#include #include #include #include #include "bntseq.h" #include "bwt.h" #include "kvec.h" #include "kseq.h" KSEQ_INIT(gzFile, gzread) extern unsigned char nst_nt4_table[256]; int main_fastmap(int argc, char *argv[]) { int c, i, min_iwidth = 20, min_len = 17; kseq_t *seq; bwtint_t k; gzFile fp; bwt_t *bwt; bntseq_t *bns; bwtintv_v a[3], mem, *tvec[3]; while ((c = getopt(argc, argv, "w:l:")) >= 0) { switch (c) { case 'w': min_iwidth = atoi(optarg); break; case 'l': min_len = atoi(optarg); break; } } if (optind + 1 >= argc) { fprintf(stderr, "Usage: bwa fastmap [-l minLen=%d] [-w maxSaSize=%d] \n", min_len, min_iwidth); return 1; } fp = gzopen(argv[optind + 1], "r"); seq = kseq_init(fp); { // load the packed sequences, BWT and SA char *tmp = calloc(strlen(argv[optind]) + 5, 1); strcat(strcpy(tmp, argv[optind]), ".bwt"); bwt = bwt_restore_bwt(tmp); strcat(strcpy(tmp, argv[optind]), ".sa"); bwt_restore_sa(tmp, bwt); free(tmp); bns = bns_restore(argv[optind]); } for (i = 0; i < 3; ++i) { // initiate the temporary array kv_init(a[i]); tvec[i] = &a[i]; } kv_init(mem); while (kseq_read(seq) >= 0) { for (i = 0; i < seq->seq.l; ++i) seq->seq.s[i] = nst_nt4_table[(int)seq->seq.s[i]]; bwt_smem(bwt, seq->seq.l, (uint8_t*)seq->seq.s, &mem, tvec); printf("SQ\t%s\t%ld\n", seq->name.s, seq->seq.l); for (i = 0; i < mem.n; ++i) { bwtintv_t *p = &mem.a[i]; if ((uint32_t)p->info - (p->info>>32) < min_len) continue; printf("EM\t%d\t%d\t%ld", (uint32_t)(p->info>>32), (uint32_t)p->info, (long)p->x[2]); if (p->x[2] <= min_iwidth) { for (k = 0; k < p->x[2]; ++k) { bwtint_t pos; int len, is_rev, ref_id; len = (uint32_t)p->info - (p->info>>32); pos = bns_depos(bns, bwt_sa(bwt, p->x[0] + k), &is_rev); if (is_rev) pos -= len - 1; bns_cnt_ambi(bns, pos, len, &ref_id); printf("\t%s:%c%ld", bns->anns[ref_id].name, "+-"[is_rev], (long)(pos - bns->anns[ref_id].offset) + 1); } } putchar('\n'); } puts("//"); } free(mem.a); for (i = 0; i < 3; ++i) free(a[i].a); bns_destroy(bns); bwt_destroy(bwt); kseq_destroy(seq); gzclose(fp); return 0; }