updated maual page
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@ -91,6 +91,10 @@ settings:
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bwa aln ref.fa read1.fq > read1.sai; bwa aln ref.fa read2.fq > read2.sai
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bwa aln ref.fa read1.fq > read1.sai; bwa aln ref.fa read2.fq > read2.sai
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bwa samse ref.fa reads.sai reads.fq > aln-pe.sam
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bwa samse ref.fa reads.sai reads.fq > aln-pe.sam
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####Why does a read appear multiple times in the output SAM?
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BWA-SW and BWA-MEM perform local alignments.
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####How to map sequences to GRCh38 with ALT contigs?
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####How to map sequences to GRCh38 with ALT contigs?
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BWA-backtrack and BWA-MEM partially support mapping to a reference containing
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BWA-backtrack and BWA-MEM partially support mapping to a reference containing
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2
bwa.1
2
bwa.1
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@ -191,7 +191,7 @@ yields fewer seeds, which leads to faster alignment speed but lower accuracy. [1
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.BI -c \ INT
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.BI -c \ INT
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Discard a MEM if it has more than
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Discard a MEM if it has more than
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.I INT
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.I INT
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occurence in the genome. This is an insensitive parameter. [10000]
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occurence in the genome. This is an insensitive parameter. [500]
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.TP
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.TP
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.B -P
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.B -P
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In the paired-end mode, perform SW to rescue missing hits only but do not try to find
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In the paired-end mode, perform SW to rescue missing hits only but do not try to find
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@ -156,7 +156,7 @@ int main_mem(int argc, char *argv[])
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fprintf(stderr, "\n");
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fprintf(stderr, "\n");
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fprintf(stderr, " -v INT verbose level: 1=error, 2=warning, 3=message, 4+=debugging [%d]\n", bwa_verbose);
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fprintf(stderr, " -v INT verbose level: 1=error, 2=warning, 3=message, 4+=debugging [%d]\n", bwa_verbose);
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fprintf(stderr, " -T INT minimum score to output [%d]\n", opt->T);
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fprintf(stderr, " -T INT minimum score to output [%d]\n", opt->T);
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fprintf(stderr, " -h INT if #hits < INT, output all in the XA tag [%d]\n", opt->max_hits);
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fprintf(stderr, " -h INT if there are <INT hits with score >80%% of the max score, output all in XA [%d]\n", opt->max_hits);
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fprintf(stderr, " -a output all alignments for SE or unpaired PE\n");
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fprintf(stderr, " -a output all alignments for SE or unpaired PE\n");
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fprintf(stderr, " -C append FASTA/FASTQ comment to SAM output\n");
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fprintf(stderr, " -C append FASTA/FASTQ comment to SAM output\n");
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fprintf(stderr, " -Y use soft clipping for supplementary alignments\n");
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fprintf(stderr, " -Y use soft clipping for supplementary alignments\n");
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