FastSort/ext/htslib/samples/index_multireg_read.c

/*  index_multireg_read.c --  showcases the htslib api usage

    Copyright (C) 2023 Genome Research Ltd.

    Author: Vasudeva Sarma <vasudeva.sarma@sanger.ac.uk>

Permission is hereby granted, free of charge, to any person obtaining a copy
of this software and associated documentation files (the "Software"), to deal
in the Software without restriction, including without limitation the rights
to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
copies of the Software, and to permit persons to whom the Software is
furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in
all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL
THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
DEALINGS IN THE SOFTWARE

*/

/* The purpose of this code is to demonstrate the library apis and need proper error handling and optimisation */

#include <getopt.h>
#include <unistd.h>
#include <htslib/sam.h>

/// print_usage - print the usage
/** @param fp pointer to the file / terminal to which usage to be dumped
returns nothing
*/
static void print_usage(FILE *fp)
{
    fprintf(fp, "Usage: read_multireg infile count regspec_csv\n\
    Reads alignment of a target matching to given region specifications\n\
    read_multireg infile.sam 2 R1:10-100,R2:200");
    return;
}

/// main_demo - start of the demo
/** @param argc - count of arguments
 *  @param argv - pointer to array of arguments
returns 1 on failure 0 on success
*/
int main(int argc, char *argv[])
{
    const char *inname = NULL;
    char *ptr = NULL;
    int c = 0, ret = EXIT_FAILURE;
    samFile *infile = NULL, *outfile = NULL;
    sam_hdr_t *in_samhdr = NULL;
    bam1_t *bamdata = NULL;
    hts_idx_t *idx = NULL;
    hts_itr_t *iter = NULL;
    unsigned int regcnt = 0;
    char **regions = NULL;

    //read_multireg infile count regspec_csv
    if (argc != 4) {
        print_usage(stderr);
        goto end;
    }
    inname = argv[1];
    regcnt = atoi(argv[2]);
    regions = calloc(regcnt, sizeof(char*));
    //set each regspec as separate entry in region array
    ptr = argv[3];
    for (c = 0; ptr && (c < regcnt); ++c) {
        regions[c] = ptr;
        ptr = strchr(ptr, ',');
        if (ptr) { *ptr = '\0'; ++ptr; }
    }

    if (regcnt == 0) {
        printf("Region count can not be 0\n");
        goto end;
    }
    //initialize bam data storage
    if (!(bamdata = bam_init1())) {
        printf("Failed to initialize bamdata\n");
        goto end;
    }
    //open files, use stdout as output SAM file for ease of display
    infile = sam_open(inname, "r");
    outfile = sam_open("-", "w");
    if (!outfile || !infile) {
        printf("Could not open in/out files\n");
        goto end;
    }
    //load index file, assume it to be present in same location
    if (!(idx = sam_index_load(infile, inname))) {
        printf("Failed to load the index\n");
        goto end;
    }
    //read header
    if (!(in_samhdr = sam_hdr_read(infile))) {
        printf("Failed to read header from file!\n");
        goto end;
    }
    //create iterator
    if (!(iter = sam_itr_regarray(idx, in_samhdr, regions, regcnt))) {
        printf("Failed to get iterator\n");
        goto end;
    }
    if (regions) {
        //can be freed as it is no longer required
        free(regions);
        regions = NULL;
    }

    //get required area
    while ((c = sam_itr_multi_next(infile, iter, bamdata) >= 0)) {
        //write to output
        if (sam_write1(outfile, in_samhdr, bamdata) < 0) {
            printf("Failed to write output\n");
            goto end;
        }
    }
    if (c != -1) {
        printf("Error during read\n");
        goto end;
    }
    ret = EXIT_SUCCESS;

end:
    //cleanup
    if (in_samhdr) {
        sam_hdr_destroy(in_samhdr);
    }
    if (infile) {
        sam_close(infile);
    }
    if (outfile) {
        sam_close(outfile);
    }
    if (bamdata) {
        bam_destroy1(bamdata);
    }
    if (iter) {
        sam_itr_destroy(iter);
    }
    if (idx)
        hts_idx_destroy(idx);
    return ret;
}