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zzh 2025-04-23 15:02:54 +08:00
commit 6175ce9a83
1 changed files with 9 additions and 17 deletions

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Identifies duplicate reads. This tool locates and tags duplicate reads in a coordinate ordered SAM or BAM file. Identifies duplicate reads. This tool locates and tags duplicate reads in a coordinate ordered SAM or BAM file.
Use the same algorithm as picard MarkDuplicates and output identical results. Use the same algorithm as picard MarkDuplicates and output identical results, and use spdlog as log tool and the default level is 'info'.
Use spdlog as log tool and the default level is 'info'.
### Features ### Features
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## Requirements ## Requirements
### Build tools Install following tools and required libraries.
* autoconf (for htslib) ```bash
* cmake # install autoconf (for htslib), cmake, c++17 (gcc >= 8.1 or clang >= 7 should work), zlib, libbz2, liblzma, libcurl, libdeflate (optional)
* c++17 (gcc >= 8.1 or clang >= 7 should work.) sudo apt update
sudo apt install autoconf cmake g++-8 zlib1g-dev libbz2-dev liblzma-dev libcurl4-openssl-dev libdeflate-dev gcc-8 g++-8
### Libraries needed ```
* zlib
* libbz2
* liblzma
* libcurl
* OpenSSL
* libdeflate (optional)
## Install ## Install
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## Usage ## Usage
1. get help Get help
```bash ```bash
./fastdup --help ./fastdup --help
``` ```
2. mark duplicates on an input BAM file using 8 threads Mark duplicates on an input BAM file using 8 threads
```bash ```bash
./fastdup --input in_test.bam --output out_md.bam --metrics stats.txt --num-threads 8 ./fastdup --input in_test.bam --output out_md.bam --metrics stats.txt --num-threads 8