167 lines
5.2 KiB
C
167 lines
5.2 KiB
C
/* index_write.c -- showcases the htslib api usage
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Copyright (C) 2023 Genome Research Ltd.
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Author: Vasudeva Sarma <vasudeva.sarma@sanger.ac.uk>
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Permission is hereby granted, free of charge, to any person obtaining a copy
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of this software and associated documentation files (the "Software"), to deal
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in the Software without restriction, including without limitation the rights
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to use, copy, modify, merge, publish, distribute, sublicense, and/or sell
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copies of the Software, and to permit persons to whom the Software is
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furnished to do so, subject to the following conditions:
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The above copyright notice and this permission notice shall be included in
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all copies or substantial portions of the Software.
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THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR
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IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY,
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FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL
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THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER
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LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING
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FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER
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DEALINGS IN THE SOFTWARE
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*/
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/* The purpose of this code is to demonstrate the library apis and need proper error handling and optimisation */
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#include <getopt.h>
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#include <libgen.h>
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#include <unistd.h>
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#include <htslib/sam.h>
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/// print_usage - print the usage
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/** @param fp pointer to the file / terminal to which usage to be dumped
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returns nothing
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*/
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static void print_usage(FILE *fp)
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{
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fprintf(fp, "Usage: idx_on_write infile shiftsize outdir\n\
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Creates compressed sam file and index file for it in given directory\n");
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return;
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}
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/// main_demo - start of the demo
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/** @param argc - count of arguments
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* @param argv - pointer to array of arguments
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returns 1 on failure 0 on success
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*/
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int main(int argc, char *argv[])
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{
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const char *outdir = NULL;
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char *inname = NULL, *fileidx = NULL, *outname = NULL, outmode[4] = "w";
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int c = 0, ret = EXIT_FAILURE, size = 0;
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samFile *infile = NULL, *outfile = NULL;
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sam_hdr_t *in_samhdr = NULL;
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bam1_t *bamdata = NULL;
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//idx_on_write infile sizeshift outputdirectory
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if (argc != 4) {
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print_usage(stderr);
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goto end;
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}
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inname = argv[1];
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size = atoi(argv[2]);
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outdir = argv[3];
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//allocate space for output name - outdir/filename.ext.idxextNUL
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c = strlen(basename(inname)) + strlen(outdir) + 10;
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fileidx = malloc(sizeof(char) * c);
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outname = malloc(sizeof(char) * c);
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if (!fileidx || !outname) {
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printf("Couldnt allocate memory\n");
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goto end;
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}
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//initialize bam storage
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if (!(bamdata = bam_init1())) {
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printf("Failed to initialize bamdata\n");
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goto end;
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}
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//open files
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if ((infile = sam_open(inname, "r"))) {
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//get file type and create output names
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if (infile->format.format == cram) {
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//set as crai
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snprintf(fileidx, c, "%s/%s.crai", outdir, basename(inname));
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snprintf(outname, c, "%s/%s", outdir, basename(inname));
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}
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else {
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//set as either bai or csi based on interval
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if (infile->format.format == sam && infile->format.compression == no_compression) {
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//create as gzip compressed
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snprintf(outname, c, "%s/%s.gz", outdir, basename(inname));
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snprintf(fileidx, c, "%s/%s.gz.%s", outdir, basename(inname), !size ? "bai" : "csi");
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}
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else {
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//with same name as input
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snprintf(outname, c, "%s/%s", outdir, basename(inname));
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snprintf(fileidx, c, "%s/%s.%s", outdir, basename(inname), !size ? "bai" : "csi");
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}
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}
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}
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c = 0;
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sam_open_mode(outmode + 1, outname, NULL); //set extra write options based on name
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outfile = sam_open(outname, outmode);
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if (!outfile || !infile) {
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printf("Could not open files\n");
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goto end;
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}
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//read header
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if (!(in_samhdr = sam_hdr_read(infile))) {
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printf("Failed to read header from file!\n");
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goto end;
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}
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//write header
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if (sam_hdr_write(outfile, in_samhdr)) {
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printf("Failed to write header\n");
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goto end;
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}
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// initialize indexing, before start of write
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if (sam_idx_init(outfile, in_samhdr, size, fileidx)) {
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printf("idx initialization failed\n");
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goto end;
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}
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//read and write alignments
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while ((c = sam_read1(infile, in_samhdr, bamdata)) >= 0) {
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if (sam_write1(outfile, in_samhdr, bamdata) < 0) {
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printf("Failed to write data\n");
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goto end;
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}
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}
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if (c != -1) {
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printf("Error in reading data\n");
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goto end;
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}
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//else EOF, save index
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if (sam_idx_save(outfile)) {
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printf("Could not save index\n");
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goto end;
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}
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ret = EXIT_SUCCESS;
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end:
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//cleanup
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if (in_samhdr) {
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sam_hdr_destroy(in_samhdr);
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}
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if (infile) {
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sam_close(infile);
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}
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if (bamdata) {
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bam_destroy1(bamdata);
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}
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if (fileidx) {
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free(fileidx);
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}
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if (outname) {
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free(outname);
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}
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if (outfile) {
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sam_close(outfile);
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}
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return ret;
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}
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