From 18fc5c8c3e97f7c7074e140530152907c266500c Mon Sep 17 00:00:00 2001
From: flannick <flannick@348d0f76-0448-11de-a6fe-93d51630548a>
Date: Tue, 10 Aug 2010 19:40:17 +0000
Subject: [PATCH] Initial implementation of annotator to compute allele balance
 for each sample

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@4005 348d0f76-0448-11de-a6fe-93d51630548a
---
 .../annotator/AlleleBalanceBySample.java      | 68 +++++++++++++++++++
 1 file changed, 68 insertions(+)
 create mode 100755 java/src/org/broadinstitute/sting/gatk/walkers/annotator/AlleleBalanceBySample.java

diff --git a/java/src/org/broadinstitute/sting/gatk/walkers/annotator/AlleleBalanceBySample.java b/java/src/org/broadinstitute/sting/gatk/walkers/annotator/AlleleBalanceBySample.java
new file mode 100755
index 000000000..6bc9a709b
--- /dev/null
+++ b/java/src/org/broadinstitute/sting/gatk/walkers/annotator/AlleleBalanceBySample.java
@@ -0,0 +1,68 @@
+package org.broadinstitute.sting.gatk.walkers.annotator;
+
+import org.broad.tribble.vcf.VCFFormatHeaderLine;
+import org.broad.tribble.vcf.VCFHeaderLineType;
+import org.broadinstitute.sting.gatk.contexts.*;
+import org.broad.tribble.util.variantcontext.*;
+import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
+import org.broadinstitute.sting.gatk.walkers.annotator.interfaces.*;
+import org.broadinstitute.sting.utils.pileup.*;
+import org.broadinstitute.sting.utils.*;
+
+import java.util.*;
+
+
+public class AlleleBalanceBySample implements GenotypeAnnotation, ExperimentalAnnotation {
+
+    public Map<String, Object> annotate(RefMetaDataTracker tracker, ReferenceContext ref, StratifiedAlignmentContext stratifiedContext, VariantContext vc, Genotype g) {
+				Double ratio = annotateSNP(stratifiedContext, vc, g);
+				if (ratio == null)
+						return null;
+
+        Map<String, Object> map = new HashMap<String, Object>();
+        map.put(getKeyNames().get(0), String.format("%.2f", ratio.doubleValue()));
+				return map;
+
+   }
+
+    private Double annotateSNP(StratifiedAlignmentContext stratifiedContext, VariantContext vc, Genotype g) {
+
+				double ratio = -1;
+
+        if ( !vc.isSNP() ) 
+						return null;
+
+				if ( !vc.isBiallelic() )
+						return null;
+
+        if ( g == null || !g.isCalled() )
+            return null;
+
+				if (!g.isHet())
+						return null;
+
+        Set<Allele> altAlleles = vc.getAlternateAlleles();
+        if ( altAlleles.size() == 0 )
+            return null;
+
+				final String bases = new String(stratifiedContext.getContext(StratifiedAlignmentContext.StratifiedContextType.COMPLETE).getBasePileup().getBases());
+				if ( bases.length() == 0 )
+						return null;
+				char refChr = vc.getReference().toString().charAt(0);
+				char altChr = vc.getAlternateAllele(0).toString().charAt(0);
+
+				int refCount = MathUtils.countOccurrences(refChr, bases);
+				int altCount = MathUtils.countOccurrences(altChr, bases);
+
+				// sanity check
+				if ( refCount + altCount == 0 )
+						return null;
+
+				ratio = ((double)refCount / (double)(refCount + altCount));
+				return ratio;
+    }
+
+    public List<String> getKeyNames() { return Arrays.asList("AB"); }
+
+    public List<VCFFormatHeaderLine> getDescriptions() { return Arrays.asList(new VCFFormatHeaderLine(getKeyNames().get(0), -1, VCFHeaderLineType.Float, "Allele balance for each het genotype")); }
+}
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