New:

Minor changes to CoverageAndPowerWalker bootstrapping (faster selection of indeces).

Entirely new Aritifical Pool Walker (ArtificialPoolWalkerMk2), will likely replace ArtificialPoolWalker on the next commit. Adapted the method of sampling, and added a helper context class: ArtificialPoolContext which carries much of the burden of calculation and data handling for the walker. The walker itself maps and reduces ArtificialPoolContexts.

Cheers!






git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@1461 348d0f76-0448-11de-a6fe-93d51630548a

java/src/org/broadinstitute/sting/playground/gatk/walkers/poolseq/AnalyzePowerWalker.java

@@ -29,6 +29,8 @@ public class AnalyzePowerWalker extends CoverageAndPowerWalker{
         String pathToSyzygyFile = null;
     @Argument(fullName = "ColumnOffset", shortName = "co", doc = "Offset of column containing the power in the pf", required = true)
         int colOffset = 0;
+    @Argument(fullName = "linesToClear", shortName="clr", doc = "Clear so many lines from the read file before starting (default - just the header line)", required = false)
+        int clrLines = 1;
 
     BufferedReader syzyFileReader;
     final String pfFileDelimiter = " ";
@@ -40,7 +42,9 @@ public class AnalyzePowerWalker extends CoverageAndPowerWalker{
         super.initialize();
         try {
             syzyFileReader = new BufferedReader(new FileReader(pathToSyzygyFile));
+            for(int clear = 0; clear < clrLines; clear++) {
                 syzyFileReader.readLine();
+            }
         } catch (FileNotFoundException e) {
             String newErrMsg = "Syzygy input file " + pathToSyzygyFile + " could be incorrect. File not found.";
             throw new StingException(newErrMsg,e);
@@ -69,8 +73,8 @@ public class AnalyzePowerWalker extends CoverageAndPowerWalker{
             if(!syzyFileIsReady) {
                 throw new StingException("Input file reader was not ready before an attempt to read from it.");
             } else if(!outOfLinesInSyzyFile) {
-                double syzyPow = getSyzyPowFromFile();
+                Pair<Double,String> syzyPow = getSyzyPowFromFile();
-                out.printf("%s: %d %d %f %f%n", context.getLocation(), context.getReads().size(),powpair.second,powpair.first,syzyPow);
+                out.printf("%s: %d %d %f %f (%s)%n", context.getLocation(), context.getReads().size(),powpair.second,powpair.first,syzyPow.first,syzyPow.second);
             } else {
                 out.printf("%s: %d %d %f%n", context.getLocation(), context.getReads().size(),powpair.second,powpair.first);
             }
@@ -79,7 +83,7 @@ public class AnalyzePowerWalker extends CoverageAndPowerWalker{
         return context.getReads().size();
     }
 
-    public double getSyzyPowFromFile() {
+    public Pair<Double,String> getSyzyPowFromFile() {
         String thisLine = null;
         try {
             thisLine = syzyFileReader.readLine();
@@ -87,15 +91,17 @@ public class AnalyzePowerWalker extends CoverageAndPowerWalker{
             String newErrMsg = "Ran out of lines in the syzyfile; further output of Syzygy power will be suppressed.";
             outOfLinesInSyzyFile=true;
             logger.warn(newErrMsg + " " + e.toString());
-            return -1.1;
+            return new Pair(-1.1, "Printing Stops Here");
         }
 
+        String chromPos = null;
         StringTokenizer lineTokenizer = new StringTokenizer(thisLine, pfFileDelimiter);
         try {
-            for(int j = 0; j < colOffset; j++) {
+            chromPos = lineTokenizer.nextToken();
+            for(int j = 1; j < colOffset; j++) {
                 lineTokenizer.nextToken();
             }
-            return (Double.valueOf(lineTokenizer.nextToken())/100.0);
+            return new Pair((Double.valueOf(lineTokenizer.nextToken())/100.0),chromPos);
         } catch (NoSuchElementException e) {
             String errMsg = "The given column offset for the pool, " + colOffset + " exceeded the number of entries in the file " + pathToSyzygyFile;
             throw new StingException(errMsg);

java/src/org/broadinstitute/sting/playground/gatk/walkers/poolseq/ArtificalPoolWalkerMk2.java

@@ -0,0 +1,146 @@
+package org.broadinstitute.sting.playground.gatk.walkers.poolseq;
+
+import org.broadinstitute.sting.gatk.walkers.LocusWalker;
+import org.broadinstitute.sting.gatk.walkers.genotyper.SingleSampleGenotyper;
+import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
+import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
+import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
+import net.sf.samtools.SAMFileWriter;
+import net.sf.samtools.SAMRecord;
+import org.broadinstitute.sting.playground.utils.ArtificialPoolContext;
+import org.broadinstitute.sting.utils.cmdLine.Argument;
+import org.broadinstitute.sting.utils.StingException;
+
+import java.io.FileOutputStream;
+import java.io.PrintWriter;
+import java.io.FileNotFoundException;
+import java.io.IOException;
+import java.util.Set;
+import java.util.List;
+import java.util.ListIterator;
+import java.util.LinkedList;
+
+/**
+ * Created by IntelliJ IDEA.
+ * User: chartl
+ * Date: Aug 26, 2009
+ * Time: 11:28:26 AM
+ * To change this template use File | Settings | File Templates.
+ */
+public class ArtificalPoolWalkerMk2 extends LocusWalker<ArtificialPoolContext, ArtificialPoolContext> {
+
+    @Argument(fullName = "AuxOutputFile", shortName = "af", doc = "Auxiliary file for genotyp & coverage output", required = true)
+        String auxFilePath = null;
+    @Argument(fullName = "OutputBamFile", shortName = "of", doc = "Output to this file rather than standard output", required = false)
+        SAMFileWriter outputBamFile = null;
+
+    public void initialize() {
+    }
+
+    public ArtificialPoolContext reduceInit() { // try to initialize the file writer
+        ArtificialPoolContext apContext = new ArtificialPoolContext();
+        apContext.setSingleSampleGenotyper(new SingleSampleGenotyper());
+        apContext.setReadGroupSets(getToolkit().getMergedReadGroupsByReaders());
+        apContext.setAuxWriter(initializeAuxFileWriter(apContext.getTotalNumberOfPeople()));
+        apContext.setSAMFileWriter(outputBamFile);
+        apContext.initializeSSG();
+
+        return apContext;
+    }
+
+    public ArtificialPoolContext map(RefMetaDataTracker tracker, ReferenceContext ref, AlignmentContext context) {
+        return new ArtificialPoolContext(tracker,ref,context);
+    }
+
+    public ArtificialPoolContext reduce(ArtificialPoolContext mapCon, ArtificialPoolContext redCon){
+        ArtificialPoolContext updatedContext = ArtificialPoolContext.mapReduceMerge(mapCon,redCon);
+        List<SAMRecord>[] newReads = updatedContext.splitReadsByGroup(updatedContext.getNewReads());
+        long[] newCvg = updateRunningCoverage(updatedContext.getRunningCoverage(), getCoverageByGroup(newReads));
+        updatedContext.setRunningCoverage(newCvg);
+        List<SAMRecord>[] sampledReads = ArtificialPoolContext.sampleReads(newReads,runningCoverageToDouble(newCvg));
+        printToFiles(sampledReads,updatedContext);
+        return updatedContext;
+    }
+
+    // Helper methods follow
+
+    private PrintWriter initializeAuxFileWriter(int nFiles) {
+        PrintWriter auxFileWriter;
+        try {
+            auxFileWriter = new PrintWriter(new FileOutputStream(auxFilePath));
+            auxFileWriter.println(createAuxFileHeader(nFiles));
+        } catch(FileNotFoundException e) {
+            String errmsg = "The filepath you entered "+auxFilePath+" could not be opened. Please double-check that the input is correct.";
+            throw new StingException(errmsg, e);
+        } catch(IOException e) {
+            String errmsg = "The file you entered "+auxFilePath+" could not be written to. Please check your permissions to write to this file.";
+            throw new StingException(errmsg,e);
+        }
+
+        return auxFileWriter;
+    }
+
+    private String createAuxFileHeader(int nFiles) {
+        String sp = "      ";
+        String st1 = "Chrom:Pos" + sp;
+        String st2 = "";
+        for(int j = 0; j < nFiles; j++) {
+            st2 = st2 + "Pers " + j + " Gen" + sp; // short for "genotype of person j at this location"
+            st2 = st2 + "Pers " + j + " Conf" + sp; // short for "confidence in genotype call of ..."
+            st2 = st2 + "Pers " + j + " NewCvg" + sp; // short for "coverage of person j at this location"
+        }
+        String st3 = "TotalCvg";
+
+        return st1+st2+st3;
+    }
+
+    private int[] getCoverageByGroup(List<SAMRecord>[] readsByGroup) {
+        int[] coverage = new int[readsByGroup.length];
+        for(int iterator = 0; iterator < readsByGroup.length; iterator ++) {
+            coverage[iterator] = readsByGroup[iterator].size();
+        }
+
+        return coverage;
+    }
+
+    private long[] updateRunningCoverage(long[] cvgUpToNow, int[] newCvgByGroup) {
+        long[] newCvg = new long[cvgUpToNow.length];
+        for(int iter = 0; iter < cvgUpToNow.length; iter++) {
+            newCvg[iter] = cvgUpToNow[iter] + newCvgByGroup[iter];
+        }
+
+        return newCvg;
+    }
+
+    private double[] runningCoverageToDouble(long[] cvg) {
+        double[] avgProp = new double[cvg.length];
+        long sum = 0;
+        for(long elem : cvg) {
+            sum += elem;
+        }
+        for(int iter = 0; iter < cvg.length; iter++) {
+            avgProp[iter] = cvg[iter]/sum;
+        }
+
+        return avgProp;
+    }
+
+    private void printToFiles(List<SAMRecord>[] sampledNewReads, ArtificialPoolContext context) {
+        SAMFileWriter samWrite = context.getSAMFileWriter();
+        String sp = "      ";
+        PrintWriter auxWrite = context.getWriterToAuxiliaryFile();
+        int readGroupInt = 0;
+        for(List<SAMRecord> readGroup : sampledNewReads) {
+            for(SAMRecord read : readGroup) {
+                samWrite.addAlignment(read);
+            }
+            auxWrite.print(context.getAlignmentContext().getLocation().toString() + sp);
+            auxWrite.print(context.genotypeAndConfidenceToString(readGroupInt,sp));
+            readGroupInt++;
+        }
+
+    }
+
+
+}
+

java/src/org/broadinstitute/sting/playground/gatk/walkers/poolseq/ArtificialPoolWalker.java

@@ -54,6 +54,7 @@ public class ArtificialPoolWalker extends LocusWalker<List<SAMRecord>[], SAMFile
     private LinkedList<Integer>[] living_reads;
     private SingleSampleGenotyper ssg;
     private int npeople;
+    //TODO: LOCAL CLASS FOR ALL THIS
     //@param local_genotypes - holds the genotype (A A/ A C/ etc) for each individual. Updates at each locus.
     //@param auxWrite - the writer to the auxiliary file
     //@param readGroupSets : holds the readgroups (identifiers for individuals from each read)
@@ -86,8 +87,6 @@ public class ArtificialPoolWalker extends LocusWalker<List<SAMRecord>[], SAMFile
 
         if(red_prop <= 0) {
             red_prop =  1.0/npeople;
-        } else {
-            // do nothing muhahaha
         }
 
         // initialize the local genotype array
@@ -112,7 +111,7 @@ public class ArtificialPoolWalker extends LocusWalker<List<SAMRecord>[], SAMFile
 
         updateLiving();
         // each time we move to the next locus, remove from the coverage count those reads that ended
-        auxWrite.printf("%s:%s",context.getContig(),context.getPosition());
+        auxWrite.printf("%s:%s",context.getContig(),context.getPosition());    // TODO: PUT IN REDUCE
 
         return getNewReadsAndGenotypesByGroup(tracker, ref, context);
     }
@@ -134,7 +133,6 @@ public class ArtificialPoolWalker extends LocusWalker<List<SAMRecord>[], SAMFile
         List<SAMRecord>[] randomReadsByGroup = drawReadsRandomlyFromReadsByGroup(readsByReadGroup,sought_coverage);
         printToFileAndAuxFile(randomReadsByGroup,sought_coverage,outFile);
         return outFile;
-
     }
 
 

java/src/org/broadinstitute/sting/playground/gatk/walkers/poolseq/CoverageAndPowerWalker.java

@@ -33,14 +33,9 @@ public class CoverageAndPowerWalker extends LocusWalker<Integer, Pair<Long, Long
     @Argument(fullName="lodThreshold", shortName="lt", doc="Threshold for LOD score for calls")
         public double threshold = 3.0;
      
-
-     
     private static final int BOOTSTRAP_ITERATIONS = 300;
 
-
+    public void initialize() {
-    @Override
-    public void initialize()
-    {
 
         if(num_individuals <= 0)
             throw new IllegalArgumentException("Positive nonzero parameter expected for poolSize");
@@ -117,9 +112,7 @@ public class CoverageAndPowerWalker extends LocusWalker<Integer, Pair<Long, Long
         double pow = 0;
 
         if(depth - kaccept < kaccept) {// kaccept > depth/2 - calculate power as P[hits between k and depth]
-
             for(int k = kaccept; k < depth; k++) {
-
                 pow += MathUtils.binomialProbabilityLog(k, depth, snp_prop);
             }
 
@@ -153,14 +146,8 @@ public class CoverageAndPowerWalker extends LocusWalker<Integer, Pair<Long, Long
         if (depth <= 0) {
             result = new Pair(-1,-1);
         } else if (!use_bootstrap) { // object data from command line
-
             result = powerTheoretical(depth, reads, offsets, single_snip_proportion);
-        } else {
+        } else { // otherwise, bootstrapping occurs below
-
-            //
-            // otherwise, bootstrapping occurs below
-            //
-
             int hypothesis_rejections=0;
 
             for(int boot = 0; boot < BOOTSTRAP_ITERATIONS; boot++)
@@ -196,15 +183,7 @@ public class CoverageAndPowerWalker extends LocusWalker<Integer, Pair<Long, Long
 
     public int randomlySelectRead(int depth)
     {
-        double qscore_selector = Math.random();
+        return (int) Math.floor((double)depth * Math.random());
-        int readspositionrandom;
-        for(readspositionrandom = 1; readspositionrandom < ((double)depth * qscore_selector); readspositionrandom ++) {
-            if(readspositionrandom > depth + 1) {
-                throw new RuntimeException("qscore iterator exceeding possible thresholds");
-            }
-        }
-
-        return readspositionrandom - 1;
     }
 
 

java/src/org/broadinstitute/sting/playground/utils/ArtificialPoolContext.java

@@ -0,0 +1,286 @@
+package org.broadinstitute.sting.playground.utils;
+
+import org.broadinstitute.sting.gatk.walkers.genotyper.SingleSampleGenotyper;
+import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
+import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
+import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
+import org.broadinstitute.sting.utils.Pair;
+import org.broadinstitute.sting.utils.StingException;
+import org.broadinstitute.sting.utils.genotype.GenotypeCall;
+
+import java.io.PrintWriter;
+import java.util.Set;
+import java.util.List;
+import java.util.LinkedList;
+import java.util.ArrayList;
+
+import net.sf.samtools.SAMRecord;
+import net.sf.samtools.SAMFileWriter;
+
+/**
+ * Created by IntelliJ IDEA.
+ * User: chartl
+ * Date: Aug 26, 2009
+ * Time: 11:37:42 AM
+ * To change this template use File | Settings | File Templates.
+ */
+public class ArtificialPoolContext {
+    private PrintWriter writerToAuxiliaryFile;
+    private SAMFileWriter writerToSamFile;
+    private SingleSampleGenotyper ssg;
+    private List<Set<String>> readGroupSets;
+    private long[] runningCoverage;
+    private RefMetaDataTracker refTracker;
+    private ReferenceContext refContext;
+    private AlignmentContext aliContext;
+
+    public ArtificialPoolContext() {
+        readGroupSets = null;
+        writerToAuxiliaryFile = null;
+        writerToSamFile = null;
+        ssg = null;
+        refTracker = null;
+        aliContext = null;
+        refContext = null;
+        runningCoverage = null;
+    }
+
+    public ArtificialPoolContext(RefMetaDataTracker tracker, ReferenceContext ref, AlignmentContext context) {
+        refTracker = tracker;
+        refContext = ref;
+        aliContext = context;
+        readGroupSets = null;
+        writerToAuxiliaryFile = null;
+        writerToSamFile=null;
+        ssg = null;
+        runningCoverage = null;
+    }
+
+    public ArtificialPoolContext(PrintWriter pw, SAMFileWriter sw, SingleSampleGenotyper g, List<Set<String>> rgs, long [] runcvg, RefMetaDataTracker rt, ReferenceContext rc, AlignmentContext ac) {
+        writerToAuxiliaryFile = pw;
+        writerToSamFile = sw;
+        ssg = g;
+        readGroupSets = rgs;
+        runningCoverage = runcvg;
+        refTracker = rt;
+        refContext = rc;
+        aliContext = ac;
+    }
+
+    public void setAuxWriter(PrintWriter writer) {
+        writerToAuxiliaryFile = writer;
+    }
+
+    public void setSingleSampleGenotyper(SingleSampleGenotyper typer) {
+        ssg = typer;
+    }
+
+    public void initializeSSG() {
+        ssg.initialize();
+    }
+
+    public void setReadGroupSets(List<Set<String>> rgSets) {
+        readGroupSets = rgSets;
+    }
+
+    public void setRefMetaDataTracker(RefMetaDataTracker tracker) {
+        refTracker = tracker;
+    }
+
+    public void setReferenceContext(ReferenceContext ref) {
+        refContext = ref;
+    }
+
+    public void setAlignmentContext(AlignmentContext context) {
+        aliContext = context;
+    }
+
+    public void setRunningCoverage(long[] estimate) {
+        runningCoverage = estimate;
+    }
+
+    public void setSAMFileWriter(SAMFileWriter writer) {
+        writerToSamFile = writer;
+    }
+
+    public int getTotalNumberOfPeople() {
+        return readGroupSets.size();
+    }
+
+    public RefMetaDataTracker getRefMetaDataTracker() {
+        return refTracker;
+    }
+
+    public ReferenceContext getReferenceContext() {
+        return refContext;
+    }
+
+    public AlignmentContext getAlignmentContext() {
+        return aliContext;
+    }
+
+    public PrintWriter getWriterToAuxiliaryFile() {
+        return writerToAuxiliaryFile;
+    }
+
+    public SingleSampleGenotyper getSingleSampleGenotyper() {
+        return ssg;
+    }
+
+    public List<Set<String>> getReadGroupSets() {
+        return readGroupSets;
+    }
+
+    public long[] getRunningCoverage() {
+        return runningCoverage;
+    }
+
+    public SAMFileWriter getSAMFileWriter() {
+        return writerToSamFile;
+    }
+
+    public List<SAMRecord> getReads() {
+        List<SAMRecord> reads;
+        if(aliContext == null) {
+            reads=null;
+        } else {
+            reads = aliContext.getReads();
+        }
+
+        return reads;
+    }
+
+    public List<Integer> getOffsets() {
+        List<Integer> offsets;
+        if(aliContext == null) {
+            offsets = null;
+        } else {
+            offsets = aliContext.getOffsets();
+        }
+
+        return offsets;
+    }
+
+    public List<SAMRecord> getNewReads() {
+        List<SAMRecord> newReads;
+
+        if(aliContext == null) {
+            newReads = null;
+        } else {
+            newReads = new LinkedList<SAMRecord>();
+            List<SAMRecord> allReads = aliContext.getReads();
+            List<Integer> allOffsets = aliContext.getOffsets();
+
+            for(int iter = 0; iter < allReads.size(); iter++) {
+                if(allOffsets.get(iter) == 0) {
+                    newReads.add(allReads.get(iter));
+                }
+            }
+        }
+
+        return newReads;
+    }
+
+    public Pair<List<SAMRecord>[],List<Integer>[]> splitByGroup(List<SAMRecord> unsplitReads, List<Integer> unsplitOffsets) {
+        List<SAMRecord>[] readsSplitByGroup;
+        List<Integer> [] offsetsSplitByGroup;
+
+        if(unsplitReads != null && readGroupSets != null) {
+            readsSplitByGroup = new ArrayList[this.getTotalNumberOfPeople()];
+            if(unsplitOffsets != null) {
+                offsetsSplitByGroup = new ArrayList[this.getTotalNumberOfPeople()];
+            }
+            else {
+                offsetsSplitByGroup = null;
+            }
+            int listSize = unsplitReads.size();
+            for(int element = 0; element < listSize; element++) {
+                SAMRecord read = unsplitReads.get(element);
+                for(int groupNumber = 0; groupNumber < this.getTotalNumberOfPeople(); groupNumber++) {
+                    if(readGroupSets.get(groupNumber).contains((String) read.getAttribute("RG"))) {
+                        readsSplitByGroup[groupNumber].add(read);
+                        if(offsetsSplitByGroup != null) {
+                            offsetsSplitByGroup[groupNumber].add(unsplitOffsets.get(element));
+                        }
+                        break;
+                    }
+                }
+            }
+        } else {
+            readsSplitByGroup = null;
+            offsetsSplitByGroup = null; // compiler complains without these lines
+        }
+
+        return new Pair(readsSplitByGroup,offsetsSplitByGroup);
+    }
+
+    public List<SAMRecord>[] splitReadsByGroup(List<SAMRecord> unsplitReads) {
+        return (this.splitByGroup(unsplitReads,null)).first;
+    }
+
+
+    // Static methods follow
+
+    public static ArtificialPoolContext mapReduceMerge(ArtificialPoolContext mapContext, ArtificialPoolContext reduceContext) {
+        return new ArtificialPoolContext(reduceContext.getWriterToAuxiliaryFile(),reduceContext.getSAMFileWriter(),
+                reduceContext.getSingleSampleGenotyper(), reduceContext.getReadGroupSets(), reduceContext.getRunningCoverage(),
+                mapContext.getRefMetaDataTracker(),mapContext.getReferenceContext(),mapContext.getAlignmentContext());
+    }
+
+    public static Pair<List<SAMRecord>[],List<Integer>> sampleReadsAndOffsets(List<SAMRecord>[] reads, List<Integer>[] offsets, double[] propEstGlobal) {
+        double[] samplingRate = calculateSamplingRateFromGlobalEstimate(propEstGlobal);
+        List<SAMRecord>[] sampledReads = new ArrayList[reads.length];
+        List<Integer>[] sampledOffsets;
+        if(offsets != null){
+            sampledOffsets = new ArrayList[offsets.length];
+        } else {
+            sampledOffsets = null;
+        }
+
+        for(int group = 0; group < reads.length; group++) {
+            for(int readNumber = 0; readNumber < reads[group].size(); readNumber++) {
+                if(Math.random() < samplingRate[group]) {
+                    sampledReads[group].add(reads[group].get(readNumber));
+                    if(sampledOffsets != null) {
+                        sampledOffsets[group].add(offsets[group].get(readNumber));
+                    }
+                }
+            }
+        }
+
+        return new Pair(sampledReads,sampledOffsets);
+    }
+
+    public String genotypeAndConfidenceToString(int group, String spacer) {
+        GenotypeCall call = this.getGenotypeCall(group);
+        return (call.getGenotypes() + spacer + call.getConfidenceScore().toString());
+    }
+
+    public GenotypeCall getGenotypeCall(int group) {
+        AlignmentContext alicon = this.getAlignmentContext();
+        Pair<List<SAMRecord>[],List<Integer>[]> byGroupSplitPair = this.splitByGroup(alicon.getReads(),alicon.getOffsets());
+        return ssg.map(this.getRefMetaDataTracker(),this.getReferenceContext(),
+                new AlignmentContext(this.getAlignmentContext().getLocation(), byGroupSplitPair.first[group],byGroupSplitPair.second[group]));
+    }
+
+    public static List<SAMRecord>[] sampleReads(List<SAMRecord>[] reads, double[] propEstGlobal) {
+        return (sampleReadsAndOffsets(reads, null, propEstGlobal)).first;
+    }
+
+    public static double[] calculateSamplingRateFromGlobalEstimate(double[] ratios) {
+        double min = ratios[0];
+        for(double ratio : ratios) {
+            if(ratio < min) {
+                min = ratio;
+            }
+        }
+        double[] samplingRate = new double[ratios.length];
+        // now divide by minimum
+        for(int j = 0; j < ratios.length; j++) {
+            samplingRate[j] = ratios[j]/min;
+        }
+
+        return samplingRate;
+    }
+
+}