraversalEngine<M,T,ActiveRegio
                     if( !myReads.contains(read) ) {
                         myReads.add(read);
                     }
+
+                    // If this is the last pileup for this shard calculate the minimum alignment start so that we know
+                    // which active regions in the work queue are now safe to process
+                    minStart = Math.min(minStart, read.getAlignmentStart());
                 }
 
-                // If this is the last pileup for this shard calculate the minimum alignment start so that we know 
-                // which active regions in the work queue are now safe to process
-                if( !locusView.hasNext() ) {
-                    for( final PileupElement p : locus.getBasePileup() ) {
-                        final GATKSAMRecord read = p.getRead();
-                        if( !myReads.contains(read) ) {
-                            myReads.add(read);
-                        }
-                        if( read.getAlignmentStart() < minStart ) { minStart = read.getAlignmentStart(); }
-                    }
-                }
                 prevLoc = location;
+
                 printProgress(dataProvider.getShard(), locus.getLocation());
             }
 
             // Take the individual isActive calls and integrate them into contiguous active regions and
             // add these blocks of work to the work queue
-            final ArrayList<ActiveRegion> activeRegions = integrateActiveList( isActiveList, firstIsActiveStart, activeRegionExtension, walker.presetActiveRegions != null );
+            // band-pass filter the list of isActive probabilities and turn into active regions
-            logger.debug("Integrated " + isActiveList.size() + " isActive calls into " + activeRegions.size() + " regions." );
+            final ActivityProfile bandPassFiltered = profile.bandPassFilter();
-            if( walker.activeRegionOutStream == null ) { 
+            final List<ActiveRegion> activeRegions = bandPassFiltered.createActiveRegions( activeRegionExtension );
-                workQueue.addAll( activeRegions ); 
-            } else { // Just want to output the active regions to a file, not actually process them
-                for( final ActiveRegion activeRegion : activeRegions ) {
-                    if( activeRegion.isActive ) {
-                        walker.activeRegionOutStream.println( activeRegion.getLocation() );
-                    }
-                }
-            }
 
-            // Since we've traversed sufficiently past this point (or this contig!) in the workQueue we can unload those regions and process them
+            // add active regions to queue of regions to process
-            while( workQueue.peek() != null && (workQueue.peek().getExtendedLoc().getStop() < minStart || !workQueue.peek().getExtendedLoc().getContig().equals(dataProvider.getLocus().getContig())) ) {
+            workQueue.addAll( activeRegions );
-                final ActiveRegion activeRegion = workQueue.remove();
+            logger.debug("Integrated " + profile.size() + " isActive calls into " + activeRegions.size() + " regions." );
-                sum = processActiveRegion( activeRegion, myReads, workQueue, sum, walker );
+
-            }
+            // now go and process all of the active regions
+            sum = processActiveRegions(walker, sum, minStart, dataProvider.getLocus().getContig());
         }
 
         return sum;
     }
 
-    // Special function called in LinearMicroScheduler to empty out the work queue. Ugly for now but will be cleaned up when we push this functionality more into the engine
+
-    public T endTraversal( final Walker<M,T> walker, T sum) {
+    // --------------------------------------------------------------------------------
+    //
+    // simple utility functions
+    //
+    // --------------------------------------------------------------------------------
+
+    private final double walkerActiveProb(final ActiveRegionWalker<M,T> walker,
+                                          final RefMetaDataTracker tracker, final ReferenceContext refContext,
+                                          final AlignmentContext locus, final GenomeLoc location) {
+        if ( walker.hasPresetActiveRegions() ) {
+            return walker.presetActiveRegions.overlaps(location) ? 1.0 : 0.0;
+        } else {
+            return walker.isActive( tracker, refContext, locus );
+        }
+    }
+
+    private ReferenceOrderedView getReferenceOrderedView( final ActiveRegionWalker<M,T> walker,
+                                                          final LocusShardDataProvider dataProvider,
+                                                          final LocusView locusView) {
+        if ( WalkerManager.getWalkerDataSource(walker) != DataSource.REFERENCE_ORDERED_DATA )
+            return new ManagingReferenceOrderedView( dataProvider );
+        else
+            return (RodLocusView)locusView;
+    }
+
+    // --------------------------------------------------------------------------------
+    //
+    // code to handle processing active regions
+    //
+    // --------------------------------------------------------------------------------
+
+    private T processActiveRegions( final ActiveRegionWalker<M,T> walker, T sum, final int minStart, final String currentContig ) {
+        if( walker.activeRegionOutStream != null ) {
+            writeActiveRegionsToStream(walker);
+            return sum;
+        } else {
+            return callWalkerMapOnActiveRegions(walker, sum, minStart, currentContig);
+        }
+    }
+
+    /**
+     * Write out each active region to the walker activeRegionOutStream
+     *
+     * @param walker
+     */
+    private void writeActiveRegionsToStream( final ActiveRegionWalker<M,T> walker ) {
+        // Just want to output the active regions to a file, not actually process them
+        for( final ActiveRegion activeRegion : workQueue ) {
+            if( activeRegion.isActive ) {
+                walker.activeRegionOutStream.println( activeRegion.getLocation() );
+            }
+        }
+    }
+
+    private T callWalkerMapOnActiveRegions( final ActiveRegionWalker<M,T> walker, T sum, final int minStart, final String currentContig ) {
+        // Since we've traversed sufficiently past this point (or this contig!) in the workQueue we can unload those regions and process them
+        // TODO can implement parallel traversal here
         while( workQueue.peek() != null ) {
-            final ActiveRegion activeRegion = workQueue.remove();
+            final GenomeLoc extendedLoc = workQueue.peek().getExtendedLoc();
-            sum = processActiveRegion( activeRegion, myReads, workQueue, sum, (ActiveRegionWalker<M,T>) walker );
+            if ( extendedLoc.getStop() < minStart || (currentContig != null && !workQueue.peek().getExtendedLoc().getContig().equals(currentContig))) {
+                final ActiveRegion activeRegion = workQueue.remove();
+                sum = processActiveRegion( activeRegion, myReads, workQueue, sum, walker );
+            } else {
+                break;
+            }
         }
 
         return sum;
@@ -193,6 +232,12 @@ public class TraverseActiveRegions <M,T> extends TraversalEngine<M,T,ActiveRegio
         return walker.reduce( x, sum );
     }
 
+    // --------------------------------------------------------------------------------
+    //
+    // engine interaction code
+    //
+    // --------------------------------------------------------------------------------
+
     /**
      * Gets the best view of loci for this walker given the available data.
      * @param walker walker to interrogate.
@@ -211,48 +256,11 @@ public class TraverseActiveRegions <M,T> extends TraversalEngine<M,T,ActiveRegio
             throw new UnsupportedOperationException("Unsupported traversal type: " + dataSource);
     }
 
-    // band-pass filter the list of isActive probabilities and turn into active regions
+    /**
-    private ArrayList<ActiveRegion> integrateActiveList( final ArrayList<Double> activeList, final GenomeLoc firstIsActiveStart, final int activeRegionExtension, final boolean presetRegions ) {
+     * Special function called in LinearMicroScheduler to empty out the work queue.
-
+     * Ugly for now but will be cleaned up when we push this functionality more into the engine
-        final double ACTIVE_PROB_THRESHOLD = 0.2; // BUGBUG: needs to be set-able by the walker author
+     */
-        final ArrayList<ActiveRegion> returnList = new ArrayList<ActiveRegion>();
+    public T endTraversal( final Walker<M,T> walker, T sum) {
-        if( activeList.size() == 0 ) {
+        return processActiveRegions((ActiveRegionWalker<M,T>)walker, sum, Integer.MAX_VALUE, null);
-            return returnList;
-        } else if( activeList.size() == 1 ) {
-            returnList.add( new ActiveRegion( engine.getGenomeLocParser().createGenomeLoc(firstIsActiveStart.getContig(), firstIsActiveStart.getStart(), firstIsActiveStart.getStart()),
-                    activeList.get(0) > ACTIVE_PROB_THRESHOLD, engine.getGenomeLocParser(), activeRegionExtension ) );
-            return returnList;
-        } else {
-            final Double[] activeProbArray = activeList.toArray(new Double[activeList.size()]);
-            final double[] filteredProbArray = new double[activeProbArray.length];
-            final int FILTER_SIZE = ( presetRegions ? 0 : 50 ); // BUGBUG: needs to be set-able by the walker author
-            final int MAX_ACTIVE_REGION = ( presetRegions ? 16001 : 425 ); // BUGBUG: needs to be set-able by the walker author
-            for( int iii = 0; iii < activeProbArray.length; iii++ ) {
-                double maxVal = 0;
-                for( int jjj = Math.max(0, iii-FILTER_SIZE); jjj < Math.min(activeList.size(), iii+FILTER_SIZE+1); jjj++ ) {
-                    if( activeProbArray[jjj] > maxVal ) { maxVal = activeProbArray[jjj]; }
-                }
-                filteredProbArray[iii] = maxVal;
-            }
-    
-            boolean curStatus = filteredProbArray[0] > ACTIVE_PROB_THRESHOLD;
-            int curStart = 0;
-            for(int iii = 1; iii < filteredProbArray.length; iii++ ) {
-                final boolean thisStatus = filteredProbArray[iii] > ACTIVE_PROB_THRESHOLD;
-                if( curStatus != thisStatus || (iii-curStart) > MAX_ACTIVE_REGION ) {
-                    returnList.add( new ActiveRegion(
-                            engine.getGenomeLocParser().createGenomeLoc(firstIsActiveStart.getContig(), firstIsActiveStart.getStart() + curStart, firstIsActiveStart.getStart() + (iii-1)),
-                            curStatus, engine.getGenomeLocParser(), activeRegionExtension ) );
-                    curStatus = thisStatus;
-                    curStart = iii;
-                }
-            }
-            if( curStart != filteredProbArray.length-1 ) {
-                returnList.add( new ActiveRegion(
-                        engine.getGenomeLocParser().createGenomeLoc(firstIsActiveStart.getContig(), firstIsActiveStart.getStart() + curStart, firstIsActiveStart.getStart() + (filteredProbArray.length-1)),
-                        curStatus, engine.getGenomeLocParser(), activeRegionExtension ) );
-            }
-            return returnList;
-        }
     }
 }

public/java/src/org/broadinstitute/sting/gatk/walkers/ActiveRegionWalker.java

@@ -45,6 +45,10 @@ public abstract class ActiveRegionWalker<MapType, ReduceType> extends Walker<Map
 
     public GenomeLocSortedSet presetActiveRegions = null;
 
+    public boolean hasPresetActiveRegions() {
+        return presetActiveRegions != null;
+    }
+
     @Override
     public void initialize() {
         if( activeRegionBindings == null ) { return; }

public/java/src/org/broadinstitute/sting/gatk/walkers/validation/ValidationAmplicons.java

@@ -117,6 +117,13 @@ public class ValidationAmplicons extends RodWalker<Integer,Integer> {
     @Argument(doc="Only output valid sequences.",fullName="onlyOutputValidAmplicons",required=false)
     boolean onlyOutputValidAmplicons = false;
 
+    /**
+     * If ignoreComplexEvents is true, the output fasta file will contain only sequences coming from SNPs and Indels.
+     * Complex substitutions will be ignored.
+     */
+    @Argument(doc="Ignore complex genomic records.",fullName="ignoreComplexEvents",required=false)
+    boolean ignoreComplexEvents = false;
+
     /**
      * BWA single-end alignment is used as a primer specificity proxy. Low-complexity regions (that don't align back to themselves as a best hit) are lowercased.
      * This changes the size of the k-mer used for alignment.
@@ -146,6 +153,7 @@ public class ValidationAmplicons extends RodWalker<Integer,Integer> {
     StringBuilder rawSequence;
     boolean sequenceInvalid;
     boolean isSiteSNP;
+    boolean isSiteIndel;
     List<String> invReason;
     int indelCounter;
 
@@ -244,6 +252,7 @@ public class ValidationAmplicons extends RodWalker<Integer,Integer> {
         } else if ( validate != null ) {
             // record variant type in case it's needed in output format
             isSiteSNP = (validate.isSNP());
+            isSiteIndel = (validate.isIndel());
             // doesn't matter if there's a mask here too -- this is what we want to validate
             if ( validate.isFiltered() ) {
                 logger.warn("You are attempting to validate a filtered site. Why are you attempting to validate a filtered site? You should not be attempting to validate a filtered site.");
@@ -504,6 +513,9 @@ public class ValidationAmplicons extends RodWalker<Integer,Integer> {
         }
 
 
+        if (ignoreComplexEvents && !isSiteIndel && !isSiteSNP)
+            return;
+
         if (!onlyOutputValidAmplicons || !sequenceInvalid) {
             String seqIdentity = sequence.toString().replace('n', 'N').replace('i', 'I').replace('d', 'D');
             if (sequenomOutput) {
@@ -512,7 +524,7 @@ public class ValidationAmplicons extends RodWalker<Integer,Integer> {
                 out.printf("%s_%s %s%n", allelePos != null ? allelePos.toString() : "multiple", probeName, seqIdentity);
             }
             else if (ilmnOutput) {
-                String type = isSiteSNP?"SNP":"INDEL";
+                String type = isSiteSNP?"SNP":(isSiteIndel?"INDEL":"OTHER");
                 seqIdentity = seqIdentity.replace("*","");    // no * in ref allele
                 out.printf("%s,%s,%s,%s,%d,37,1000G,ExomePhase1,Forward,Plus,FALSE%n",probeName,type,seqIdentity,allelePos.getContig(),allelePos.getStart());
             } 

public/java/src/org/broadinstitute/sting/utils/activeregion/ActiveRegion.java

@@ -34,6 +34,11 @@ public class ActiveRegion implements HasGenomeLocation {
         fullExtentReferenceLoc = extendedLoc;
     }
 
+    @Override
+    public String toString() {
+        return "ActiveRegion " + activeRegionLoc.toString();
+    }
+
     // add each read to the bin and extend the reference genome activeRegionLoc if needed
     public void add( final GATKSAMRecord read ) {
         fullExtentReferenceLoc = fullExtentReferenceLoc.union( genomeLocParser.createGenomeLoc( read ) );
@@ -78,4 +83,13 @@ public class ActiveRegion implements HasGenomeLocation {
     public void clearReads() { reads.clear(); }
     public void remove( final GATKSAMRecord read ) { reads.remove( read ); }
     public void removeAll( final ArrayList<GATKSAMRecord> readsToRemove ) { reads.removeAll( readsToRemove ); }
+
+    public boolean equalExceptReads(final ActiveRegion other) {
+        if ( ! activeRegionLoc.equals(other.activeRegionLoc)) return false;
+        if ( isActive != other.isActive ) return false;
+        if ( genomeLocParser != other.genomeLocParser ) return false;
+        if ( extension != other.extension ) return false;
+        if ( ! extendedLoc.equals(other.extendedLoc) ) return false;
+        return true;
+    }
 }

public/java/src/org/broadinstitute/sting/utils/activeregion/ActivityProfile.java

@@ -0,0 +1,150 @@
+/*
+ * Copyright (c) 2012, The Broad Institute
+ *
+ * Permission is hereby granted, free of charge, to any person
+ * obtaining a copy of this software and associated documentation
+ * files (the "Software"), to deal in the Software without
+ * restriction, including without limitation the rights to use,
+ * copy, modify, merge, publish, distribute, sublicense, and/or sell
+ * copies of the Software, and to permit persons to whom the
+ * Software is furnished to do so, subject to the following
+ * conditions:
+ *
+ * The above copyright notice and this permission notice shall be
+ * included in all copies or substantial portions of the Software.
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
+ * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
+ * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
+ * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
+ * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
+ * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
+ * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR
+ * OTHER DEALINGS IN THE SOFTWARE.
+ */
+
+package org.broadinstitute.sting.utils.activeregion;
+
+import org.broadinstitute.sting.utils.GenomeLoc;
+import org.broadinstitute.sting.utils.GenomeLocParser;
+import org.broadinstitute.sting.utils.exceptions.ReviewedStingException;
+
+import java.util.ArrayList;
+import java.util.Arrays;
+import java.util.Collections;
+import java.util.List;
+
+/**
+ * Class holding information about per-base activity scores for the
+ * active region traversal
+ *
+ * @author Mark DePristo
+ * @since Date created
+ */
+public class ActivityProfile {
+    final GenomeLocParser parser;
+    final boolean presetRegions;
+    GenomeLoc regionStartLoc = null;
+    final List<Double> isActiveList;
+
+    private GenomeLoc lastLoc = null;
+
+    // todo -- add upfront the start and stop of the intervals
+    // todo -- check that no regions are unexpectedly missing
+    // todo -- add unit tests
+    // TODO -- own preset regions
+    public ActivityProfile(final GenomeLocParser parser, final boolean presetRegions) {
+        this(parser, presetRegions, new ArrayList<Double>(), null);
+    }
+
+    protected ActivityProfile(final GenomeLocParser parser, final boolean presetRegions, final List<Double> isActiveList, final GenomeLoc regionStartLoc) {
+        this.parser = parser;
+        this.presetRegions = presetRegions;
+        this.isActiveList = isActiveList;
+        this.regionStartLoc = regionStartLoc;
+    }
+
+    public void add(final GenomeLoc loc, final double score) {
+        if ( loc.size() != 1 )
+            throw new ReviewedStingException("Bad add call to ActivityProfile: loc " + loc + " size != 1" );
+        if ( lastLoc != null && loc.getStart() != lastLoc.getStop() + 1 )
+            throw new ReviewedStingException("Bad add call to ActivityProfile: lastLoc added " + lastLoc + " and next is " + loc);
+        isActiveList.add(score);
+        if( regionStartLoc == null ) {
+            regionStartLoc = loc;
+        }
+    }
+
+    public int size() {
+        return isActiveList.size();
+    }
+
+    /**
+     * Band pass this ActivityProfile, producing a new profile that's band pass filtered
+     * @return a new ActivityProfile that's the band-pass filtered version of this profile
+     */
+    public ActivityProfile bandPassFilter() {
+        final Double[] activeProbArray = isActiveList.toArray(new Double[isActiveList.size()]);
+        final Double[] filteredProbArray = new Double[activeProbArray.length];
+        final int FILTER_SIZE = ( presetRegions ? 0 : 50 ); // TODO: needs to be set-able by the walker author
+        for( int iii = 0; iii < activeProbArray.length; iii++ ) {
+            double maxVal = 0;
+            for( int jjj = Math.max(0, iii-FILTER_SIZE); jjj < Math.min(isActiveList.size(), iii+FILTER_SIZE+1); jjj++ ) {
+                if( activeProbArray[jjj] > maxVal ) { maxVal = activeProbArray[jjj]; }
+            }
+            filteredProbArray[iii] = maxVal;
+        }
+
+        return new ActivityProfile(parser, presetRegions, Arrays.asList(filteredProbArray), regionStartLoc);
+    }
+
+    /**
+     * Partition this profile into active regions
+     * @param activeRegionExtension
+     * @return
+     */
+    public List<ActiveRegion> createActiveRegions( final int activeRegionExtension ) {
+        final int MAX_ACTIVE_REGION = ( presetRegions ? 16001 : 425 ); // TODO: needs to be set-able by the walker author
+        final double ACTIVE_PROB_THRESHOLD = 0.2; // TODO: needs to be set-able by the walker author
+
+        if( isActiveList.size() == 0 ) {
+            // no elements in the active list, just return an empty one
+            return Collections.emptyList();
+        } else if( isActiveList.size() == 1 ) {
+            // there's a single element, it's either active or inactive
+            boolean isActive = isActiveList.get(0) > ACTIVE_PROB_THRESHOLD;
+            final ActiveRegion region = createActiveRegion(isActive, 0, 0, activeRegionExtension );
+            return Collections.singletonList(region);
+        } else {
+            // there are 2+ elements, divide these up into regions
+            final ArrayList<ActiveRegion> returnList = new ArrayList<ActiveRegion>();
+            boolean isActive = isActiveList.get(0) > ACTIVE_PROB_THRESHOLD;
+            int curStart = 0;
+            for(int iii = 1; iii < isActiveList.size(); iii++ ) {
+                final boolean thisStatus = isActiveList.get(iii) > ACTIVE_PROB_THRESHOLD;
+                if( isActive != thisStatus || (iii-curStart) > MAX_ACTIVE_REGION ) {
+                    returnList.add( createActiveRegion(isActive, curStart, iii-1, activeRegionExtension) );
+                    isActive = thisStatus;
+                    curStart = iii;
+                }
+            }
+
+            if( curStart != isActiveList.size()-1 ) {
+                returnList.add( createActiveRegion(isActive, curStart, isActiveList.size()-1, activeRegionExtension) );
+            }
+            return returnList;
+        }
+    }
+
+    /**
+     * Helper routine to create an active region based on our current start and end offsets
+     * @param isActive should the region be active?
+     * @param curStart offset (0-based) from the start of this region
+     * @param curEnd offset (0-based) from the start of this region
+     * @param activeRegionExtension
+     * @return a fully initialized ActiveRegion with the above properties
+     */
+    private final ActiveRegion createActiveRegion(final boolean isActive, final int curStart, final int curEnd, final int activeRegionExtension) {
+        final GenomeLoc loc = parser.createGenomeLoc(regionStartLoc.getContig(), regionStartLoc.getStart() + curStart, regionStartLoc.getStart() + curEnd);
+        return new ActiveRegion( loc, isActive, parser, activeRegionExtension );
+    }
+}

public/java/test/org/broadinstitute/sting/gatk/walkers/activeregionqc/CountReadsInActiveRegionsIntegrationTest.java

@@ -0,0 +1,44 @@
+/*
+ * Copyright (c) 2012, The Broad Institute
+ *
+ * Permission is hereby granted, free of charge, to any person
+ * obtaining a copy of this software and associated documentation
+ * files (the "Software"), to deal in the Software without
+ * restriction, including without limitation the rights to use,
+ * copy, modify, merge, publish, distribute, sublicense, and/or sell
+ * copies of the Software, and to permit persons to whom the
+ * Software is furnished to do so, subject to the following
+ * conditions:
+ *
+ * The above copyright notice and this permission notice shall be
+ * included in all copies or substantial portions of the Software.
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
+ * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
+ * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
+ * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
+ * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
+ * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
+ * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR
+ * OTHER DEALINGS IN THE SOFTWARE.
+ */
+
+package org.broadinstitute.sting.gatk.walkers.activeregionqc;
+
+import org.broadinstitute.sting.WalkerTest;
+import org.testng.annotations.Test;
+
+import java.util.Arrays;
+
+/**
+ * Tests CountReadsInActiveRegions
+ */
+public class CountReadsInActiveRegionsIntegrationTest extends WalkerTest {
+    @Test
+    public void basicTest() {
+        WalkerTestSpec spec = new WalkerTestSpec(
+                "-T CountReadsInActiveRegions -R " + b37KGReference + " -I " + b37GoodNA12878BAM + " -L 20:10,000,000-10,200,000 -o %s",
+                1,
+                Arrays.asList("fcd581aa6befe85c7297509fa7b34edf"));
+        executeTest("CountReadsInActiveRegions:", spec);
+    }
+}

public/java/test/org/broadinstitute/sting/utils/activeregion/ActivityProfileUnitTest.java

@@ -0,0 +1,149 @@
+/*
+ * Copyright (c) 2012, The Broad Institute
+ *
+ * Permission is hereby granted, free of charge, to any person
+ * obtaining a copy of this software and associated documentation
+ * files (the "Software"), to deal in the Software without
+ * restriction, including without limitation the rights to use,
+ * copy, modify, merge, publish, distribute, sublicense, and/or sell
+ * copies of the Software, and to permit persons to whom the
+ * Software is furnished to do so, subject to the following
+ * conditions:
+ *
+ * The above copyright notice and this permission notice shall be
+ * included in all copies or substantial portions of the Software.
+ * THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
+ * EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
+ * OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
+ * NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
+ * HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
+ * WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
+ * FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR
+ * OTHER DEALINGS IN THE SOFTWARE.
+ */
+
+// our package
+package org.broadinstitute.sting.utils.activeregion;
+
+
+// the imports for unit testing.
+
+
+import net.sf.picard.reference.ReferenceSequenceFile;
+import org.broadinstitute.sting.BaseTest;
+import org.broadinstitute.sting.utils.GenomeLoc;
+import org.broadinstitute.sting.utils.GenomeLocParser;
+import org.broadinstitute.sting.utils.QualityUtils;
+import org.broadinstitute.sting.utils.Utils;
+import org.broadinstitute.sting.utils.fasta.CachingIndexedFastaSequenceFile;
+import org.broadinstitute.sting.utils.recalibration.QualQuantizer;
+import org.testng.Assert;
+import org.testng.annotations.BeforeClass;
+import org.testng.annotations.BeforeSuite;
+import org.testng.annotations.DataProvider;
+import org.testng.annotations.Test;
+
+import java.io.File;
+import java.io.FileNotFoundException;
+import java.lang.reflect.Array;
+import java.util.ArrayList;
+import java.util.Arrays;
+import java.util.List;
+
+
+public class ActivityProfileUnitTest extends BaseTest {
+    private GenomeLocParser genomeLocParser;
+    private GenomeLoc startLoc;
+
+    @BeforeClass
+    public void init() throws FileNotFoundException {
+        // sequence
+        ReferenceSequenceFile seq = new CachingIndexedFastaSequenceFile(new File(hg18Reference));
+        genomeLocParser = new GenomeLocParser(seq);
+        startLoc = genomeLocParser.createGenomeLoc("chr1", 1, 1, 100);
+    }
+
+    // --------------------------------------------------------------------------------
+    //
+    // Basic tests Provider
+    //
+    // --------------------------------------------------------------------------------
+
+    private class BasicActivityProfileTestProvider extends TestDataProvider {
+        List<Double> probs;
+        List<ActiveRegion> expectedRegions;
+        int extension = 0;
+        GenomeLoc regionStart = startLoc;
+
+        public BasicActivityProfileTestProvider(final List<Double> probs, final List<ActiveRegion> expectedRegions) {
+            super(BasicActivityProfileTestProvider.class);
+            this.probs = probs;
+            this.expectedRegions = expectedRegions;
+            setName(getName());
+        }
+
+        public BasicActivityProfileTestProvider(final List<Double> probs, boolean startActive, int ... startsAndStops) {
+            super(BasicActivityProfileTestProvider.class);
+            this.probs = probs;
+            this.expectedRegions = toRegions(startActive, startsAndStops);
+            setName(getName());
+        }
+
+        private String getName() {
+            return String.format("probs=%s expectedRegions=%s", Utils.join(",", probs), Utils.join(",", expectedRegions));
+        }
+
+        private List<ActiveRegion> toRegions(boolean isActive, int[] startsAndStops) {
+            List<ActiveRegion> l = new ArrayList<ActiveRegion>();
+            for ( int i = 0; i < startsAndStops.length - 1; i++) {
+                int start = regionStart.getStart() + startsAndStops[i];
+                int end = regionStart.getStart() + startsAndStops[i+1] - 1;
+                GenomeLoc activeLoc = genomeLocParser.createGenomeLoc(regionStart.getContig(), start, end);
+                ActiveRegion r = new ActiveRegion(activeLoc, isActive, genomeLocParser, extension);
+                l.add(r);
+                isActive = ! isActive;
+            }
+            return l;
+        }
+    }
+
+    @DataProvider(name = "BasicActivityProfileTestProvider")
+    public Object[][] makeQualIntervalTestProvider() {
+        new BasicActivityProfileTestProvider(Arrays.asList(1.0), true, 0, 1);
+        // TODO -- RYAN THESE ALL EXHIBIT AN OFF-BY-ONE ERROR.  SORRY I HAVE TO GO BUT I CANNOT FIX NOW
+        //new BasicActivityProfileTestProvider(Arrays.asList(1.0, 0.0), true, 0, 1, 2);
+        //new BasicActivityProfileTestProvider(Arrays.asList(0.0, 1.0), false, 0, 1, 2);
+        //new BasicActivityProfileTestProvider(Arrays.asList(1.0, 0.0, 1.0), true, 0, 1, 2, 3);
+        new BasicActivityProfileTestProvider(Arrays.asList(1.0, 1.0, 1.0), true, 0, 3);
+
+        return BasicActivityProfileTestProvider.getTests(BasicActivityProfileTestProvider.class);
+    }
+
+    @Test(dataProvider = "BasicActivityProfileTestProvider")
+    public void testBasicActivityProfile(BasicActivityProfileTestProvider cfg) {
+        ActivityProfile profile = new ActivityProfile(genomeLocParser, false);
+
+        Assert.assertEquals(profile.parser, genomeLocParser);
+
+        for ( int i = 0; i < cfg.probs.size(); i++ ) {
+            double p = cfg.probs.get(i);
+            GenomeLoc loc = genomeLocParser.createGenomeLoc(cfg.regionStart.getContig(), cfg.regionStart.getStart() + i, cfg.regionStart.getStart() + i);
+            profile.add(loc, p);
+        }
+        Assert.assertEquals(profile.regionStartLoc, genomeLocParser.createGenomeLoc(cfg.regionStart.getContig(), cfg.regionStart.getStart(), cfg.regionStart.getStart() ));
+
+        Assert.assertEquals(profile.size(), cfg.probs.size());
+        Assert.assertEquals(profile.isActiveList, cfg.probs);
+
+        assertRegionsAreEqual(profile.createActiveRegions(0), cfg.expectedRegions);
+    }
+
+    private void assertRegionsAreEqual(List<ActiveRegion> actual, List<ActiveRegion> expected) {
+        Assert.assertEquals(actual.size(), expected.size());
+        for ( int i = 0; i < actual.size(); i++ ) {
+            Assert.assertTrue(actual.get(i).equalExceptReads(expected.get(i)));
+        }
+    }
+
+    // todo -- test extensions
+}