FastSort/src/sort/sort.cpp

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#include "sort.h"
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#include <fcntl.h>
#include <htslib/sam.h>
#include <htslib/thread_pool.h>
#include <klib/kthread.h>
#include <math.h>
#include <pthread.h>
#include <spdlog/spdlog.h>
#include <stdio.h>
#include <stdlib.h>
#include <sys/mman.h>
#include <unistd.h>
#include <zlib.h>
#include <algorithm>
#include <string>
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#include "const_val.h"
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#include "sam_io.h"
#include "sort_args.h"
#include "sort_impl.h"
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#include "util/profiling.h"
#include "util/yarn.h"
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#include "process_header.h"
#include "phase_1.h"
#include "phase_2.h"
#include "compress.h"
#include "decompress.h"
using std::string;
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#define BAM_BLOCK_SIZE 16L * 1024 * 1024
namespace nsgv {
SortArg gSortArg; // 参数
HeaderBuf gInHdr; // 输入文件的header
bool gIsBigEndian;
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DataBuffer gMarginBuf; // 用于存放跨两个gz block的解压数据
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}; // namespace nsgv
struct BamSortData {
uint16_t flag;
uint16_t qnameLen;
uint16_t bamLen;
int32_t tid;
int64_t pos;
uint8_t *uDataPos;
char *qname; // pointer to qname
};
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/* 将bam文件内容读取到buf解析buf中的gz block长度信息 */
static size_t doFirstPipeReadFile(FirstPipeArg &p, DataBuffer &halfBlock, FILE *fpr) {
ReadBuffer &readData = p.readData[p.readOrder % p.READ_BUF_NUM];
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size_t readState = 0;
size_t curReadPos = 0;
int blockLen = 0;
int maxBlockLen = 0;
readState = fread(readData.dataBuf, 1, readData.readBufSize, fpr);
if (readState == 0) { return 0; }
readData.startAddrArr.clear();
/* 处理上一个不完整的block */ // 需要一个额外的空间来保存上一个不完整的block数据因为readbuffer里的data和block都会用来解析而且解析之后都会清空。
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if (halfBlock.readPos > 0) { // 上一轮有剩余
if (halfBlock.readPos < BLOCK_HEADER_LENGTH) { // 上一轮剩余数据不满足解析block长度信息
memcpy(&halfBlock.data[halfBlock.readPos], readData.dataBuf, BLOCK_HEADER_LENGTH - halfBlock.readPos);
halfBlock.curLen = unpackInt16(&halfBlock.data[16]) + 1; // 更新一下剩余block的真正长度
// spdlog::info("last remain, blocklen: {}, last load: {}", halfBlock.curLen, halfBlock.readPos);
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}
memcpy(readData.blockBuf, halfBlock.data, halfBlock.readPos);
curReadPos = halfBlock.curLen - halfBlock.readPos; // curlen保存上一个block的长度readPos保存上一个block在上一次读取中的长度
memcpy(&readData.blockBuf[halfBlock.readPos], readData.dataBuf, curReadPos); // 将不完整的block剩余数据拷贝到curBlock
readData.startAddrArr.push_back(readData.blockBuf);
}
/* 解析读入buf中的文件数据计算包含的每个block的长度信息和起始地址 */
while (curReadPos + BLOCK_HEADER_LENGTH <= readState) { /* 确保能解析block长度 */
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blockLen = unpackInt16(&readData.dataBuf[curReadPos + 16]) + 1;
if (blockLen > maxBlockLen) { maxBlockLen = blockLen; }
if (curReadPos + blockLen <= readState) { /* 完整的block数据在buf里 */
readData.startAddrArr.push_back(&readData.dataBuf[curReadPos]);
curReadPos += blockLen;
} else {
break; /* 当前block数据不完整一部分在还没读入的file数据里 */
}
}
/* 如果buf中包含不完整的block数据先保存一下放到下一轮里去处理 */
halfBlock.readPos = readState - curReadPos;
halfBlock.curLen = blockLen;
if (halfBlock.readPos > 0) {
memcpy(halfBlock.data, &readData.dataBuf[curReadPos], halfBlock.readPos); // 将不完整的block拷贝到halfBlock
}
// spdlog::info("block num-1: {}", readData.startAddrArr.size());
//spdlog::info("read order: {}, max block len: {}", p.readOrder, maxBlockLen);
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return readState;
}
/* FirstPipe step-1 读取文件线程 */
static void *firstPipeReadFile(void *data) {
FirstPipeArg &p = *(FirstPipeArg *)data;
/* 1. set up */
FILE *fpr = fopen(nsgv::gSortArg.INPUT_FILE.c_str(), "rb");
parseSamHeader(fpr, nsgv::gInHdr);
size_t fileSize = 0;
DataBuffer halfBlock(SINGLE_BLOCK_SIZE);
/* 2. do the work */
while (true) {
// self dependency
yarn::DEPENDENCY_NOT_TO_BE(p.readSig, p.READ_BUF_NUM);
PROF_G_BEG(read);
size_t readState = doFirstPipeReadFile(p, halfBlock, fpr);
PROF_G_END(read);
if (readState == 0) {
yarn::SIGNAL_FINISH(p.readSig, p.readFinish);
break;
}
// update self status
yarn::UPDATE_SIG_ORDER(p.readSig, p.readOrder);
fileSize += readState;
}
spdlog::info("read file order: {}, file size: {}", p.readOrder, fileSize);
/* 3. clean up */
fclose(fpr);
return nullptr;
}
static int parseBam(uint8_t* addr, bam1_t* b) {
bam1_core_t* c = &b->core;
int32_t block_len, ret, i;
uint32_t new_l_data;
uint8_t tmp[32], *x;
b->l_data = 0;
memcpy(&block_len, addr, 4);
if (nsgv::gIsBigEndian)
ed_swap_4p(&block_len);
if (block_len < 32)
return -4; // block_len includes core data
x = addr + 4;
c->tid = le_to_u32(x);
c->pos = le_to_i32(x + 4);
uint32_t x2 = le_to_u32(x + 8);
c->bin = x2 >> 16;
c->qual = x2 >> 8 & 0xff;
c->l_qname = x2 & 0xff;
c->l_extranul = (c->l_qname % 4 != 0) ? (4 - c->l_qname % 4) : 0;
uint32_t x3 = le_to_u32(x + 12);
c->flag = x3 >> 16;
c->n_cigar = x3 & 0xffff;
c->l_qseq = le_to_u32(x + 16);
c->mtid = le_to_u32(x + 20);
c->mpos = le_to_i32(x + 24);
c->isize = le_to_i32(x + 28);
#if 0
new_l_data = block_len - 32 + c->l_extranul;
if (new_l_data > INT_MAX || c->l_qseq < 0 || c->l_qname < 1)
return -4;
if (((uint64_t)c->n_cigar << 2) + c->l_qname + c->l_extranul + (((uint64_t)c->l_qseq + 1) >> 1) + c->l_qseq > (uint64_t)new_l_data)
return -4;
if (realloc_bam_data(b, new_l_data) < 0)
return -4;
b->l_data = new_l_data;
if (bgzf_read_small(fp, b->data, c->l_qname) != c->l_qname)
return -4;
if (b->data[c->l_qname - 1] != '\0') { // try to fix missing nul termination
if (fixup_missing_qname_nul(b) < 0)
return -4;
}
for (i = 0; i < c->l_extranul; ++i) b->data[c->l_qname + i] = '\0';
c->l_qname += c->l_extranul;
if (b->l_data < c->l_qname || bgzf_read_small(fp, b->data + c->l_qname, b->l_data - c->l_qname) != b->l_data - c->l_qname)
return -4;
if (fp->is_be)
swap_data(c, b->l_data, b->data, 0);
if (bam_tag2cigar(b, 0, 0) < 0)
return -4;
// TODO: consider making this conditional
if (c->n_cigar > 0) { // recompute "bin" and check CIGAR-qlen consistency
hts_pos_t rlen, qlen;
bam_cigar2rqlens(c->n_cigar, bam_get_cigar(b), &rlen, &qlen);
if ((b->core.flag & BAM_FUNMAP) || rlen == 0)
rlen = 1;
b->core.bin = hts_reg2bin(b->core.pos, b->core.pos + rlen, 14, 5);
// Sanity check for broken CIGAR alignments
if (c->l_qseq > 0 && !(c->flag & BAM_FUNMAP) && qlen != c->l_qseq) {
hts_log_error("CIGAR and query sequence lengths differ for %s", bam_get_qname(b));
return -4;
}
}
#endif
return 4 + block_len;
}
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// multi-thread uncompress bam blocks
static void mtUncompressBlock(void *data, long idx, int tid) {
PROF_T_BEG(mem_copy);
UncompressData& p = *(UncompressData*)data;
ReadBuffer &readData = *p.readDataPtr;
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auto &blockItemArr = p.blockItemArr[tid];
auto &bamItemArr = p.bamItemArr[tid];
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auto &blockItem = blockItemArr.add();
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uint8_t *block = readData.startAddrArr[idx];
size_t dlen = SINGLE_BLOCK_SIZE; // 65535
int block_length = unpackInt16(&block[16]) + 1;
uint32_t crc = le_to_u32(block + block_length - 8);
int ret = bgzfUncompress(blockItem.data, &dlen, (Bytef *)block + BLOCK_HEADER_LENGTH,
block_length - BLOCK_HEADER_LENGTH, crc);
if (ret != 0) {
spdlog::error("uncompress error, block id: {}, len: {}, ret: {}", idx, block_length, ret);
exit(0);
}
blockItem.blockId = idx;
blockItem.blockLen = dlen;
uint32_t nextBamStart = 0;
uint32_t bamLen = 0;
uint32_t bamNum = 0;
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/* 解析每个bam */
while (nextBamStart + 4 <= blockItem.blockLen) {
OneBam& bam = bamItemArr.add();
bam.blockThread = &blockItemArr;
bam.blockIdx = blockItemArr.curIdx - 1;
bam.offset = nextBamStart;
uint8_t *curAddr = &blockItem.data[nextBamStart];
#if 0
nextBamStart += parseBam(curAddr, &bam.b);
++bamNum;
#else
memcpy(&bamLen, curAddr, 4);
curAddr += 4;
if (nsgv::gIsBigEndian) ed_swap_4p(&bamLen);
bam.bamLen = bamLen;
bam.tid = le_to_u32(curAddr);
bam.pos = le_to_i32(curAddr + 4);
uint32_t x2 = le_to_u32(curAddr + 8);
bam.qnameLen = x2 & 0xff;
// spdlog::info("bam len: {}, bam struct len: {}", bamLen, sizeof(OneBam));
// spdlog::info("bam name: {}, bam name len: {}", string((char*)(blockItem.data + bam.offset + OneBam::QnameOffset), bam.qnameLen), bam.qnameLen);
nextBamStart += 4 + bamLen;
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++bamNum;
#endif
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}
// spdlog::info("bam num: {}", bamNum);
if (nextBamStart != blockItem.blockLen) {
spdlog::error("Block content does not contain integer number of bam records!");
exit(0);
}
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blockItem.bamNum = bamNum;
blockItemArr.bamNum += bamNum;
// 判断是否超过内存阈值,只要有一个超过阈值,就转入下一阶段,进行排序和合并,但是也得把这一轮数据解压处理完
if (blockItemArr.curIdx * SINGLE_BLOCK_SIZE * BLOCK_MEM_FACTOR >= p.father->singleThreadBytes) {
p.father->uncompressBufFull = 1;
} else {
// spdlog::info("block arr: {}, single thread: {}", blockItemArr.blockArr.size() * SINGLE_BLOCK_SIZE, p.father->singleThreadBytes);
}
PROF_T_END(tid, mem_copy);
}
// multi-thread uncompress bam blocks
static void mtUncompressBlockBatch(void* data, long idx, int tid) {
PROF_T_BEG(mem_copy);
UncompressData& p = *(UncompressData*)data;
ReadBuffer& readData = *p.readDataPtr;
int startIdx = START_IDX(idx, nsgv::gSortArg.NUM_THREADS, readData.startAddrArr.size());
int stopIdx = STOP_IDX(idx, nsgv::gSortArg.NUM_THREADS, readData.startAddrArr.size());
auto& blockItemArr = p.blockItemArr[tid];
auto& bamItemArr = p.bamItemArr[tid];
blockItemArr.add(stopIdx - startIdx);
for (int i = startIdx; i < stopIdx; ++i) {
auto& blockItem = blockItemArr.blockArr[blockItemArr.curIdx++];
uint8_t* block = readData.startAddrArr[i];
size_t dlen = SINGLE_BLOCK_SIZE; // 65535
int block_length = unpackInt16(&block[16]) + 1;
uint32_t crc = le_to_u32(block + block_length - 8);
int ret = bgzfUncompress(blockItem.data, &dlen, (Bytef*)block + BLOCK_HEADER_LENGTH, block_length - BLOCK_HEADER_LENGTH, crc);
if (ret != 0) {
spdlog::error("uncompress error, block id: {}, len: {}, ret: {}", idx, block_length, ret);
exit(0);
}
blockItem.blockId = i;
blockItem.blockLen = dlen;
uint32_t nextBamStart = 0;
uint32_t bamLen = 0;
uint32_t bamNum = 0;
#if 1
/* 解析每个bam */
while (nextBamStart + 4 <= blockItem.blockLen) {
bamItemArr.bamArr.push_back(OneBam());
OneBam& bam = bamItemArr.bamArr.back();
//bam.addr = &blockItem.data[nextBamStart];
uint8_t* curAddr = &blockItem.data[nextBamStart];
memcpy(&bamLen, curAddr, 4);
curAddr += 4;
if (nsgv::gIsBigEndian)
ed_swap_4p(&bamLen);
bam.bamLen = bamLen;
bam.tid = le_to_u32(curAddr);
bam.pos = le_to_i32(curAddr + 4);
uint32_t x2 = le_to_u32(curAddr + 8);
bam.qnameLen = x2 & 0xff;
//bam.qnameAddr = (char*)(curAddr + 32);
// spdlog::info("bam len: {}, bam struct len: {}", bamLen, sizeof(OneBam));
nextBamStart += 4 + bamLen;
++bamNum;
}
if (nextBamStart != blockItem.blockLen) {
spdlog::error("Block content does not contain integer number of bam records!");
exit(0);
}
#endif
blockItem.bamNum = bamNum;
}
// 判断是否超过内存阈值
if (blockItemArr.curIdx * SINGLE_BLOCK_SIZE >= p.father->singleThreadBytes) {
p.father->uncompressBufFull = 1;
} else {
// spdlog::info("block arr: {}, single thread: {}", blockItemArr.blockArr.size() * SINGLE_BLOCK_SIZE, p.father->singleThreadBytes);
}
PROF_T_END(tid, mem_copy);
}
// 线程内排序
static void mtInThreadSort(void* data, long idx, int tid) {
UncompressData& p = *(UncompressData*)data;
auto& bamItemArr = p.bamItemArr[tid];
auto& arr = bamItemArr.bamArr;
// 先按照坐标排序
#if 0
if (tid == 0)
spdlog::info("Before: {} {} {} {} {} {} {} {} {} {}", arr[0].pos, arr[1].pos, arr[2].pos, arr[3].pos, arr[4].pos, arr[5].pos, arr[6].pos,
arr[7].pos, arr[8].pos, arr[9].pos);
#endif
if (nsgv::gSortArg.SORT_COORIDINATE) {
std::sort(arr.begin(), arr.begin() + bamItemArr.curIdx, [](const OneBam& b1, const OneBam& b2) { return b1.pos < b2.pos; });
} else if (nsgv::gSortArg.QUERY_NAME_TYPE == nsmd::QueryNameType::PICARD) {
std::sort(arr.begin(), arr.begin() + bamItemArr.curIdx, [](const OneBam& b1, const OneBam& b2) {
int cmp = 0;
strncmp((char*)(b1.blockThread->blockArr[b1.blockIdx].data + b1.offset + OneBam::QnameOffset),
(char*)(b2.blockThread->blockArr[b2.blockIdx].data + b2.offset + OneBam::QnameOffset), std::min(b1.qnameLen, b2.qnameLen));
if (cmp == 0)
return b1.qnameLen < b2.qnameLen;
return cmp < 0;
});
}
#if 0
if (tid == 0)
spdlog::info("After: {} {} {} {} {} {} {} {} {} {}", arr[0].pos, arr[1].pos, arr[2].pos, arr[3].pos, arr[4].pos, arr[5].pos, arr[6].pos,
arr[7].pos, arr[8].pos, arr[9].pos);
#endif
}
// 多线程压缩
static void mtCompressBlock(void* data, long idx, int tid) {
FirstPipeArg &p = *(FirstPipeArg *)data;
auto& barr = p.sortedBamArr;
auto& t = p.taskArr[idx];
auto& buf = p.threadBuf[tid];
uint8_t block[SINGLE_BLOCK_SIZE] = {0};
uint8_t compressBlock[SINGLE_BLOCK_SIZE] = {0};
int curAddr = 0;
for (int i = t.idx; i < t.idx + t.num; ++i) {
// copy data
memccpy(&block[curAddr], barr[i]->blockThread->blockArr[barr[i]->blockIdx].data + barr[i]->offset, 1, barr[i]->bamLen);
curAddr += barr[i]->bamLen;
}
size_t dlen = BGZF_MAX_BLOCK_SIZE;
// spdlog::info("len: {}", curAddr);
bgzfCompress(block, &dlen, compressBlock, curAddr, -1);
// 先放到自己线程内部的buf里
// 然后在压缩完成之后检测当前全局压缩序号如果当前序号的block在自己线程内则复制过去
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}
/* 将gz block进行解压并进行线程内排序 */
static void doFirstPipeUncompress(FirstPipeArg &p, int finish = 0) {
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// return;
PROF_G_BEG(uncompress);
ReadBuffer &readData = p.readData[p.uncompressOrder % p.READ_BUF_NUM];
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UncompressData &uncompressData = p.uncompressData[p.uncompressOrder % p.UNCOMPRESS_BUF_NUM];
uncompressData.readDataPtr = &readData;
#if 1
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kt_for(p.numThread, mtUncompressBlock, &uncompressData, readData.startAddrArr.size());
#else
kt_for(p.numThread, mtUncompressBlockBatch, &uncompressData, nsgv::gSortArg.NUM_THREADS);
#endif
PROF_G_END(uncompress);
// 判断是否超过内存阈值,只要有一个超过阈值,就转入下一阶段,进行排序和合并
if (p.uncompressBufFull == 1 || finish) {
spdlog::info("buf full - {}", p.mergeOrder);
// spdlog::info("max mem: {}, single thread mem: {}", nsgv::gSortArg.MAX_MEM, p.singleThreadBytes);
// sort排序的时候应该线程利用率很高此时不需要跟其他操作如压缩等进行重叠了
PROF_G_BEG(sort);
kt_for(p.numThread, mtInThreadSort, &uncompressData, nsgv::gSortArg.NUM_THREADS);
PROF_G_END(sort);
// merge
#if 0
auto& bamArr = p.sortedBamArr;
BamHeap<BamGreaterThan> heap;
heap.Init(&uncompressData.bamItemArr);
bamArr.resize(heap.Size());
auto& taskArr = p.taskArr;
taskArr.clear();
const OneBam* bam = nullptr;
uint64_t posAll = 0;
int i = 0;
ArrayInterval intv{0, 0};
uint64_t blockBytes = 0;
PROF_G_BEG(merge);
while ((bam = heap.Pop()) != nullptr) {
// posAll += bam->pos;
//spdlog::info("pos: {}", bam->pos);
bamArr[i++] = bam;
blockBytes += bam->bamLen;
if (blockBytes >= SINGLE_BLOCK_SIZE) {
intv.num = i - 1 - intv.idx;
taskArr.push_back(intv);
intv.idx = i - 1;
blockBytes = bam->bamLen;
}
}
PROF_G_END(merge);
spdlog::info("task size: {} {}", taskArr.size(), bamArr.size());
if (i - 1 > intv.idx) {
intv.num = i - 1 - intv.idx;
taskArr.push_back(intv);
}
// spdlog::info("pos all: {}", posAll);
#endif
// compress此时并行压缩的多线程利用率应该很高了
PROF_G_BEG(compress);
#if 0
kt_for(p.numThread, mtCompressBlock, &p, taskArr.size());
#endif
for (auto &blockItemArr : uncompressData.blockItemArr) {
p.bamNum += blockItemArr.bamNum;
}
uncompressData.ResetBlockArr();
uncompressData.ResetBamArr();
p.uncompressBufFull = 0;
p.mergeOrder++;
for(auto &buf : p.threadBuf) {
buf.curLen = 0;
}
PROF_G_END(compress);
// 压缩完之后应该写入中间文件这个应该可以overlap
}
// 等处理完这些数据之后,进入新一轮的解压和归并排序压缩输出中间文件。
// auto &bam = uncompressData.bamItemArr[0].bamArr.back();
// string qname(bam.qnameAddr, bam.qnameLen);
// spdlog::info("bam name:{}", qname);
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}
/* FirstPipe step-2 并行解压gz blocks*/
static void *firstPipeUncompress(void *data) {
FirstPipeArg &p = *(FirstPipeArg *)data;
/* 2. do the work */
while (true) {
// previous dependency
yarn::DEPENDENCY_NOT_TO_BE(p.readSig, 0);
// self dependency
yarn::DEPENDENCY_NOT_TO_BE(p.uncompressSig, p.UNCOMPRESS_BUF_NUM);
if (p.readFinish) {
while (p.uncompressOrder < p.readOrder) {
yarn::DEPENDENCY_NOT_TO_BE(p.uncompressSig, p.UNCOMPRESS_BUF_NUM);
doFirstPipeUncompress(p, 1);
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yarn::UPDATE_SIG_ORDER(p.uncompressSig, p.uncompressOrder);
}
yarn::SIGNAL_FINISH(p.uncompressSig, p.uncompressFinish);
break;
}
doFirstPipeUncompress(p);
// update status
yarn::CONSUME_SIGNAL(p.readSig);
yarn::UPDATE_SIG_ORDER(p.uncompressSig, p.uncompressOrder);
}
spdlog::info("uncompress order: {}", p.uncompressOrder);
return nullptr;
}
/* 将并行解压的数据放到一起,解析,到容量阈值后,进行排序并输出到中间文件 */
void dofirstPipeWrite(FirstPipeArg &p) {
PROF_G_BEG(sort);
UncompressData &uncompressData = p.uncompressData[p.writeOrder % p.UNCOMPRESS_BUF_NUM];
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size_t blockNum = 0;
for (int i = 0; i < p.numThread; ++i) {
blockNum += uncompressData.blockItemArr[i].curIdx;
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}
size_t bamNum = blockNum * 256;
size_t curBlockBamMemSize = blockNum * SINGLE_BLOCK_SIZE + bamNum * 32;
/* 内存使用量达到阈值后,进行排序 */
if (curBlockBamMemSize > nsgv::gSortArg.MAX_MEM / 2) {
//uncompressData.ResetBlockArr();
//uncompressData.ResetBamArr();
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}
//spdlog::info("block num: {}, bam num: {}, block size: {}, bam size: {}", blockNum, bamNum, blockBytes, bamNum * 32);
// spdlog::info("block num: {}, bam num: {}, block size: {}, bam size: {}", blockNum, bamNum, blockNum * SINGLE_BLOCK_SIZE, bamNum * 32);
PROF_G_END(sort);
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}
/* FirstPipe step-3 串行写入中间文件(已经压缩好) */
static void *firstPipeWrite(void *data) {
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FirstPipeArg &p = *(FirstPipeArg *)data;
while (true) {
yarn::DEPENDENCY_NOT_TO_BE(p.uncompressSig, 0);
if (p.uncompressFinish) {
spdlog::info("uncompress finish, cur sort order: {}", p.writeOrder);
while (p.writeOrder < p.uncompressOrder) {
dofirstPipeWrite(p);
p.writeOrder += 1;
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}
/* 需要检测一下buf中是否还有数据如果还有则需要进行排序可以不输出到中间文件直接进行second-pipe的归并排序 */
break;
}
dofirstPipeWrite(p);
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p.writeOrder += 1;
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// update status
yarn::CONSUME_SIGNAL(p.uncompressSig);
}
spdlog::info("merge sort order: {}", p.writeOrder);
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return nullptr;
}
/* 对bam文件进行排序第一阶段线程内排序线程间merge输入到中间bam文件 */
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static void bamSortFirstPipe() {
/* set up*/
FirstPipeArg firstPipeArg;
firstPipeArg.numThread = nsgv::gSortArg.NUM_THREADS;
firstPipeArg.singleThreadBytes = nsgv::gSortArg.MAX_MEM / firstPipeArg.numThread;
spdlog::info("max mem: {}, single thread mem: {}", nsgv::gSortArg.MAX_MEM, firstPipeArg.singleThreadBytes);
const size_t kReadBufSize = 1L * 1024 * 1024 * firstPipeArg.numThread; // 平均每线程1M缓冲区累加起来用来读入文件BAM/SAM相对解压之后的缓冲区大小可以忽略
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for (int i = 0; i<firstPipeArg.READ_BUF_NUM; ++i) {
firstPipeArg.readData[i].Resize(kReadBufSize);
}
// 根据最大内存参数,计算初始化开辟的空间
int blockInitNum = nsgv::gSortArg.MAX_MEM / nsgv::gSortArg.NUM_THREADS / SINGLE_BLOCK_SIZE / BLOCK_MEM_FACTOR;
spdlog::info("Init block num: {}", blockInitNum);
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for (int i = 0; i < firstPipeArg.UNCOMPRESS_BUF_NUM; ++i) {
firstPipeArg.uncompressData[i].Resize(firstPipeArg.numThread, blockInitNum);
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}
PROF_G_BEG(mid_all);
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/* create threads */
pthread_t pipeThreadIdArr[3]; // 3-stage pipeline
pthread_create(&pipeThreadIdArr[0], NULL, firstPipeReadFile, &firstPipeArg);
pthread_create(&pipeThreadIdArr[1], NULL, firstPipeUncompress, &firstPipeArg);
pthread_create(&pipeThreadIdArr[2], NULL, firstPipeWrite, &firstPipeArg);
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for (int i = 0; i < 3; ++i) pthread_join(pipeThreadIdArr[i], NULL);
spdlog::info("all bams num: {}", firstPipeArg.bamNum);
PROF_G_END(mid_all);
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}
/* IO同步的方式进行排序 */
static void bamSortSerialFirstPipe() {
/* set up*/
FirstPipeArg firstPipeArg;
firstPipeArg.numThread = nsgv::gSortArg.NUM_THREADS;
const size_t kReadBufSize = 1L * 1024 * 1024 * firstPipeArg.numThread;
for (int i = 0; i < firstPipeArg.READ_BUF_NUM; ++i) {
firstPipeArg.readData[i].Resize(kReadBufSize);
}
for (int i = 0; i < firstPipeArg.UNCOMPRESS_BUF_NUM; ++i) {
firstPipeArg.uncompressData[i].Resize(firstPipeArg.numThread, 128);
}
/* 1. read file */
FILE *fpr = fopen(nsgv::gSortArg.INPUT_FILE.c_str(), "rb");
parseSamHeader(fpr, nsgv::gInHdr);
size_t fileSize = 0;
DataBuffer halfBlock(SINGLE_BLOCK_SIZE);
/* 2. do the work */
while (true) {
size_t readState = doFirstPipeReadFile(firstPipeArg, halfBlock, fpr);
doFirstPipeUncompress(firstPipeArg);
dofirstPipeWrite(firstPipeArg);
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if (readState == 0) break;
fileSize += readState;
}
/* 3. clean up */
fclose(fpr);
}
/* 对sam文件进行排序 */
static void samSortFirstPipe() {
}
// 排序的入口函数entry function
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int doSort() {
#if 1
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nsgv::gIsBigEndian = ed_is_big();
// 第一轮排序,外排到多个中间文件
// bamSortFirstPipe();
phase1Pipeline();
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spdlog::info("OneBam size: {}", sizeof(OneBam));
// bamSortSerialFirstPipe();
#else
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/* 打开输入bam文件 */
samFile *inBamFp = sam_open_format(nsgv::gSortArg.INPUT_FILE.c_str(), "r", nullptr);
if (!inBamFp) {
spdlog::error("[{}] load sam/bam file failed.\n", __func__);
return -1;
}
hts_set_opt(inBamFp, HTS_OPT_BLOCK_SIZE, BAM_BLOCK_SIZE);
sam_hdr_t *inBamHdr = sam_hdr_read(inBamFp); // 读取header
htsThreadPool htsPoolRead = {NULL, 0}; // 多线程读取,创建线程池
htsPoolRead.pool = hts_tpool_init(nsgv::gSortArg.NUM_THREADS);
if (!htsPoolRead.pool) {
spdlog::error("[{}] failed to set up thread pool", __LINE__);
sam_close(inBamFp);
return -1;
}
hts_set_opt(inBamFp, HTS_OPT_THREAD_POOL, &htsPoolRead);
// 测试读取bam的速度
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int max_bam_len = 0;
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bam1_t *bamp = bam_init1();
uint64_t bam_num = 0;
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while (sam_read1(inBamFp, inBamHdr, bamp) >= 0) {
bam_num++;
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if (max_bam_len < bamp->l_data) max_bam_len = bamp->l_data;
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if (bamp->l_data > 1000) {
spdlog::info("large record len: {}", bamp->l_data);
}
}
sam_close(inBamFp);
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spdlog::info("max record len: {}", max_bam_len);
spdlog::info("bam num: {}", bam_num);
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#endif
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return 0;
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}