#include #include #include #include "bwa.h" #include "bwt.h" #include "bwtgap.h" #include "bntseq.h" #ifndef kroundup32 #define kroundup32(x) (--(x), (x)|=(x)>>1, (x)|=(x)>>2, (x)|=(x)>>4, (x)|=(x)>>8, (x)|=(x)>>16, ++(x)) #endif extern unsigned char nst_nt4_table[256]; bwa_opt_t bwa_def_opt = { 11, 4, -1, 1, 6, 32, 2, 0.04 }; struct bwa_idx_t { bwt_t *bwt; bntseq_t *bns; uint8_t *pac; }; struct bwa_aux_t { int max_buf; gap_stack_t *stack; gap_opt_t *opt; int *diff_tab; uint8_t *buf; }; bwa_idx_t *bwa_idx_load(const char *prefix) { bwa_idx_t *p; int l; char *str; l = strlen(prefix); p = calloc(1, sizeof(bwa_idx_t)); str = malloc(l + 10); strcpy(str, prefix); p->bns = bns_restore(str); strcpy(str + l, ".bwt"); p->bwt = bwt_restore_bwt(str); str[l] = 0; strcpy(str + l, ".sa"); bwt_restore_sa(str, p->bwt); free(str); p->pac = calloc(p->bns->l_pac/4+1, 1); fread(p->pac, 1, p->bns->l_pac/4+1, p->bns->fp_pac); fclose(p->bns->fp_pac); p->bns->fp_pac = 0; return p; } void bwa_idx_destroy(bwa_idx_t *p) { bns_destroy(p->bns); bwt_destroy(p->bwt); free(p->pac); free(p); } bwa_aux_t *bwa_aux_init(const bwa_opt_t *opt, int max_score) { extern gap_opt_t *gap_init_opt(void); extern int bwa_cal_maxdiff(int l, double err, double thres); int i; bwa_aux_t *p; p = malloc(sizeof(bwa_aux_t)); p->stack = gap_init_stack2(max_score); p->opt = gap_init_opt(); p->opt->s_gapo = opt->s_gapo; p->opt->s_gape = opt->s_gape; p->opt->max_diff = opt->max_diff; p->opt->max_gapo = opt->max_gapo; p->opt->max_gape = opt->max_gape; p->opt->seed_len = opt->seed_len; p->opt->max_seed_diff = opt->max_seed_diff; p->opt->fnr = opt->fnr; p->diff_tab = calloc(BWA_MAX_QUERY_LEN, sizeof(int)); for (i = 1; i < BWA_MAX_QUERY_LEN; ++i) p->diff_tab[i] = bwa_cal_maxdiff(i, BWA_AVG_ERR, opt->fnr); return p; } void bwa_aux_destroy(bwa_aux_t *p) { gap_destroy_stack(p->stack); free(p->diff_tab); free(p->opt); free(p); } bwa_alnpre_t *bwa_aln_pre(const bwa_idx_t *idx, bwa_aux_t *aux, const char *seq, int *n_aln) { extern int bwt_cal_width(const bwt_t *bwt, int len, const ubyte_t *str, bwt_width_t *width); extern void seq_reverse(int len, uint8_t *seq, int is_comp); int i, seq_len, buf_len; bwt_width_t *w, *seed_w; uint8_t *s; gap_opt_t opt2 = *aux->opt; seq_len = strlen(seq); // estimate the buffer length buf_len = (aux->opt->seed_len + seq_len + 1) * sizeof(bwt_width_t) + seq_len; if (buf_len > aux->max_buf) { aux->max_buf = buf_len; kroundup32(aux->max_buf); aux->buf = realloc(aux->buf, aux->max_buf); } memset(aux->buf, 0, buf_len); seed_w = (bwt_width_t*)aux->buf; w = seed_w + aux->opt->seed_len; s = (uint8_t*)(w + seq_len + 1); if (opt2.fnr > 0.) opt2.max_diff = aux->diff_tab[seq_len]; // copy the sequence for (i = 0; i < seq_len; ++i) s[i] = nst_nt4_table[(int)seq[i]]; seq_reverse(seq_len, s, 0); // mapping bwt_cal_width(idx->bwt, seq_len, s, w); if (opt2.seed_len >= seq_len) opt2.seed_len = 0x7fffffff; if (seq_len > aux->opt->seed_len) bwt_cal_width(idx->bwt, aux->opt->seed_len, s + (seq_len - aux->opt->seed_len), seed_w); for (i = 0; i < seq_len; ++i) // complement; I forgot why... s[i] = s[i] > 3? 4 : 3 - s[i]; return (bwa_alnpre_t*)bwt_match_gap(idx->bwt, seq_len, s, w, seq_len <= aux->opt->seed_len? 0 : seed_w, &opt2, n_aln, aux->stack); }