a first, simplest version of a walker that filters out reads based on user-specified criteria and writes remaining reads into a new bam file

git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@467 348d0f76-0448-11de-a6fe-93d51630548a
This commit is contained in:
asivache 2009-04-17 18:51:39 +00:00
parent 1660379753
commit 03ec3452f2
1 changed files with 48 additions and 0 deletions

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package org.broadinstitute.sting.playground.gatk.walkers;
import java.io.File;
import net.sf.samtools.SAMFileWriter;
import net.sf.samtools.SAMFileWriterFactory;
import net.sf.samtools.SAMRecord;
import org.broadinstitute.sting.gatk.LocusContext;
import org.broadinstitute.sting.gatk.walkers.ReadWalker;
import org.broadinstitute.sting.utils.cmdLine.Argument;
public class ReadFilterWalker extends ReadWalker<Integer,Integer> {
@Argument(fullName="output_file", shortName="O",doc="SAM or BAM file to write filtered reads into (will be overwritten if exists)",required=true ) public String output;
@Argument(fullName="max_read_length",doc="Discard reads with length greater than the specified value",required=false) public Integer max_len;
private SAMFileWriter writer = null;
@Override
public boolean filter(LocusContext context, SAMRecord read) {
if ( read.getReadLength() > max_len ) return false;
return true;
}
@Override
public Integer map(LocusContext context, SAMRecord read) {
if ( writer == null ) writer = new SAMFileWriterFactory().makeSAMOrBAMWriter(read.getHeader(), true, new File(output));
writer.addAlignment(read);
return 1;
}
@Override
public Integer reduce(Integer value, Integer sum) {
return sum+value;
}
@Override
public Integer reduceInit() {
return 0;
}
public void onTraversalDone(Integer nReads) {
super.onTraversalDone(nReads);
out.println(nReads +" reads passed the filter and were written into output file "+output);
}
}