Commit Graph

10 Commits (32f2d60a2e6406c3114a7bd7f6a11f7413dfcb0a)

Author SHA1 Message Date
Heng Li 5a0b32bfd2 updated to the latest kseq.h 2013-02-06 14:38:40 -05:00
Heng Li 717959e819 fixed a minor issue in trimming 2011-11-24 10:39:41 -05:00
Heng Li dec584d50b fixed a long-existing bug in trimming 2011-11-23 23:11:50 -05:00
Roel Kluin db59a605d1 Remove sequences marked to be filtered by Casava-1.8 with bwa aln -Y
In Casava 1.8 the fastq output changed. e.g.

@EAS139:136:FC706VJ:2:5:1000:12850 1:Y:18:ATCACG
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
+
BBBBCCCC?<A?BC?7@@???????DBBA@@@@A@@

The part after the space, treated as comment by bwa, contains the fields:
<read number>:<is filtered>:<control number>:<barcode sequence>

With `Y' Casava indicates that a sequence should be filtered. This patch
enables bwa, with an -Y flag, to filter these sequences.

Signed-off-by: Roel Kluin <roel.kluin@gmail.com>
2011-07-10 17:04:06 +02:00
Roel Kluin 8f115a8e00 Revert "In Casava 1.8 the fastq output changed, the name had a space which bwa"
This reverts commit 36cd4f9882.

The comment shouldn't be included in the sequence name.
2011-07-10 16:40:42 +02:00
RoelKluin 36cd4f9882 In Casava 1.8 the fastq output changed, the name had a space which bwa
wasn't parsing correctly. This patch fixes that and enables bwa to filter
sequences marked by Casava, removing this tag from the output.

Signed-off-by: RoelKluin <roel.kluin@gmail.com>
2011-07-08 16:49:09 +02:00
Heng Li a74523a68d increase maximum barcode length limit to 63bp 2011-06-09 17:17:13 -04:00
Heng Li 51d354cd28 Added barcode support 2011-01-15 15:35:39 -05:00
Heng Li 10721ca602 Added an option to accept Illumina 1.3+ fastq 2011-01-15 14:07:08 -05:00
Heng Li 007c3eb75d Imported from my local bwa repository, the master repository. 2011-01-13 20:52:12 -05:00