#include "bwa.h" #include "bwamem.h" #include "bntseq.h" #include "kstring.h" /*************************** * SMEM iterator interface * ***************************/ struct __smem_i { const bwt_t *bwt; const uint8_t *query; int start, len; bwtintv_v *matches; // matches; to be returned by smem_next() bwtintv_v *sub; // sub-matches inside the longest match; temporary bwtintv_v *tmpvec[2]; // temporary arrays }; smem_i *smem_itr_init(const bwt_t *bwt) { smem_i *itr; itr = calloc(1, sizeof(smem_i)); itr->bwt = bwt; itr->tmpvec[0] = calloc(1, sizeof(bwtintv_v)); itr->tmpvec[1] = calloc(1, sizeof(bwtintv_v)); itr->matches = calloc(1, sizeof(bwtintv_v)); itr->sub = calloc(1, sizeof(bwtintv_v)); return itr; } void smem_itr_destroy(smem_i *itr) { free(itr->tmpvec[0]->a); free(itr->tmpvec[0]); free(itr->tmpvec[1]->a); free(itr->tmpvec[1]); free(itr->matches->a); free(itr->matches); free(itr->sub->a); free(itr->sub); free(itr); } void smem_set_query(smem_i *itr, int len, const uint8_t *query) { itr->query = query; itr->start = 0; itr->len = len; } const bwtintv_v *smem_next(smem_i *itr) { int i, max, max_i, ori_start; itr->tmpvec[0]->n = itr->tmpvec[1]->n = itr->matches->n = itr->sub->n = 0; if (itr->start >= itr->len || itr->start < 0) return 0; while (itr->start < itr->len && itr->query[itr->start] > 3) ++itr->start; // skip ambiguous bases if (itr->start == itr->len) return 0; ori_start = itr->start; itr->start = bwt_smem1(itr->bwt, itr->len, itr->query, ori_start, 1, itr->matches, itr->tmpvec); // search for SMEM if (itr->matches->n == 0) return itr->matches; // well, in theory, we should never come here for (i = max = 0, max_i = 0; i < itr->matches->n; ++i) { // look for the longest match bwtintv_t *p = &itr->matches->a[i]; int len = (uint32_t)p->info - (p->info>>32); if (max < len) max = len, max_i = i; } return itr->matches; } /*********************** *** Extra functions *** ***********************/ mem_alnreg_v mem_align1(const mem_opt_t *opt, const bwt_t *bwt, const bntseq_t *bns, const uint8_t *pac, int l_seq, const char *seq_) { // the difference from mem_align1_core() is that this routine: 1) calls mem_mark_primary_se(); 2) does not modify the input sequence extern mem_alnreg_v mem_align1_core(const mem_opt_t *opt, const bwt_t *bwt, const bntseq_t *bns, const uint8_t *pac, int l_seq, char *seq); extern void mem_mark_primary_se(const mem_opt_t *opt, int n, mem_alnreg_t *a, int64_t id); mem_alnreg_v ar; char *seq; seq = malloc(l_seq); memcpy(seq, seq_, l_seq); // makes a copy of seq_ ar = mem_align1_core(opt, bwt, bns, pac, l_seq, seq); mem_mark_primary_se(opt, ar.n, ar.a, lrand48()); free(seq); return ar; } void mem_reg2ovlp(const mem_opt_t *opt, const bntseq_t *bns, const uint8_t *pac, bseq1_t *s, mem_alnreg_v *a) { int i; kstring_t str = {0,0,0}; for (i = 0; i < a->n; ++i) { const mem_alnreg_t *p = &a->a[i]; int is_rev, rid, qb = p->qb, qe = p->qe; int64_t pos, rb = p->rb, re = p->re; if (bwa_fix_xref2(opt->mat, opt->o_del, opt->e_del, opt->o_ins, opt->e_ins, opt->w, bns, pac, (uint8_t*)s->seq, &qb, &qe, &rb, &re) < 0) { if (bwa_verbose >= 2) fprintf(stderr, "[W::%s] A cross-chr hit of read '%s' has been dropped.\n", __func__, s->name); continue; } pos = bns_depos(bns, rb < bns->l_pac? rb : re - 1, &is_rev); rid = bns_pos2rid(bns, pos); pos -= bns->anns[rid].offset; kputs(s->name, &str); kputc('\t', &str); kputw(s->l_seq, &str); kputc('\t', &str); if (is_rev) qb ^= qe, qe ^= qb, qb ^= qe; // swap kputw(qb, &str); kputc('\t', &str); kputw(qe, &str); kputc('\t', &str); kputs(bns->anns[rid].name, &str); kputc('\t', &str); kputw(bns->anns[rid].len, &str); kputc('\t', &str); kputw(pos, &str); kputc('\t', &str); kputw(pos + (re - rb), &str); kputc('\t', &str); kputw(p->truesc, &str); kputc('\n', &str); } s->sam = str.s; }