diff --git a/bwa.1 b/bwa.1 index 71fb80a..d6af7a0 100644 --- a/bwa.1 +++ b/bwa.1 @@ -56,10 +56,10 @@ Index database sequences in the FASTA format. .B -c Build color-space index. The input fast should be in nucleotide space. .TP -.B -p STR +.BI -p \ STR Prefix of the output database [same as db filename] .TP -.B -a STR +.BI -a \ STR Algorithm for constructing BWT index. Available options are: .RS .TP @@ -95,47 +95,47 @@ differences are allowed in the whole sequence. .B OPTIONS: .RS .TP 10 -.B -n NUM +.BI -n \ NUM Maximum edit distance if the value is INT, or the fraction of missing alignments given 2% uniform base error rate if FLOAT. In the latter case, the maximum edit distance is automatically chosen for different read lengths. [0.04] .TP -.B -o INT +.BI -o \ INT Maximum number of gap opens [1] .TP -.B -e INT +.BI -e \ INT Maximum number of gap extensions, -1 for k-difference mode (disallowing long gaps) [-1] .TP -.B -d INT +.BI -d \ INT Disallow a long deletion within INT bp towards the 3'-end [16] .TP -.B -i INT +.BI -i \ INT Disallow an indel within INT bp towards the ends [5] .TP -.B -l INT +.BI -l \ INT Take the first INT subsequence as seed. If INT is larger than the query sequence, seeding will be disabled. For long reads, this option is typically ranged from 25 to 35 for `-k 2'. [inf] .TP -.B -k INT +.BI -k \ INT Maximum edit distance in the seed [2] .TP -.B -t INT +.BI -t \ INT Number of threads (multi-threading mode) [1] .TP -.B -M INT +.BI -M \ INT Mismatch penalty. BWA will not search for suboptimal hits with a score lower than (bestScore-misMsc). [3] .TP -.B -O INT +.BI -O \ INT Gap open penalty [11] .TP -.B -E INT +.BI -E \ INT Gap extension penalty [4] .TP -.B -R INT +.BI -R \ INT Proceed with suboptimal alignments if there are no more than INT equally best hits. This option only affects paired-end mapping. Increasing this threshold helps to improve the pairing accuracy at the cost of speed, @@ -150,11 +150,22 @@ Disable iterative search. All hits with no more than .I maxDiff differences will be found. This mode is much slower than the default. .TP -.B -q INT +.BI -q \ INT Parameter for read trimming. BWA trims a read down to argmax_x{\\sum_{i=x+1}^l(INT-q_i)} if q_l file. .B OPTIONS: .RS .TP 10 -.B -a INT +.BI -a \ INT Score of a match [1] .TP -.B -b INT +.BI -b \ INT Mismatch penalty [3] .TP -.B -q INT +.BI -q \ INT Gap open penalty [5] .TP -.B -r INT +.BI -r \ INT Gap extension penalty. The penalty for a contiguous gap of size k is q+k*r. [2] .TP -.B -t INT +.BI -t \ INT Number of threads in the multi-threading mode [1] .TP -.B -w INT +.BI -w \ INT Band width in the banded alignment [33] .TP -.B -T INT +.BI -T \ INT Minimum score threshold divided by a [37] .TP -.B -c FLOAT +.BI -c \ FLOAT Coefficient for threshold adjustment according to query length. Given an l-long query, the threshold for a hit to be retained is a*max{T,c*log(l)}. [5.5] .TP -.B -z INT +.BI -z \ INT Z-best heuristics. Higher -z increases accuracy at the cost of speed. [1] .TP -.B -s INT +.BI -s \ INT Maximum SA interval size for initiating a seed. Higher -s increases accuracy at the cost of speed. [3] .TP -.B -N INT +.BI -N \ INT Minimum number of seeds supporting the resultant alignment to skip reverse alignment. [5] .RE @@ -361,6 +372,7 @@ _ NM Edit distance MD Mismatching positions/bases AS Alignment score +BC Barcode sequence _ X0 Number of best hits X1 Number of suboptimal hits found by BWA