29 lines
860 B
Bash
Executable File
29 lines
860 B
Bash
Executable File
#!/bin/bash
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if [ $# -lt 2 ]; then
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echo "Usage: $0 <prefix> <gene>"
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exit 1
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fi
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root=`dirname $0`
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pre=$1.$2
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len=`$root/seqtk comp $pre.fq | awk '{++x;y+=$2}END{printf("%.0f\n", y/x)}'`
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# de novo assembly
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$root/fermi2.pl unitig -t2 -l$len -p $pre.tmp $pre.fq > $pre.tmp.mak
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make -f $pre.tmp.mak
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# get contigs overlapping HLA exons
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$root/bwa mem -B1 -O1 -E1 $root/HLA-ALT.fa $pre.tmp.mag.gz | grep -v ^@ | sort -k3,3 -k4,4n | bgzip > $pre.tmp.alt.gz
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$root/tabix -Bpsam $pre.tmp.alt.gz $root/HLA-approx.anno | cut -f1 | sort | uniq | $root/seqtk subseq $pre.tmp.mag.gz - | gzip -1 > $pre.tmp.fq.gz
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# map HLA exons to de novo contigs
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$root/bwa index -p $pre.tmp $pre.tmp.fq.gz
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$root/bwa mem -aD.1 -t2 $pre.tmp $root/hla.fa | egrep "^(@|$2)" > $pre.tmp.sam
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# type
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$root/k8 $root/bwa-typeHLA.js $pre.tmp.sam > $pre.gt
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# delete temporary files
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rm -f $pre.tmp.*
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