Released bwa-0.7.4-r385
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bwa.1
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bwa.1
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.TH bwa 1 "15 March 2013" "bwa-0.7.4" "Bioinformatics tools"
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.TH bwa 1 "23 April 2013" "bwa-0.7.4" "Bioinformatics tools"
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.SH NAME
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.PP
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bwa - Burrows-Wheeler Alignment Tool
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@ -44,7 +44,12 @@ for the BWA-MEM algorithm.
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.SH COMMANDS AND OPTIONS
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.TP
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.B index
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bwa index [-p prefix] [-a algoType] <in.db.fasta>
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.B bwa index
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.RB [ -p
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.IR prefix ]
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.RB [ -a
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.IR algoType ]
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.I db.fa
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Index database sequences in the FASTA format.
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@ -55,20 +60,16 @@ Index database sequences in the FASTA format.
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Prefix of the output database [same as db filename]
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.TP
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.BI -a \ STR
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Algorithm for constructing BWT index. Available options are:
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.RS
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.TP
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Algorithm for constructing BWT index. BWA implements two algorithms for BWT
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construction:
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.B is
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IS linear-time algorithm for constructing suffix array. It requires
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5.37N memory where N is the size of the database. IS is moderately fast,
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but does not work with database larger than 2GB. IS is the default
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algorithm due to its simplicity. The current codes for IS algorithm are
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reimplemented by Yuta Mori.
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.TP
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.B bwtsw
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Algorithm implemented in BWT-SW. This method works with the whole human
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genome.
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.RE
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and
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.BR bwtsw .
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The first algorithm is a little faster for small database but requires large
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RAM and does not work for databases with total length longer than 2GB. The
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second algorithm is adapted from the BWT-SW source code. It in theory works
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with database with trillions of bases. When this option is not specified, the
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appropriate algorithm will be chosen automatically.
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.RE
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.TP
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@ -220,10 +221,12 @@ deducted. [5]
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Penalty for an unpaired read pair. BWA-MEM scores an unpaired read pair as
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.RI scoreRead1+scoreRead2- INT
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and scores a paired as scoreRead1+scoreRead2-insertPenalty. It compares these
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two scores to determine whether we should force pairing. [17]
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two scores to determine whether we should force pairing. A larger value leads to
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more aggressive read pair. [17]
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.TP
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.B -p
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Assume the first input query file is interleaved paired-end FASTA/Q. See the command description for details.
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Assume the first input query file is interleaved paired-end FASTA/Q. See the
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command description for details.
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.TP
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.BI -R \ STR
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Complete read group header line. '\\t' can be used in
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