#include #include #include #include #include "bntseq.h" #include "bwt.h" #include "bwamem.h" #include "kvec.h" #include "bseq.h" #include "kseq.h" KSEQ_DECLARE(gzFile) extern unsigned char nst_nt4_table[256]; int main_mem(int argc, char *argv[]) { mem_opt_t *opt; bwt_t *bwt; bntseq_t *bns; int c, n; gzFile fp, fp2 = 0; kseq_t *ks, *ks2 = 0; uint8_t *pac = 0; bseq1_t *seqs; opt = mem_opt_init(); while ((c = getopt(argc, argv, "k:c:v:s:")) >= 0) { if (c == 'k') opt->min_seed_len = atoi(optarg); else if (c == 'c') opt->max_occ = atoi(optarg); else if (c == 'v') mem_verbose = atoi(optarg); else if (c == 's') opt->split_width = atoi(optarg); } if (optind + 1 >= argc) { fprintf(stderr, "\n"); fprintf(stderr, "Usage: bwa mem [options] \n\n"); fprintf(stderr, "Options: -k INT minimum seed length [%d]\n", opt->min_seed_len); fprintf(stderr, " -c INT skip seeds with more than INT occurrences [%d]\n", opt->max_occ); fprintf(stderr, " -s INT look for internal seeds inside a seed with less than INT occ [%d]\n", opt->split_width); fprintf(stderr, " -v INT verbose level [%d]\n", mem_verbose); fprintf(stderr, "\n"); free(opt); return 1; } mem_fill_scmat(opt->a, opt->b, opt->mat); { // load the packed sequences, BWT and SA char *tmp = calloc(strlen(argv[optind]) + 5, 1); strcat(strcpy(tmp, argv[optind]), ".bwt"); bwt = bwt_restore_bwt(tmp); strcat(strcpy(tmp, argv[optind]), ".sa"); bwt_restore_sa(tmp, bwt); free(tmp); bns = bns_restore(argv[optind]); pac = calloc(bns->l_pac/4+1, 1); fread(pac, 1, bns->l_pac/4+1, bns->fp_pac); } fp = strcmp(argv[optind + 1], "-")? gzopen(argv[optind + 1], "r") : gzdopen(fileno(stdin), "r"); ks = kseq_init(fp); if (optind + 2 < argc) { fp2 = gzopen(argv[optind + 2], "r"); ks2 = kseq_init(fp); opt->is_pe = 1; } while ((seqs = bseq_read(opt->n_threads * opt->chunk_size, &n, ks, ks2)) != 0) { mem_process_seqs(opt, bwt, bns, pac, n, seqs); free(seqs); } free(opt); free(pac); bns_destroy(bns); bwt_destroy(bwt); kseq_destroy(ks); gzclose(fp); if (ks2) { kseq_destroy(ks2); gzclose(fp2); } return 0; } int main_fastmap(int argc, char *argv[]) { int c, i, min_iwidth = 20, min_len = 17, print_seq = 0, split_width = 0; kseq_t *seq; bwtint_t k; gzFile fp; bwt_t *bwt; bntseq_t *bns; smem_i *itr; const bwtintv_v *a; while ((c = getopt(argc, argv, "w:l:ps:")) >= 0) { switch (c) { case 's': split_width = atoi(optarg); break; case 'p': print_seq = 1; break; case 'w': min_iwidth = atoi(optarg); break; case 'l': min_len = atoi(optarg); break; } } if (optind + 1 >= argc) { fprintf(stderr, "Usage: bwa fastmap [-p] [-s splitWidth=%d] [-l minLen=%d] [-w maxSaSize=%d] \n", split_width, min_len, min_iwidth); return 1; } fp = gzopen(argv[optind + 1], "r"); seq = kseq_init(fp); { // load the packed sequences, BWT and SA char *tmp = calloc(strlen(argv[optind]) + 5, 1); strcat(strcpy(tmp, argv[optind]), ".bwt"); bwt = bwt_restore_bwt(tmp); strcat(strcpy(tmp, argv[optind]), ".sa"); bwt_restore_sa(tmp, bwt); free(tmp); bns = bns_restore(argv[optind]); } itr = smem_itr_init(bwt); while (kseq_read(seq) >= 0) { printf("SQ\t%s\t%ld", seq->name.s, seq->seq.l); if (print_seq) { putchar('\t'); puts(seq->seq.s); } else putchar('\n'); for (i = 0; i < seq->seq.l; ++i) seq->seq.s[i] = nst_nt4_table[(int)seq->seq.s[i]]; smem_set_query(itr, seq->seq.l, (uint8_t*)seq->seq.s); while ((a = smem_next(itr, min_len<<1, split_width)) != 0) { for (i = 0; i < a->n; ++i) { bwtintv_t *p = &a->a[i]; if ((uint32_t)p->info - (p->info>>32) < min_len) continue; printf("EM\t%d\t%d\t%ld", (uint32_t)(p->info>>32), (uint32_t)p->info, (long)p->x[2]); if (p->x[2] <= min_iwidth) { for (k = 0; k < p->x[2]; ++k) { bwtint_t pos; int len, is_rev, ref_id; len = (uint32_t)p->info - (p->info>>32); pos = bns_depos(bns, bwt_sa(bwt, p->x[0] + k), &is_rev); if (is_rev) pos -= len - 1; bns_cnt_ambi(bns, pos, len, &ref_id); printf("\t%s:%c%ld", bns->anns[ref_id].name, "+-"[is_rev], (long)(pos - bns->anns[ref_id].offset) + 1); } } else fputs("\t*", stdout); putchar('\n'); } } puts("//"); } smem_itr_destroy(itr); bns_destroy(bns); bwt_destroy(bwt); kseq_destroy(seq); gzclose(fp); return 0; }