在avx2中加入了一些特殊判断，保证了和串行的sw结果保持基本一致
This commit is contained in:
zzh
parent
5cd4c10858
commit
8f3cb79d6f
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@ -21,7 +21,7 @@
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"~/fastq/diff_r1.fq",
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"~/fastq/diff_r2.fq",
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"-o",
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"/dev/null"
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"out.sam"
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],
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"cwd": "${workspaceFolder}", // 当前工作路径：当前文件所在的工作空间
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},
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2
debug.sh
2
debug.sh
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@ -12,6 +12,8 @@ thread=1
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#n_r2=~/fastq/tiny_n_r2.fq
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n_r1=~/fastq/diff_r1.fq
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n_r2=~/fastq/diff_r2.fq
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#n_r1=~/fastq/diff_all_r1.fq
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#n_r2=~/fastq/diff_all_r2.fq
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#n_r1=~/fastq/d_r1.fq
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#n_r2=~/fastq/d_r2.fq
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reference=~/reference/human_g1k_v37_decoy.fasta
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10
fastmap.c
10
fastmap.c
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@ -64,7 +64,7 @@ int64_t time_ksw_extend2 = 0,
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int64_t dn = 0, n16 = 0, n17 = 0, n18 = 0, n19 = 0, nall = 0, num_sa = 0;
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int64_t s1n = 0, s2n = 0, s3n = 0, s1l = 0, s2l = 0, s3l = 0;
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int64_t g_num_smem2 = 0;
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FILE *fp1;
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FILE *gfp1, *gfp2, *gfp3;
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#endif
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@ -163,7 +163,9 @@ static void update_a(mem_opt_t *opt, const mem_opt_t *opt0)
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int main_mem(int argc, char *argv[])
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{
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#ifdef SHOW_PERF
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fp1 = fopen("./test_out.txt", "w");
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gfp1 = fopen("./fp1.txt", "w");
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gfp2 = fopen("./fp2.txt", "w");
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gfp3 = fopen("./fp3.txt", "w");
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#endif
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mem_opt_t *opt, opt0;
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int fd, fd2, i, c, ignore_alt = 0, no_mt_io = 0;
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@ -443,7 +445,9 @@ int main_mem(int argc, char *argv[])
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fprintf(stderr, "time_ksw_extend2: %f s\n", time_ksw_extend2 / 1000.0 / opt->n_threads);
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fprintf(stderr, "\n");
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fclose(fp1);
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fclose(gfp1);
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fclose(gfp2);
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fclose(gfp3);
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#endif
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return 0;
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@ -6,6 +6,11 @@
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#include <immintrin.h>
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#include <emmintrin.h>
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extern FILE *gfp1, *gfp2, *gfp3;
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//define DEBUG_SW_EXTEND 1
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#define NO_VAL -1
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#ifdef __GNUC__
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#define LIKELY(x) __builtin_expect((x),1)
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#define UNLIKELY(x) __builtin_expect((x),0)
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@ -25,9 +30,8 @@ typedef struct { size_t m; uint8_t *addr; } buf_t;
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extern int ksw_extend2_avx2_u8(int qlen, const uint8_t *query, int tlen, const uint8_t *target, int is_left, int m, const int8_t *mat, int o_del, int e_del,
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int o_ins, int e_ins, int a, int b, int w, int end_bonus, int zdrop, int h0, int *_qle, int *_tle, int *_gtle, int *_gscore, int *_max_off, buf_t *buf);
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int ksw_extend2_origin(int qlen, const uint8_t *query, int tlen, const uint8_t *target, int is_left, int m, const int8_t *mat, int o_del, int e_del,
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int o_ins, int e_ins, int w, int end_bonus, int zdrop, int h0, int *_qle, int *_tle, int *_gtle, int *_gscore, int *_max_off);
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int ksw_extend2_origin(int qlen, const uint8_t *query, int tlen, const uint8_t *target, int is_left, int m, const int8_t *mat, int o_del, int e_del,
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int o_ins, int e_ins, int w, int end_bonus, int zdrop, int h0, int *_qle, int *_tle, int *_gtle, int *_gscore, int *_max_off);
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static const uint16_t h_vec_int_mask[SIMD_WIDTH][SIMD_WIDTH] = {
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{0xffff, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0, 0},
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@ -154,6 +158,7 @@ static const uint16_t h_vec_int_mask[SIMD_WIDTH][SIMD_WIDTH] = {
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int pos = SIMD_WIDTH - 1 - (( __builtin_clz(mask)) >> 1); \
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mj = j - 1 + pos; \
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mi = i - 1 - pos; \
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/*if (m >= max) fprintf(stderr, "%d %d %d %d %d %d %d\n", iend, beg, mi, mj, mask, pos, m);*/ \
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} \
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} \
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}
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@ -192,16 +197,15 @@ int ksw_extend2_avx2(int qlen, // query length 待匹配段碱基的query长度
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buf_t *buf) // 之前已经开辟过的缓存
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{
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//return ksw_extend2_origin(qlen, query, tlen, target, is_left, m, mat, o_del, e_del, o_ins, e_ins, w, end_bonus, zdrop, h0, _qle, _tle, _gtle, _gscore, _max_off);
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// fprintf(stderr, "qlen: %d, tlen: %d\n", qlen, tlen);
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if (qlen * a + h0 < 255) return ksw_extend2_avx2_u8(qlen, query, tlen, target, is_left, m, mat, o_del, e_del, o_ins, e_ins, a, b, w, end_bonus, zdrop, h0, _qle, _tle, _gtle, _gscore, _max_off, buf);
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//if (qlen * a + h0 < 255)
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//return ksw_extend2_avx2_u8(qlen, query, tlen, target, is_left, m, mat, o_del, e_del, o_ins, e_ins, a, b, w, end_bonus, zdrop, h0, _qle, _tle, _gtle, _gscore, _max_off, buf);
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int16_t *mA,*hA, *eA, *fA, *mA1, *mA2, *hA0, *hA1, *eA1, *fA1, *hA2, *eA2, *fA2; // hA0保存上上个col的H，其他的保存上个H E F M
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int16_t *seq, *ref;
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uint8_t *mem;
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int16_t *qtmem, *vmem;
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int seq_size = qlen + SIMD_WIDTH, ref_size = tlen + SIMD_WIDTH;
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int i, iStart, D, j, k, beg, end, max, max_i, max_j, max_ins, max_del, max_ie, gscore, max_off;
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int i, ibeg, D, j, k, beg, end, max, max_i, max_j, max_ins, max_del, max_ie, gscore, max_off;
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int Dloop = tlen + qlen; // 循环跳出条件
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int span, beg1, end1; // 边界条件计算
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int col_size = qlen + 2 + SIMD_WIDTH;
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@ -269,6 +273,34 @@ int ksw_extend2_avx2(int qlen, // query length 待匹配段碱基的query长度
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int m_last=0;
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int iend;
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int m3 = 0, m2 = 0, m1 = 0;
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int midx = 1, icheck = 0, checkspecial = 1;
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int print_flag = 0; //(qlen == 64 && tlen == 123);
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#ifdef DEBUG_SW_EXTEND
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int dii, djj;
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int16_t ins[tlen + 1][qlen + 2];
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int16_t del[tlen + 1][qlen + 2];
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int16_t score[tlen + 1][qlen + 2];
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for (dii = 0; dii <= tlen; ++dii)
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{
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for (djj = 0; djj <= qlen; ++djj)
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{
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ins[dii][djj] = del[dii][djj] = score[dii][djj] = NO_VAL;
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}
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}
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for (dii = 1; dii <= tlen; ++dii)
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{
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del[dii][0] = MAX(0, h0 - o_del - e_del * dii);
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score[dii][0] = del[dii][0];
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}
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for (djj = 1; djj <= qlen; ++djj)
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{
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ins[0][djj] = MAX(0, h0 - o_ins - e_ins * djj);
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score[0][djj] = ins[0][djj];
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}
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ins[0][0] = del[0][0] = score[0][0] = h0;
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#endif
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for (D = 1; LIKELY(D < Dloop); ++D) {
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// 边界条件一定要注意！ tlen 大于，等于，小于 qlen时的情况
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@ -287,18 +319,40 @@ int ksw_extend2_avx2(int qlen, // query length 待匹配段碱基的query长度
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iend = D - (beg - 1); // ref开始计算的位置，倒序
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span = end - beg;
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iStart = iend - span - 1; // 0开始的ref索引位置
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ibeg = iend - span - 1; // 0开始的ref索引位置
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// 每一轮需要记录的数据
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int m = 0, mj = -1, mi = -1;
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max_vec = zero_vec;
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if (print_flag)
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{
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//fprintf(stderr, "D: %d, iend: %d, jbeg: %d\n", D, iend, beg);
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}
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// 要处理边界
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// 左边界 处理f (insert)
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if (iStart == 0) { hA1[end] = MAX(0, h0 - (o_ins + e_ins * end)); }
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if (ibeg == 0) { hA1[end] = MAX(0, h0 - (o_ins + e_ins * end)); }
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// 上边界
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if (beg == 1) { hA1[0] = MAX(0, h0 - (o_del + e_del * iend)); }
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else { hA1[beg-1] = 0; eA1[beg-1] = 0; }
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else {
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if (D & 1) {
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hA1[beg - 1] = 0;
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//eA1[beg - 1] = 0;
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//fA1[beg - 1] = 0;
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hA2[beg - 1] = 0;
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//eA2[beg - 1] = 0;
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//fA2[beg - 1] = 0;
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//mA2[beg - 1] = 0;
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//hA0[beg - 1] = 0;
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}
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else
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{
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//hA0[beg - 1] = 0;
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//hA1[beg - 1] = 0;
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//eA1[beg - 1] = 0;
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//fA1[beg - 1] = 0;
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//mA1[beg - 1] = 0;
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}
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}
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for (j=beg, i=iend; j<=end+1-SIMD_WIDTH; j+=SIMD_WIDTH, i-=SIMD_WIDTH) {
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// 取数据
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@ -326,17 +380,64 @@ int ksw_extend2_avx2(int qlen, // query length 待匹配段碱基的query长度
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SIMD_FIND_MAX;
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if (hA1[0] < 4 && checkspecial) {
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if (hA1[0] == 3) {
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icheck = iend + 1;
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m3 = MAX(m3, hA2[midx]);
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midx += 1;
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} else if (midx == 2) {
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m3 = MAX(m3, hA2[midx]);
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m2 = MAX(m2, hA2[midx - 1]);
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midx += 1;
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} else {
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m3 = MAX(m3, hA2[midx]);
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m2 = MAX(m2, hA2[midx - 1]);
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m1 = MAX(m1, hA2[midx - 2]);
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midx += 1;
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}
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if (ibeg > icheck) {
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if (!m1 || !m2 || !m3)
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break;
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else
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checkspecial = 0;
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}
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if (print_flag) {
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//fprintf(stderr, "jbeg: %d, ibeg: %d, iend: %d, icheck: %d, score: %d %d %d, j: %d\n", beg, ibeg, iend, icheck, hA2[midx + 1], hA2[midx + 2], hA2[midx + 3], midx);
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if (midx > 2) fprintf(stderr, "%d, %d, %d\n", hA2[midx-1], hA2[midx-2], hA2[midx-3]);
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fprintf(stderr, "jbeg: %d, ibeg: %d, iend: %d, icheck: %d, hA1: %d, score: %d %d %d, j: %d\n", beg, ibeg, iend, icheck, hA1[0], m1, m2, m3, midx);
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}
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}
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#ifdef DEBUG_SW_EXTEND
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for (djj = beg; djj <= end; ++djj)
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{
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dii = D - djj + 1;
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ins[dii][djj] = fA2[djj];
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del[dii][djj] = eA2[djj];
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score[dii][djj] = hA2[djj];
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}
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if (print_flag)
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{
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//fprintf(stderr, "score: %d %d %d\n", hA2[beg], hA2[beg+1], hA2[beg+2]);
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}
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#endif
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// 注意最后跳出循环j的值
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j = end + 1;
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if (j == qlen + 1) {
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max_ie = gscore > hA2[qlen] ? max_ie : iStart;
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max_ie = gscore > hA2[qlen] ? max_ie : ibeg;
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gscore = gscore > hA2[qlen] ? gscore : hA2[qlen];
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}
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if (m == 0 && m_last==0) break; // 一定要注意，斜对角遍历和按列遍历的不同点
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//if (m == 0 && m_last < 2) break;
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if (m > max) {
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max = m, max_i = mi, max_j = mj;
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max_off = max_off > abs(mj - mi)? max_off : abs(mj - mi);
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max_off = max_off > abs(mj - mi) ? max_off : abs(mj - mi);
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} else if (m == max && max_i >= mi && mj > max_j) {
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max_i = mi, max_j = mj;
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max_off = max_off > abs(mj - mi) ? max_off : abs(mj - mi);
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}
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else if (zdrop > 0) {
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if (mi - max_i > mj - max_j) {
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@ -357,7 +458,49 @@ int ksw_extend2_avx2(int qlen, // query length 待匹配段碱基的query长度
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SWAP_DATA_POINTER;
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}
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// free(mem);
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#ifdef DEBUG_SW_EXTEND
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fprintf(gfp1, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
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fprintf(gfp2, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
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fprintf(gfp3, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
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#endif
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#ifdef DEBUG_SW_EXTEND
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fprintf(gfp1, "%-4d", -1);
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fprintf(gfp2, "%-4d", -1);
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fprintf(gfp3, "%-4d", -1);
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fprintf(gfp1, "%-4d", -1);
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fprintf(gfp2, "%-4d", -1);
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fprintf(gfp3, "%-4d", -1);
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for (djj = 0; djj < qlen; ++djj) {
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fprintf(gfp1, "%-4c", "ACGTN"[query[djj]]);
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fprintf(gfp2, "%-4c", "ACGTN"[query[djj]]);
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fprintf(gfp3, "%-4c", "ACGTN"[query[djj]]);
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}
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fprintf(gfp1, "\n");
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fprintf(gfp2, "\n");
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fprintf(gfp3, "\n");
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for (dii = 0; dii <= tlen; ++dii)
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{
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if (dii > 0) {
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fprintf(gfp1, "%-4c", "ACGTN"[target[dii - 1]]);
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fprintf(gfp2, "%-4c", "ACGTN"[target[dii - 1]]);
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fprintf(gfp3, "%-4c", "ACGTN"[target[dii - 1]]);
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} else {
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fprintf(gfp1, "%-4d", -1);
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fprintf(gfp2, "%-4d", -1);
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fprintf(gfp3, "%-4d", -1);
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}
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for (djj = 0; djj <= qlen; ++djj)
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{
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fprintf(gfp1, "%-4d", score[dii][djj]);
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fprintf(gfp2, "%-4d", ins[dii][djj]);
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fprintf(gfp3, "%-4d", del[dii][djj]);
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}
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fprintf(gfp1, "\n");
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fprintf(gfp2, "\n");
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fprintf(gfp3, "\n");
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}
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#endif
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// free(mem);
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if (_qle) *_qle = max_j + 1;
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if (_tle) *_tle = max_i + 1;
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if (_gtle) *_gtle = max_ie + 1;
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@ -427,12 +570,39 @@ int ksw_extend2_origin(int qlen, // query length 待匹配段碱基的query长
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max_del = (int)((double)(qlen * max + end_bonus - o_del) / e_del + 1.);
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max_del = max_del > 1? max_del : 1;
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w = w < max_del? w : max_del; // TODO: is this necessary?
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// printf("%d\n", w);
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//fprintf(stderr, "%d\n", w);
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// DP loop
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max = h0, max_i = max_j = -1; max_ie = -1, gscore = -1;
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max_off = 0;
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beg = 0, end = qlen;
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int print_flag = 0; //(qlen == 116 && tlen == 241);
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//fprintf(stderr, "%d %d %d\n", print_flag, qlen, tlen);
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#ifdef DEBUG_SW_EXTEND
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int dii, djj;
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int16_t ins[tlen + 1][qlen + 2];
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int16_t del[tlen + 1][qlen + 2];
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int16_t score[tlen + 1][qlen + 2];
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for (dii = 0; dii <= tlen; ++dii)
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{
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for (djj = 0; djj <= qlen; ++djj)
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{
|
||||
ins[dii][djj] = del[dii][djj] = score[dii][djj] = NO_VAL;
|
||||
}
|
||||
}
|
||||
for (dii = 1; dii <= tlen; ++dii)
|
||||
{
|
||||
del[dii][0] = MAX(0, h0 - o_del - e_del * dii);
|
||||
score[dii][0] = del[dii][0];
|
||||
}
|
||||
for (djj = 1; djj <= qlen; ++djj)
|
||||
{
|
||||
ins[0][djj] = MAX(0, h0 - o_ins - e_ins * djj);
|
||||
score[0][djj] = ins[0][djj];
|
||||
}
|
||||
ins[0][0] = del[0][0] = score[0][0] = h0;
|
||||
#endif
|
||||
|
||||
for (i = 0; LIKELY(i < tlen); ++i) {
|
||||
int t, f = 0, h1, m = 0, mj = -1;
|
||||
int8_t *q = &qp[ref[i] * qlen];
|
||||
|
|
@ -445,7 +615,11 @@ int ksw_extend2_origin(int qlen, // query length 待匹配段碱基的query长
|
|||
h1 = h0 - (o_del + e_del * (i + 1));
|
||||
if (h1 < 0) h1 = 0;
|
||||
} else h1 = 0;
|
||||
//m = h1;
|
||||
for (j = beg; LIKELY(j < end); ++j) {
|
||||
#ifdef DEBUG_SW_EXTEND
|
||||
ins[i+1][j+1] = f;
|
||||
#endif
|
||||
// At the beginning of the loop: eh[j] = { H(i-1,j-1), E(i,j) }, f = F(i,j) and h1 = H(i,j-1)
|
||||
// Similar to SSE2-SW, cells are computed in the following order:
|
||||
// H(i,j) = max{H(i-1,j-1)+S(i,j), E(i,j), F(i,j)}
|
||||
|
|
@ -454,16 +628,22 @@ int ksw_extend2_origin(int qlen, // query length 待匹配段碱基的query长
|
|||
eh_t *p = &eh[j];
|
||||
int h, M = p->h, e = p->e; // get H(i-1,j-1) and E(i-1,j)
|
||||
p->h = h1; // set H(i,j-1) for the next row
|
||||
M = M? M + q[j] : 0;// separating H and M to disallow a cigar like "100M3I3D20M"
|
||||
M = M? M + q[j] : 0;// separating H and M to disallow a cigar like "100M3I3D20M",保证分值不小于0，sw和nw的区别
|
||||
h = M > e? M : e; // e and f are guaranteed to be non-negative, so h>=0 even if M<0
|
||||
h = h > f? h : f;
|
||||
h1 = h; // save H(i,j) to h1 for the next column
|
||||
#ifdef DEBUG_SW_EXTEND
|
||||
score[i+1][j+1] = h;
|
||||
#endif
|
||||
mj = m > h? mj : j; // record the position where max score is achieved
|
||||
m = m > h? m : h; // m is stored at eh[mj+1]
|
||||
t = M - oe_del;
|
||||
t = t > 0? t : 0;
|
||||
e -= e_del;
|
||||
e = e > t? e : t; // computed E(i+1,j)
|
||||
#ifdef DEBUG_SW_EXTEND
|
||||
del[i+1][j+1] = e;
|
||||
#endif
|
||||
p->e = e; // save E(i+1,j) for the next row
|
||||
t = M - oe_ins;
|
||||
t = t > 0? t : 0;
|
||||
|
|
@ -479,6 +659,7 @@ int ksw_extend2_origin(int qlen, // query length 待匹配段碱基的query长
|
|||
if (m > max) {
|
||||
max = m, max_i = i, max_j = mj;
|
||||
max_off = max_off > abs(mj - i)? max_off : abs(mj - i);
|
||||
//fprintf(stderr, "%d %d %d %d\n", i, mj, max_off, m);
|
||||
} else if (zdrop > 0) {
|
||||
if (i - max_i > mj - max_j) {
|
||||
if (max - m - ((i - max_i) - (mj - max_j)) * e_del > zdrop) break;
|
||||
|
|
@ -487,12 +668,61 @@ int ksw_extend2_origin(int qlen, // query length 待匹配段碱基的query长
|
|||
}
|
||||
}
|
||||
// update beg and end for the next round
|
||||
for (j = beg; LIKELY(j < end) && eh[j].h == 0 && eh[j].e == 0; ++j);
|
||||
for (j = beg; LIKELY(j < end) && eh[j].h == 0 && eh[j].e == 0; ++j); // 这里为什么不考虑f（insert score）
|
||||
beg = j;
|
||||
for (j = end; LIKELY(j >= beg) && eh[j].h == 0 && eh[j].e == 0; --j);
|
||||
end = j + 2 < qlen? j + 2 : qlen;
|
||||
//beg = 0; end = qlen; // uncomment this line for debugging
|
||||
if (print_flag) {
|
||||
fprintf(stderr, "beg: %d; end: %d\n", beg, end);
|
||||
}
|
||||
}
|
||||
#ifdef DEBUG_SW_EXTEND
|
||||
fprintf(gfp1, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
|
||||
fprintf(gfp2, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
|
||||
fprintf(gfp3, "qlen: %d, tlen: %d, h0: %d, w: %d, mi: %d, mj: %d, mie: %d, max_off: %d, score: %d, max: %d\n", qlen, tlen, h0, w, max_i + 1, max_j + 1, max_ie + 1, max_off, gscore, max);
|
||||
#endif
|
||||
#ifdef DEBUG_SW_EXTEND
|
||||
fprintf(gfp1, "%-4d", -1);
|
||||
fprintf(gfp2, "%-4d", -1);
|
||||
fprintf(gfp3, "%-4d", -1);
|
||||
fprintf(gfp1, "%-4d", -1);
|
||||
fprintf(gfp2, "%-4d", -1);
|
||||
fprintf(gfp3, "%-4d", -1);
|
||||
for (djj = 0; djj < qlen; ++djj)
|
||||
{
|
||||
fprintf(gfp1, "%-4c", "ACGTN"[query[djj]]);
|
||||
fprintf(gfp2, "%-4c", "ACGTN"[query[djj]]);
|
||||
fprintf(gfp3, "%-4c", "ACGTN"[query[djj]]);
|
||||
}
|
||||
fprintf(gfp1, "\n");
|
||||
fprintf(gfp2, "\n");
|
||||
fprintf(gfp3, "\n");
|
||||
for (dii = 0; dii <= tlen; ++dii)
|
||||
{
|
||||
if (dii > 0)
|
||||
{
|
||||
fprintf(gfp1, "%-4c", "ACGTN"[target[dii - 1]]);
|
||||
fprintf(gfp2, "%-4c", "ACGTN"[target[dii - 1]]);
|
||||
fprintf(gfp3, "%-4c", "ACGTN"[target[dii - 1]]);
|
||||
}
|
||||
else
|
||||
{
|
||||
fprintf(gfp1, "%-4d", -1);
|
||||
fprintf(gfp2, "%-4d", -1);
|
||||
fprintf(gfp3, "%-4d", -1);
|
||||
}
|
||||
for (djj = 0; djj <= qlen; ++djj)
|
||||
{
|
||||
fprintf(gfp1, "%-4d", score[dii][djj]);
|
||||
fprintf(gfp2, "%-4d", ins[dii][djj]);
|
||||
fprintf(gfp3, "%-4d", del[dii][djj]);
|
||||
}
|
||||
fprintf(gfp1, "\n");
|
||||
fprintf(gfp2, "\n");
|
||||
fprintf(gfp3, "\n");
|
||||
}
|
||||
#endif
|
||||
|
||||
free(eh); free(qp); free(qmem);
|
||||
if (_qle) *_qle = max_j + 1;
|
||||
|
|
|
|||
|
|
@ -213,7 +213,7 @@ int ksw_extend2_avx2_u8(int qlen, // query length 待匹配段碱基的query长
|
|||
uint8_t *seq, *ref;
|
||||
uint8_t *mem, *qtmem, *vmem;
|
||||
int seq_size = qlen + SIMD_WIDTH, ref_size = tlen + SIMD_WIDTH;
|
||||
int i, iStart, D, j, k, beg, end, max, max_i, max_j, max_ins, max_del, max_ie, gscore, max_off;
|
||||
int i, ibeg, D, j, k, beg, end, max, max_i, max_j, max_ins, max_del, max_ie, gscore, max_off;
|
||||
int Dloop = tlen + qlen; // 循环跳出条件
|
||||
int span, beg1, end1; // 边界条件计算
|
||||
int col_size = qlen + 2 + SIMD_WIDTH;
|
||||
|
|
@ -299,7 +299,7 @@ int ksw_extend2_avx2_u8(int qlen, // query length 待匹配段碱基的query长
|
|||
|
||||
iend = D - (beg - 1); // ref开始计算的位置，倒序
|
||||
span = end - beg;
|
||||
iStart = iend - span - 1; // 0开始的ref索引位置
|
||||
ibeg = iend - span - 1; // 0开始的ref索引位置
|
||||
|
||||
// 每一轮需要记录的数据
|
||||
int m = 0, mj = -1, mi = -1;
|
||||
|
|
@ -307,10 +307,13 @@ int ksw_extend2_avx2_u8(int qlen, // query length 待匹配段碱基的query长
|
|||
|
||||
// 要处理边界
|
||||
// 左边界 处理f (insert)
|
||||
if (iStart == 0) { hA1[end] = MAX(0, h0 - (o_ins + e_ins * end)); }
|
||||
if (ibeg == 0) { hA1[end] = MAX(0, h0 - (o_ins + e_ins * end)); }
|
||||
// 上边界
|
||||
if (beg == 1) { hA1[0] = MAX(0, h0 - (o_del + e_del * iend)); }
|
||||
else { hA1[beg-1] = 0; eA1[beg-1] = 0; }
|
||||
else if (D & 1) {
|
||||
hA1[beg - 1] = 0;
|
||||
eA1[beg - 1] = 0;
|
||||
fA1[beg - 1] = 0; }
|
||||
|
||||
for (j=beg, i=iend; j<=end+1-SIMD_WIDTH; j+=SIMD_WIDTH, i-=SIMD_WIDTH) {
|
||||
// 取数据
|
||||
|
|
@ -342,7 +345,7 @@ int ksw_extend2_avx2_u8(int qlen, // query length 待匹配段碱基的query长
|
|||
j = end + 1;
|
||||
|
||||
if (j == qlen + 1) {
|
||||
max_ie = gscore > hA2[qlen] ? max_ie : iStart;
|
||||
max_ie = gscore > hA2[qlen] ? max_ie : ibeg;
|
||||
gscore = gscore > hA2[qlen] ? gscore : hA2[qlen];
|
||||
}
|
||||
if (m == 0 && m_last==0) break; // 一定要注意，斜对角遍历和按列遍历的不同点
|
||||
|
|
@ -350,6 +353,10 @@ int ksw_extend2_avx2_u8(int qlen, // query length 待匹配段碱基的query长
|
|||
max = m, max_i = mi, max_j = mj;
|
||||
max_off = max_off > abs(mj - mi)? max_off : abs(mj - mi);
|
||||
}
|
||||
else if (m == max && max_i >= mi && mj > max_j) {
|
||||
max_i = mi, max_j = mj;
|
||||
max_off = max_off > abs(mj - mi) ? max_off : abs(mj - mi);
|
||||
}
|
||||
else if (zdrop > 0) {
|
||||
if (mi - max_i > mj - max_j) {
|
||||
if (max - m - ((mi - max_i) - (mj - max_j)) * e_del > zdrop) break;
|
||||
|
|
|
|||
108
out.sam
108
out.sam
|
|
@ -1,108 +0,0 @@
|
|||
@SQ SN:1 LN:249250621
|
||||
@SQ SN:2 LN:243199373
|
||||
@SQ SN:3 LN:198022430
|
||||
@SQ SN:4 LN:191154276
|
||||
@SQ SN:5 LN:180915260
|
||||
@SQ SN:6 LN:171115067
|
||||
@SQ SN:7 LN:159138663
|
||||
@SQ SN:8 LN:146364022
|
||||
@SQ SN:9 LN:141213431
|
||||
@SQ SN:10 LN:135534747
|
||||
@SQ SN:11 LN:135006516
|
||||
@SQ SN:12 LN:133851895
|
||||
@SQ SN:13 LN:115169878
|
||||
@SQ SN:14 LN:107349540
|
||||
@SQ SN:15 LN:102531392
|
||||
@SQ SN:16 LN:90354753
|
||||
@SQ SN:17 LN:81195210
|
||||
@SQ SN:18 LN:78077248
|
||||
@SQ SN:19 LN:59128983
|
||||
@SQ SN:20 LN:63025520
|
||||
@SQ SN:21 LN:48129895
|
||||
@SQ SN:22 LN:51304566
|
||||
@SQ SN:X LN:155270560
|
||||
@SQ SN:Y LN:59373566
|
||||
@SQ SN:MT LN:16569
|
||||
@SQ SN:GL000207.1 LN:4262
|
||||
@SQ SN:GL000226.1 LN:15008
|
||||
@SQ SN:GL000229.1 LN:19913
|
||||
@SQ SN:GL000231.1 LN:27386
|
||||
@SQ SN:GL000210.1 LN:27682
|
||||
@SQ SN:GL000239.1 LN:33824
|
||||
@SQ SN:GL000235.1 LN:34474
|
||||
@SQ SN:GL000201.1 LN:36148
|
||||
@SQ SN:GL000247.1 LN:36422
|
||||
@SQ SN:GL000245.1 LN:36651
|
||||
@SQ SN:GL000197.1 LN:37175
|
||||
@SQ SN:GL000203.1 LN:37498
|
||||
@SQ SN:GL000246.1 LN:38154
|
||||
@SQ SN:GL000249.1 LN:38502
|
||||
@SQ SN:GL000196.1 LN:38914
|
||||
@SQ SN:GL000248.1 LN:39786
|
||||
@SQ SN:GL000244.1 LN:39929
|
||||
@SQ SN:GL000238.1 LN:39939
|
||||
@SQ SN:GL000202.1 LN:40103
|
||||
@SQ SN:GL000234.1 LN:40531
|
||||
@SQ SN:GL000232.1 LN:40652
|
||||
@SQ SN:GL000206.1 LN:41001
|
||||
@SQ SN:GL000240.1 LN:41933
|
||||
@SQ SN:GL000236.1 LN:41934
|
||||
@SQ SN:GL000241.1 LN:42152
|
||||
@SQ SN:GL000243.1 LN:43341
|
||||
@SQ SN:GL000242.1 LN:43523
|
||||
@SQ SN:GL000230.1 LN:43691
|
||||
@SQ SN:GL000237.1 LN:45867
|
||||
@SQ SN:GL000233.1 LN:45941
|
||||
@SQ SN:GL000204.1 LN:81310
|
||||
@SQ SN:GL000198.1 LN:90085
|
||||
@SQ SN:GL000208.1 LN:92689
|
||||
@SQ SN:GL000191.1 LN:106433
|
||||
@SQ SN:GL000227.1 LN:128374
|
||||
@SQ SN:GL000228.1 LN:129120
|
||||
@SQ SN:GL000214.1 LN:137718
|
||||
@SQ SN:GL000221.1 LN:155397
|
||||
@SQ SN:GL000209.1 LN:159169
|
||||
@SQ SN:GL000218.1 LN:161147
|
||||
@SQ SN:GL000220.1 LN:161802
|
||||
@SQ SN:GL000213.1 LN:164239
|
||||
@SQ SN:GL000211.1 LN:166566
|
||||
@SQ SN:GL000199.1 LN:169874
|
||||
@SQ SN:GL000217.1 LN:172149
|
||||
@SQ SN:GL000216.1 LN:172294
|
||||
@SQ SN:GL000215.1 LN:172545
|
||||
@SQ SN:GL000205.1 LN:174588
|
||||
@SQ SN:GL000219.1 LN:179198
|
||||
@SQ SN:GL000224.1 LN:179693
|
||||
@SQ SN:GL000223.1 LN:180455
|
||||
@SQ SN:GL000195.1 LN:182896
|
||||
@SQ SN:GL000212.1 LN:186858
|
||||
@SQ SN:GL000222.1 LN:186861
|
||||
@SQ SN:GL000200.1 LN:187035
|
||||
@SQ SN:GL000193.1 LN:189789
|
||||
@SQ SN:GL000194.1 LN:191469
|
||||
@SQ SN:GL000225.1 LN:211173
|
||||
@SQ SN:GL000192.1 LN:547496
|
||||
@SQ SN:NC_007605 LN:171823
|
||||
@SQ SN:hs37d5 LN:35477943
|
||||
@HD VN:1.5 SO:unsorted GO:query
|
||||
@RG ID:normal SM:normal PL:illumina LB:normal PG:bwa
|
||||
@PG ID:bwa PN:bwa VN:0.7.17-r1198-dirty CL:./bwa mem -t 1 -M -R @RG\tID:normal\tSM:normal\tPL:illumina\tLB:normal\tPG:bwa /home/zzh/reference/human_g1k_v37_decoy.fasta /home/zzh/fastq/diff_r1.fq /home/zzh/fastq/diff_r2.fq -o ./out.sam
|
||||
A00289:748:HLCH3DSX3:4:1102:24749:34929 97 16 75642166 60 150M 12 66451372 0 AAAGCCATTGGCAGTAACATCCAAAGGCTGCTCAGGAACTGCACTCCAGCTGATGGCTATGAAAAGATAAGATTCTAGGGTTAAAACTGCTCAATTTCTTGTGAAAGTCAGAAGTATTTTAATAAAAATATTTCTCTGACTCTACAGACC FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:150 MC:Z:17S91M42S AS:i:150 XS:i:0 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1102:24749:34929 145 12 66451372 0 17S91M42S 16 75642166 0 TTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAATAATTCTTTGTGAAACCCCT F,F,FFF,:FFF,,F,F,,F,F:,FFF:F:FFF:FFFFFFF:F,F,F,FFFFFF::,,FFFFFFF,:F,:,F,::,,F:FF:,FF:F::F::FFFFFFFFFF,F:F,FFFF,FFFFF,FF,F,,F,F,,,,,,:,,,,,,,,,,,,,,,F NM:i:0 MD:Z:91 MC:Z:150M AS:i:91 XS:i:90 RG:Z:normal SA:Z:12,49106363,+,117S33M,0,0; XA:Z:12,-66451373,35S90M25S,0;12,-66451373,28S90M32S,0;6,-160521757,23S83M44S,0;15,-77910871,23S79M48S,0;
|
||||
A00289:748:HLCH3DSX3:4:1102:24749:34929 385 12 49106363 0 117H33M 16 75642166 0 AAAAAAAAAAAAAAATAAAAAAAAAAAAAAAAA FFF:F:FFF,:F,F,,F,F,,FFF:,FFF,F,F NM:i:0 MD:Z:33 MC:Z:150M AS:i:33 XS:i:32 RG:Z:normal SA:Z:12,66451372,-,17S91M42S,0,0; XA:Z:4,-19079503,32M118S,0;15,-55218248,32M118S,0;X,-149551159,32M118S,0;
|
||||
A00289:748:HLCH3DSX3:4:1104:6234:8656 113 12 66451373 8 12S91M47S 22 18873826 0 TTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGTTTTTTTTTTTTTTGGGGGGGGGGGGGGGTGAGGTGGTCTGTG ,,:FFF:,:,::,,,,,,FF,FFF,,FF,F,FFF:,,:FFFFFF:FFF,:,:FF:F:::::FFF::,::F:FFFFFF:,FFFF,,,:FFFFFFFF:,,::,,,,,:,,FFF:,,,FF:F,:,FFFF:F::,,FF:,:FFFFFF:FFFFFF NM:i:1 MD:Z:13T77 MC:Z:150M AS:i:86 XS:i:81 RG:Z:normal SA:Z:3,121668661,-,89S32M29S,0,0; XA:Z:12,-66451372,25S91M34S,2;6,-160521757,19S84M47S,1;15,-77910874,26S77M47S,0;7,-107410599,25M1I3M15D74M47S,16;
|
||||
A00289:748:HLCH3DSX3:4:1104:6234:8656 369 3 121668661 0 89H32M29H 22 18873826 0 TTTTTTTTTTTTTGTTTGTTTTTTTTTTTTTT FFFFFF:,,::,,,,,:,,FFF:,,,FF:F,: NM:i:0 MD:Z:32 MC:Z:150M AS:i:32 XS:i:32 RG:Z:normal SA:Z:12,66451373,-,12S91M47S,8,1;
|
||||
A00289:748:HLCH3DSX3:4:1104:6234:8656 177 22 18873826 22 150M 12 66451373 0 CTATACCCCACATCCTCATTCCCACCAGATGTCTTGGATCATGGAGGCTCTCCAGACAAAAGCCAGCAGTTAAGCTCCAGATTTCCTATAGAATCCTTTTCTAACAACCAGTGAGTGATTCCAGAATACGTACCATTGAATGTGCTCCCT FFF:FFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:150 MC:Z:12S91M47S AS:i:150 XS:i:140 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1104:1741:18129 97 11 113220686 60 150M 12 66451373 0 TATTTCACCCGGATGCTGACTCCTTTTTTGGTTTCCTTGCAAGGTTACCCTGTTGGGATGTTGTTAGTTGGAGGACAGCTACCTCTGAGGTTATTATGGTTTTTTGCAGATTATTGGAAGCGCTGGTGTCATTTCTTGATTTCTCGGATA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFFFF:FFFFFFF:FFFF NM:i:0 MD:Z:150 MC:Z:90M60S AS:i:150 XS:i:19 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1104:1741:18129 145 12 66451373 7 90M60S 11 113220686 0 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTCCCAC FF:FFFF,F,,FFFFFFFF:FFFF:,F,,:F:F::::,F::::FFF::F::,,:FFF,:::FFFFFFFFFFF:FFFFFFFF,FFFF:FFF,:,:,,::FFFFFFFFFFF,FF:FFF:F:F:F:,F,,::F,,F,,FFFFF:F:,,,,,,, NM:i:1 MD:Z:81T8 MC:Z:150M AS:i:85 XS:i:81 RG:Z:normal SA:Z:8,112168232,-,79S59M12S,0,1; XA:Z:6,-160521757,81M69S,0;4,-82515382,13S69M68S,0;
|
||||
A00289:748:HLCH3DSX3:4:1104:1741:18129 401 8 112168232 0 79H59M12H 11 113220686 0 TTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTT FF,FFFF:FFF,:,:,,::FFFFFFFFFFF,FF:FFF:F:F:F:,F,,::F,,F,,FFF NM:i:1 MD:Z:46T12 MC:Z:150M AS:i:54 XS:i:53 RG:Z:normal SA:Z:12,66451373,-,90M60S,7,1;
|
||||
A00289:748:HLCH3DSX3:4:1105:27281:29700 113 X 117117442 60 150M 12 66451372 0 CCCTGAAGTTGATTTTTTGAATATATCCTTGAAATCGGCTTGTTTTAATGACAATTGCCTCCGTCTCCTGAAAGTGGAGTGCAAAAAGAAACCTGGTTAATTGTAGTTTTATTATATTTAGTTCAAATTTTTCAAAGCATACCCTGACAG FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:150 MC:Z:12S89M49S AS:i:150 XS:i:0 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1105:27281:29700 177 12 66451372 0 12S89M49S X 117117442 0 TTTTTTAAAAATATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTATTTATTTTTTTATTTTTTTATTATTTTTTTTTTTTTTTTTTTTTTTTTGGTGCCGCCCCC ,,,,:,,F:F,,,,:FF,F:FF,::F:F:F,,F::FFF,FFFFFFFFF,FFFFFF::FFF:F:FFFFFFF,FFFFFFF::FFFF:,:FF:,,F,:,FFFFF,:,,:F,,,,:,FFFFF:F,,FFF:FFFFF:,F,F,,,:,,,,,:FFFF NM:i:3 MD:Z:73T3T3T7 MC:Z:150M AS:i:74 XS:i:72 RG:Z:normal SA:Z:15,85942734,+,20S48M82S,0,1;
|
||||
A00289:748:HLCH3DSX3:4:1105:27281:29700 417 15 85942734 0 20H48M82H X 117117442 0 AAAAAAAAAAAAAAAAATAATAAAAAAATAAAAAAATAAATAAATAAA FFFF:FFF,,F:FFFFF,:,,,,F:,,:,FFFFF,:,F,,:FF:,:FF NM:i:1 MD:Z:17A30 MC:Z:150M AS:i:43 XS:i:36 RG:Z:normal SA:Z:12,66451372,-,12S89M49S,0,3; XA:Z:11,-118049564,69S35M7D34M12S,11;2,+204499190,12S31M1D21M86S,3;
|
||||
A00289:748:HLCH3DSX3:4:1106:2880:19288 97 4 169630630 0 29M1I4M1I68M9D22M25S 12 66451373 0 CAGCCTGGGTGACAGAGTGAGACTCCAGCTAAGGCAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAAGGAATGAAGGAAGGAAGGAGGGAGGGAGGGAGGGAGGGAGAGATCGGAAGAGCACACGTCTGAAC FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFF:FFF:FFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFF NM:i:14 MD:Z:18C8T59G13^AAGGAAGGA22 MC:Z:36S91M23S AS:i:73 XS:i:72 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1106:2880:19288 145 12 66451373 9 36S91M23S 4 169630630 0 TATATACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGGGTGGGTGGGTGGGTTGGCAG :,,,,,,,:FF:FFFF:,:FF:,F,F,FFF,,,FFF:FFFFFFFFF,:,,F:F,FFFF,:FFFFFFFFFFFFFFFFFFFF:FF:FFFF:FFFFFFF:FFF:FFFFFFF:FF,FFF:,:::,:F::,,:,,:,:,,,,,:,::,,,:F,FF NM:i:1 MD:Z:10T80 MC:Z:29M1I4M1I68M9D22M25S AS:i:86 XS:i:80 RG:Z:normal SA:Z:15,55218228,-,9S52M89S,0,0; XA:Z:15,-77910871,47S80M23S,0;6,-160521761,47S80M23S,0;6,-160521757,47S79M24S,0;7,-107410599,25S28M15D74M23S,16;
|
||||
A00289:748:HLCH3DSX3:4:1106:2880:19288 401 15 55218228 0 9H52M89H 4 169630630 0 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTT FF:FFFF:,:FF:,F,F,FFF,,,FFF:FFFFFFFFF,:,,F:F,FFFF,:F NM:i:0 MD:Z:52 MC:Z:29M1I4M1I68M9D22M25H AS:i:52 XS:i:50 RG:Z:normal SA:Z:12,66451373,-,36S91M23S,9,1;
|
||||
A00289:748:HLCH3DSX3:4:1107:2365:20462 97 22 18737670 0 150M 12 66451372 0 GAGGACACCATACAAGCCGTCGCTAACAAGGACACTGTACACAACATCGCTAATGACGGCACCGTACAAGACATCACCAATGAGGGCGCTTTATACGACATTGCTAATGATACCGACAAGGCACGCTAACGTGGACGCTGTACACGACAT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:150 MC:Z:21S90M39S AS:i:150 XS:i:150 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1107:2365:20462 145 12 66451372 13 21S90M39S 22 18737670 0 TTTTATATATTTATATTTATTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTGTTTTTTGTGGTGGGGGGGGGGTTTGGCATAGCAT F:F,,F,F,F:FFF,,,:,F:,:,,:,,,,:,F:,,FFFFFFFFFFFFF,F:FFFFFFFFF:FFFF,,FFFFF:FFF::FFFFF,F:F,,F:F,FFFFFFFF,:F:F::::,,:F,F,,F,:,,,,,::,,,,:,,:,,,,,,F,F::FF NM:i:0 MD:Z:90 MC:Z:150M AS:i:90 XS:i:84 RG:Z:normal XA:Z:6,-160521757,28S84M38S,0;15,-77910871,32S80M38S,0;7,-107410642,38S74M38S,0;
|
||||
A00289:748:HLCH3DSX3:4:1107:26377:28385 113 GL000220.1 122227 60 150M 6 160521757 0 TGCTCTGTTGCTCACGCTGGTCTCAAACTCCTGGCCTTGACGCTTCTCCCGTCACATCCGCCGTCTGGTTGTTGAAATGAGCATCTCTCGTAAAATGGAAAAGATGAAAGAAATAAACACGAAGACGGAAAGCACGGTGTGAACGTTTCT FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:150 MC:Z:85M65S AS:i:150 XS:i:113 RG:Z:normal
|
||||
A00289:748:HLCH3DSX3:4:1107:26377:28385 177 6 160521757 0 85M65S GL000220.1 122227 0 TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTGTTTTTTTTTTGTTGGGGGTTGGGTGTTGGGGGGCGGGGGGGGGGGCGCCCCCCCCCAAGGAGA F:FF,F,,F,F,FFFFFF,:FF,:FF:F,:FF,FFFF:FFF:FF,FFFFFFFFFFFFFFFFFFFFFFFF:FF,F,F:F,:FF,,,FFFF,F,:,,,,,:::,,:,,,,::,,,,,,,,,F,,FF,:F:,,F,,,,,FF:FF:F,FFFF:: NM:i:0 MD:Z:85 MC:Z:150M AS:i:85 XS:i:84 RG:Z:normal XA:Z:12,-66451380,84M66S,0;12,-66451373,83M67S,0;15,-77910871,4S80M66S,0;
|
||||
15
run.sh
15
run.sh
|
|
@ -1,15 +1,15 @@
|
|||
thread=1
|
||||
thread=32
|
||||
#n_r1=~/data/fastq/ZY2105177532213000/n_r1.fq
|
||||
#n_r2=~/data/fastq/ZY2105177532213000/n_r2.fq
|
||||
#n_r1=~/data/fastq/ZY2105177532213000/sn_r1.fq
|
||||
#n_r2=~/data/fastq/ZY2105177532213000/sn_r2.fq
|
||||
#reference=~/data/reference/human_g1k_v37_decoy.fasta
|
||||
#n_r1=~/fastq/sn_r1.fq
|
||||
#n_r2=~/fastq/sn_r2.fq
|
||||
n_r1=~/fastq/sn_r1.fq
|
||||
n_r2=~/fastq/sn_r2.fq
|
||||
#n_r1=~/fastq/ssn_r1.fq
|
||||
#n_r2=~/fastq/ssn_r2.fq
|
||||
n_r1=~/fastq/ERR194147_1_120w.fastq
|
||||
n_r2=~/fastq/ERR194147_2_120w.fastq
|
||||
#n_r1=~/fastq/ERR194147_1_120w.fastq
|
||||
#n_r2=~/fastq/ERR194147_2_120w.fastq
|
||||
#n_r1=~/fastq/tiny_n_r1.fq
|
||||
#n_r2=~/fastq/tiny_n_r2.fq
|
||||
#n_r1=~/fastq/diff_r1.fq
|
||||
|
|
@ -18,9 +18,10 @@ n_r2=~/fastq/ERR194147_2_120w.fastq
|
|||
#n_r2=~/fastq/d_r2.fq
|
||||
reference=~/reference/human_g1k_v37_decoy.fasta
|
||||
#out=./all.sam
|
||||
#out=./ssn.sam
|
||||
out=./sn.sam
|
||||
#out=./out.sam
|
||||
out=/dev/null
|
||||
#out=/dev/null
|
||||
#out=na12878.sam
|
||||
#time ./bwa mem -t 12 -M -R @RG\\tID:normal\\tSM:normal\\tPL:illumina\\tLB:normal\\tPG:bwa \
|
||||
# /home/zzh/data/reference/human_g1k_v37_decoy.fasta \
|
||||
# /home/zzh/data/fastq/nm1.fq \
|
||||
|
|
|
|||
Loading…
Reference in New Issue