Number of MQ0 reads in each sample (format field)
git-svn-id: file:///humgen/gsa-scr1/gsa-engineering/svn_contents/trunk@5229 348d0f76-0448-11de-a6fe-93d51630548a
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/*
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* Copyright (c) 2010 The Broad Institute
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*
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* Permission is hereby granted, free of charge, to any person
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* obtaining a copy of this software and associated documentation
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* files (the "Software"), to deal in the Software without
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* restriction, including without limitation the rights to use,
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* copy, modify, merge, publish, distribute, sublicense, and/or sell
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* copies of the Software, and to permit persons to whom the
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* Software is furnished to do so, subject to the following
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* conditions:
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*
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* The above copyright notice and this permission notice shall be
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* included in all copies or substantial portions of the Software.
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*
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* THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND,
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* EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES
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* OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND
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* NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT
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* HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY,
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* WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
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* FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR
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* OTHER DEALINGS IN THE SOFTWARE.
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*/
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package org.broadinstitute.sting.gatk.walkers.annotator;
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import org.broadinstitute.sting.gatk.walkers.annotator.interfaces.GenotypeAnnotation;
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import org.broadinstitute.sting.gatk.refdata.RefMetaDataTracker;
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import org.broadinstitute.sting.gatk.contexts.ReferenceContext;
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import org.broadinstitute.sting.gatk.contexts.StratifiedAlignmentContext;
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import org.broadinstitute.sting.gatk.contexts.AlignmentContext;
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import org.broadinstitute.sting.utils.pileup.ReadBackedPileup;
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import org.broadinstitute.sting.utils.pileup.PileupElement;
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import org.broad.tribble.util.variantcontext.VariantContext;
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import org.broad.tribble.util.variantcontext.Genotype;
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import org.broad.tribble.vcf.VCFConstants;
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import org.broad.tribble.vcf.VCFHeaderLineType;
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import org.broad.tribble.vcf.VCFFormatHeaderLine;
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import java.util.Map;
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import java.util.HashMap;
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import java.util.List;
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import java.util.Arrays;
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/**
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* Created by IntelliJ IDEA.
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* User: asivache
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* Date: Feb 4, 2011
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* Time: 6:46:25 PM
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* To change this template use File | Settings | File Templates.
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*/
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public class MappingQualityZeroBySample implements GenotypeAnnotation {
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public Map<String, Object> annotate(RefMetaDataTracker tracker, ReferenceContext ref,
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StratifiedAlignmentContext stratifiedContext, VariantContext vc, Genotype g) {
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if ( g == null || !g.isCalled() )
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return null;
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int mq0 = 0;
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AlignmentContext context = stratifiedContext.getContext(StratifiedAlignmentContext.StratifiedContextType.COMPLETE);
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ReadBackedPileup pileup = null;
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if (vc.isIndel() && context.hasExtendedEventPileup())
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pileup = context.getExtendedEventPileup();
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else if (context.hasBasePileup())
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pileup = context.getBasePileup();
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else return null;
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if (pileup != null) {
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for (PileupElement p : pileup ) {
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if ( p.getMappingQual() == 0 )
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mq0++;
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}
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}
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Map<String, Object> map = new HashMap<String, Object>();
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map.put(getKeyNames().get(0), String.format("%d", mq0));
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return map;
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}
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public List<String> getKeyNames() { return Arrays.asList(VCFConstants.MAPPING_QUALITY_ZERO_KEY); }
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public List<VCFFormatHeaderLine> getDescriptions() { return Arrays.asList(
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new VCFFormatHeaderLine(getKeyNames().get(0), 1,
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VCFHeaderLineType.Integer, "Number of Mapping Quality Zero Reads per sample")); }
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}
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