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Fixed bug with mrnaCoord field - made it count exon positions only, rather than introns & exons 

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@3642 348d0f76-0448-11de-a6fe-93d51630548a
This commit is contained in:
weisburd 2010-06-25 19:53:32 +00:00
parent ce27ed0d60
commit 147ba68441
1 changed files with 8 additions and 6 deletions
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14
java/src/org/broadinstitute/sting/playground/gatk/walkers/annotator/TranscriptToInfo.java
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@ -362,7 +362,6 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
{
final String geneNamePortionOfSortKey = computeGeneNamePortionOfSortKey(parsedTranscriptRod.geneNames);
final TranscriptTableRecord collisionRecord = keyChecker.get( geneNamePortionOfSortKey );
System.err.println("Checking key: " + geneNamePortionOfSortKey + " - got record: " + collisionRecord);
if(collisionRecord != null /* && new Interval( - don't allow collitions even if positions are different.
collisionRecord.txChrom,
(int) collisionRecord.txStart,
@ -482,6 +481,7 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
int frame = 0; //the frame of the current position
int txOffset_from5 = 1; //goes from txStart 5' to txEnd 3' for both + and - strand
int utr5Count_from5 = 0;
int mrnaCoord_from5 = 1; //goes from txStart 5' to txEnd 3' for both + and - strand, but only counts bases within exons.
char[] utr5NucBuffer_5to3 = null; //used to find uORFs - size = 5 because to hold the 3 codons that overlap any given position: [-2,-1,0], [-1,0,1], and [0,1,2]
int codonCount_from5 = 1; //goes from cdsStart 5' to cdsEnd 3' for both + and - strand - counts the number of codons - 1-based
@ -686,10 +686,12 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
outputLineFields.put(OUTPUT_HAPLOTYPE_STRAND, positiveStrand ? "+" : "-");
for(int i = 0; i < GENE_NAME_COLUMNS.length; i++) {
outputLineFields.put(GENE_NAME_COLUMNS[i], parsedTranscriptRod.geneNames[i] );
}
}
outputLineFields.put(OUTPUT_POSITION_TYPE, positionType.toString() );
outputLineFields.put(OUTPUT_MRNA_COORD, Integer.toString(txOffset_from5) );
if(isWithinExon) {
outputLineFields.put(OUTPUT_MRNA_COORD, Integer.toString(mrnaCoord_from5) );
}
outputLineFields.put(OUTPUT_SPLICE_DISTANCE, Integer.toString(distanceToNearestSpliceSite) );
//compute OUTPUT_SPLICE_INFO
@ -832,6 +834,9 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
{
//increment coords
txOffset_from5++;
if(isWithinExon) {
mrnaCoord_from5++;
}
if(positionType == PositionType.utr5) {
utr5Count_from5++;
@ -963,7 +968,6 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
return;
}
//move the fully merged temp file to the output file.
logger.info("Writing " + result.size() + " lines to: " + outputFilename + ". Average of " + (totalPositionsCounter == 0 ? 0 : (10*result.size()/totalPositionsCounter)/10.0f) + " lines per genomic position.");
BufferedWriter fileWriter = null;
try {
@ -988,8 +992,6 @@ public class TranscriptToInfo extends RodWalker<TreeMap<String, String>, TreeMap
logger.info("Protein-coding transcripts (eg. with a CDS region) that don't start with Methionine or end in a stop codon: " + transcriptsThatDontStartWithMethionineOrEndWithStopCodonCounter + " transcripts out of "+ transcriptsProcessedCounter + " total (" + ( transcriptsProcessedCounter == 0 ? 0 : (100*transcriptsThatDontStartWithMethionineOrEndWithStopCodonCounter)/transcriptsProcessedCounter) + "%)");
logger.info("Protein-coding transcripts (eg. with a CDS region) that don't start with Methionine: " + transcriptsThatDontStartWithMethionineCounter + " transcripts out of "+ transcriptsProcessedCounter + " total (" + ( transcriptsProcessedCounter == 0 ? 0 : (100*transcriptsThatDontStartWithMethionineCounter)/transcriptsProcessedCounter) + "%)");
logger.info("Protein-coding transcripts (eg. with a CDS region) that don't end in a stop codon: " + transcriptsThatDontEndWithStopCodonCounter + " transcripts out of "+ transcriptsProcessedCounter + " total (" + ( transcriptsProcessedCounter == 0 ? 0 : (100*transcriptsThatDontEndWithStopCodonCounter)/transcriptsProcessedCounter) + "%)");
logger.info("Deleting temp files..");
}