gatk-3.8/scala/qscript/playground/recalibrate.scala

import org.broadinstitute.sting.queue.extensions.gatk._
import org.broadinstitute.sting.queue.extensions.samtools.SamtoolsIndexFunction
import org.broadinstitute.sting.queue.QScript
import org.apache.commons.io.FilenameUtils;

class recalibrate extends QScript {
  @Input(doc="bamIn", shortName="I")
  var bamIns: List[File] = Nil
  
  @Argument(doc="scatter")
  var scatter = false

  @Argument(doc="gatk jar file")
  var gatkJarFile: File = _

  @Argument(doc="Assume initial count covariates has completed", required=false)
  var skipInitialCountCovariates: Boolean = false

  @Argument(doc="", required=false)
  var skipUQUpdateArg: Boolean = false

  @Argument(shortName = "R", doc="ref")
  var referenceFile: File = _

  trait UNIVERSAL_GATK_ARGS extends CommandLineGATK { logging_level = "INFO"; jarFile = gatkJarFile; reference_sequence = referenceFile;  }

def script = {
    for (bamIn <- bamIns) {
      val root = bamIn.getPath()
      val bamRoot = FilenameUtils.removeExtension(root);
      val recalData = new File(bamRoot + ".recal_data.csv")
      val recalBam = new File(bamRoot + ".recal.bam")
      val recalRecalData = new File(bamRoot + ".recal.recal_data.csv")
      if ( ! skipInitialCountCovariates )
          add(new CountCovariates(bamIn, recalData) { useOriginalQualities = true } )
      val tableRecal = new TableRecalibrate(bamIn, recalData, recalBam) { useOriginalQualities = true }
      if ( scatter ) {
          tableRecal.intervals = List(new File("/humgen/gsa-hpprojects/GATK/data/chromosomes.hg18.interval_list"))
      	  tableRecal.scatterCount = 25
      }
      add(tableRecal)
      add(new Index(recalBam))
      add(new CountCovariates(recalBam, recalRecalData) { num_threads = 4 })
      add(new AnalyzeCovariates(recalData, new File(recalData.getPath() + ".analyzeCovariates")))
      add(new AnalyzeCovariates(recalRecalData, new File(recalRecalData.getPath() + ".analyzeCovariates")))
    }
}

def bai(bam: File) = new File(bam + ".bai")

class Index(bamIn: File) extends SamtoolsIndexFunction {
    bamFile = bamIn
}

class CountCovariates(bamIn: File, recalDataIn: File) extends org.broadinstitute.sting.queue.extensions.gatk.CountCovariates with UNIVERSAL_GATK_ARGS {
    this.jarFile = gatkJarFile
    this.input_file :+= bamIn
    this.recal_file = recalDataIn
    this.DBSNP = new File("/humgen/gsa-hpprojects/GATK/data/dbsnp_129_hg18.rod")
    this.logging_level = "INFO"
    this.covariate ++= List("ReadGroupCovariate", "QualityScoreCovariate", "CycleCovariate", "DinucCovariate")
    this.memoryLimit = 3
    
    override def dotString = "CountCovariates: %s [args %s]".format(bamIn.getName, if (this.num_threads.isDefined) "-nt " + this.num_threads else "")
}

class TableRecalibrate(bamInArg: File, recalDataIn: File, bamOutArg: File) extends org.broadinstitute.sting.queue.extensions.gatk.TableRecalibration with UNIVERSAL_GATK_ARGS {
    this.jarFile = gatkJarFile
    this.input_file :+= bamInArg
    this.recal_file = recalDataIn
    this.out = bamOutArg
    this.logging_level = "INFO"
    this.memoryLimit = 2
    this.skipUQUpdate = skipUQUpdateArg

    override def dotString = "TableRecalibrate: %s => %s".format(bamInArg.getName, bamOutArg.getName, if (this.useOriginalQualities) " -OQ" else "")
}

class AnalyzeCovariates(recalDataIn: File, outputDir: File) extends  org.broadinstitute.sting.queue.extensions.gatk.AnalyzeCovariates {
    this.jarFile = new File("/home/radon01/depristo/dev/GenomeAnalysisTK/trunk/dist/AnalyzeCovariates.jar")
    this.recal_file = recalDataIn
    this.output_dir = outputDir.toString
    this.path_to_resources = "/home/radon01/depristo/dev/GenomeAnalysisTK/trunk/R/"
    this.ignoreQ = 5
    this.path_to_Rscript = "/broad/software/free/Linux/redhat_5_x86_64/pkgs/r_2.7.2/bin/Rscript"
    this.memoryLimit = 2

    override def dotString = "AnalyzeCovariates: %s".format(recalDataIn.getName)
}
}