/* write_fast.c -- showcases the htslib api usage Copyright (C) 2023 Genome Research Ltd. Author: Vasudeva Sarma Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions: The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software. THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE */ /* The purpose of this code is to demonstrate the library apis and need proper error handling and optimisation */ #include #include #include #include #include /// print_usage - show usage /** @param fp pointer to the file / terminal to which usage to be dumped returns nothing */ static void print_usage(FILE *fp) { fprintf(fp, "Usage: write_fast [ 4 || argc < 3) { print_usage(stdout); goto end; } outname = argv[1]; data = argv[2]; if (argc == 4) { //fastq data qual = argv[3]; if (strlen(data) != strlen(qual)) { //check for proper length of data and quality values printf("Incorrect reference and quality data\n"); goto end; } } //initialize if (!(bamdata = bam_init1())) { printf("Failed to initialize bamdata\n"); goto end; } if (sam_open_mode(mode + 1, outname, NULL) < 0) { printf("Invalid file name\n"); goto end; } //open output file if (!(outfile = sam_open(outname, mode))) { //expects the name to have correct extension! printf("Could not open %s\n", outname); goto end; } /* if the file name extension is not appropriate to the content, inconsistent data will be present in output. if required, htsFormat and sam_open_format can be explicitly used to ensure appropriateness of content. htsFormat fmt = {sequence_data, fastq_format / fasta_format}; sam_open_format(outname, mode, fmt); */ snprintf(name, sizeof(name), "Test_%ld", (long) time(NULL)); //data if (bam_set1(bamdata, strlen(name), name, BAM_FUNMAP, -1, -1, 0, 0, NULL, -1, -1, 0, strlen(data), data, qual, 0) < 0) { printf("Failed to set data\n"); goto end; } //as we write only FASTA/FASTQ, we can get away without providing headers if (sam_write1(outfile, NULL, bamdata) < 0) { printf("Failed to write data\n"); goto end; } ret = EXIT_SUCCESS; end: //clean up if (outfile) { sam_close(outfile); } if (bamdata) { bam_destroy1(bamdata); } return ret; }