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rework PE mapq; don't know if better

This commit is contained in:
Heng Li 2013-02-22 14:47:57 -05:00
parent 6c1a113753
commit ba15b787cb
2 changed files with 23 additions and 23 deletions
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1
bwamem.c
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@ -604,6 +604,7 @@ int mem_approx_mapq_se(const mem_opt_t *opt, const mem_alnreg_t *a)
int mapq, l, sub = a->sub? a->sub : opt->min_seed_len * opt->a;
double identity;
sub = a->csub > sub? a->csub : sub;
if (sub >= a->score) return 0;
l = a->qe - a->qb > a->re - a->rb? a->qe - a->qb : a->re - a->rb;
mapq = a->score? (int)(MEM_MAPQ_COEF * (1. - (double)sub / a->score) * log(a->seedcov) + .499) : 0;
identity = 1. - (double)(l * opt->a - a->score) / (opt->a + opt->b) / l;

45
bwamem_pair.c
View File

@ -14,7 +14,6 @@
#define OUTLIER_BOUND 2.0
#define MAPPING_BOUND 3.0
#define MAX_STDDEV 4.0
#define EXT_STDDEV 4.0
void bwa_hit2sam(kstring_t *str, const int8_t mat[25], int q, int r, int w, const bntseq_t *bns, const uint8_t *pac, bseq1_t *s, bwahit_t *p, int is_hard);
@ -247,7 +246,7 @@ int mem_sam_pe(const mem_opt_t *opt, const bntseq_t *bns, const uint8_t *pac, co
if (opt->flag&MEM_F_NOPAIRING) goto no_pairing;
// pairing single-end hits
if ((o = mem_pair(opt, bns->l_pac, pac, pes, s, a, id, &subo, z)) > 0) {
int is_multi[2], q_se[2], q_pe, extra_flag = 1;
int is_multi[2], q_pe, extra_flag = 1, score_un, q_se[2];
bwahit_t h[2];
// check if an end has multiple hits even after mate-SW
for (i = 0; i < 2; ++i) {
@ -257,30 +256,30 @@ int mem_sam_pe(const mem_opt_t *opt, const bntseq_t *bns, const uint8_t *pac, co
}
if (is_multi[0] || is_multi[1]) goto no_pairing; // TODO: in rare cases, the true hit may be long but with low score
// compute mapQ for the best SE hit
for (i = 0; i < 2; ++i)
q_se[i] = mem_approx_mapq_se(opt, &a[i].a[0]);
q_pe = o && subo < o? (int)(MEM_MAPQ_COEF * (1. - (double)subo / o) * log(a[0].a[z[0]].seedcov + a[1].a[z[1]].seedcov) + .499) : 0;
score_un = a[0].a[0].score + a[1].a[0].score - opt->pen_unpaired;
//q_pe = o && subo < o? (int)(MEM_MAPQ_COEF * (1. - (double)subo / o) * log(a[0].a[z[0]].seedcov + a[1].a[z[1]].seedcov) + .499) : 0;
subo = subo > score_un? subo : score_un;
q_pe = (o - subo) * 6;
if (q_pe > 60) q_pe = 60;
// the following assumes no split hits
if (z[0] == 0 && z[1] == 0) { // the best hit
q_se[0] = q_se[0] > q_pe? q_se[0] : q_pe;
q_se[1] = q_se[1] > q_pe? q_se[1] : q_pe;
extra_flag |= 2;
} else {
int un = a[0].a[0].score + a[1].a[0].score - opt->pen_unpaired;
if (o > un) { // then move the pair
int tmp[2], q_un = (o - un) * 6;
q_pe = q_pe < q_un? q_pe : q_un;
tmp[0] = q_se[0]; tmp[1] = q_se[1];
q_se[0] = z[0] == 0? q_se[0] : tmp[1] < q_pe? tmp[1] : q_pe;
q_se[1] = z[1] == 0? q_se[1] : tmp[0] < q_pe? tmp[0] : q_pe;
for (i = 0; i < 2; ++i)
if (a[i].a[z[i]].secondary >= 0)
a[i].a[z[i]].sub = a[i].a[a[i].a[z[i]].secondary].score, a[i].a[z[i]].secondary = -2;
extra_flag |= 2;
} else { // the unpaired alignment is much better
z[0] = z[1] = 0;
if (o > score_un) { // paired alignment is preferred
mem_alnreg_t *c[2];
c[0] = &a[0].a[z[0]]; c[1] = &a[1].a[z[1]];
for (i = 0; i < 2; ++i) {
if (c[i]->secondary >= 0)
c[i]->sub = a[i].a[c[i]->secondary].score, c[i]->secondary = -2;
q_se[i] = mem_approx_mapq_se(opt, c[i]);
}
q_se[0] = q_se[0] > q_pe? q_se[0] : q_pe < q_se[0] + 40? q_pe : q_se[0] + 40;
q_se[1] = q_se[1] > q_pe? q_se[1] : q_pe < q_se[1] + 40? q_pe : q_se[1] + 40;
extra_flag |= 2;
// cap at the tandem repeat score
q_se[0] = q_se[0] < (c[0]->score - c[0]->csub) * 6? q_se[0] : (c[0]->score - c[0]->csub) * 6;
q_se[1] = q_se[1] < (c[1]->score - c[1]->csub) * 6? q_se[1] : (c[1]->score - c[1]->csub) * 6;
} else { // the unpaired alignment is preferred
z[0] = z[1] = 0;
q_se[0] = mem_approx_mapq_se(opt, &a[0].a[0]);
q_se[1] = mem_approx_mapq_se(opt, &a[1].a[0]);
}
mem_alnreg2hit(&a[0].a[z[0]], &h[0]); h[0].qual = q_se[0]; h[0].flag |= 0x40 | extra_flag;
mem_alnreg2hit(&a[1].a[z[1]], &h[1]); h[1].qual = q_se[1]; h[1].flag |= 0x80 | extra_flag;