改了几个bug，比如gen_sam需要全局id，dedup-sort需要一个一个做，现在还有一个问题，就是dedup-sort之后有些skip结果会变

.gitignore

@@ -1,6 +1,7 @@
 *.log
 *.sam
 output/
+scripts/
 
 # Prerequisites
 *.d

.vscode/launch.json

@@ -18,10 +18,10 @@
                 "-R",
                 "'@RG\\tID:normal\\tSM:normal\\tPL:illumina\\tLB:normal\\tPG:bwa'",
                 "~/data/reference/fmt/human_g1k_v37_decoy.fasta",
-                "~/data/dataset/D2/n1.fq.gz",
+                //"~/data/dataset/D2/n1.fq.gz", "~/data/dataset/D2/n2.fq.gz",
-                "~/data/dataset/D2/n2.fq.gz",
+                "~/data/dataset/D2/d1.fq", "~/data/dataset/D2/d2.fq",
                 "-o",
-                "D1-out.sam",
+                "/dev/null", // "D1-out.sam",
             ],
             "cwd": "${workspaceFolder}", // 当前工作路径：当前文件所在的工作空间
         },

Makefile

@@ -1,5 +1,5 @@
 CC=			gcc
-CFLAGS=		-g -Wall -Wno-unused-function -mavx2 -mavx512bw -O3
+CFLAGS=		-g -Wall -Wno-unused-function -mavx2 -mavx512bw #-O3
 WRAP_MALLOC=-DUSE_MALLOC_WRAPPERS
 
 SHOW_PERF= -DSHOW_PERF

paired_sam.c

@@ -579,7 +579,13 @@ static void workder_gen_sam(void* data, int idx, int tid) {
         mem_alnreg_v* a = &w->regs[id];
         seq_sam_t* ss = &w->sams[id];
 
-        // int cmp = strcmp("ERR194147.1548", s[0].name);
+        int cmp = strcmp("ERR194147.42331121", s[0].name) == 0;
+        // int cmp = (id + w->n_processed) == 202924;
+        if (cmp == 1) {
+            fprintf(stderr, "%s\n", s[0].name);
+            fflush(stderr);
+            //exit(0);
+        }
 
         int n[2] = {0}, nn[2] = {0};
         for (i = 0; i < 2; ++i) {
@@ -600,37 +606,49 @@ static void workder_gen_sam(void* data, int idx, int tid) {
                             skip |= 1 << r;
                     }
                 }
-                if ((t->skip | skip) != 15)  { // 检查新添加的aln是不是把当前的任务skip掉了
+                //if ((t->skip | skip) != 15)  { // 检查新添加的aln是不是把当前的任务skip掉了
-                    kswr_avx_t aln = t->aln; fprintf(gf[0], "id-%ld score-%d te-%d qe-%d score2-%d te2-%d tb-%d qb-%d\n", s[si].id +
+                    kswr_avx_t aln = t->aln; // fprintf(gf[0], "id-%ld score-%d te-%d qe-%d score2-%d te2-%d tb-%d qb-%d\n", s[si].id + w->n_processed, aln.score, aln.te, aln.qe, aln.score2, aln.te2, aln.tb, aln.qb);
-                    w->n_processed, aln.score, aln.te, aln.qe, aln.score2, aln.te2, aln.tb, aln.qb);
+                    if (cmp) {
+                        fprintf(stderr, "id-%ld score-%d te-%d qe-%d score2-%d te2-%d tb-%d qb-%d\n", s[si].id + w->n_processed, aln.score, aln.te, aln.qe, aln.score2, aln.te2, aln.tb, aln.qb);
+                        fflush(stderr);
+                    }
                     nn[si] += 1;
                     n[si] += check_add_align(t->aln, opt->min_seed_len, t->is_rev, bns->l_pac, b, s[si].l_seq, (uint8_t*)s[si].seq, ma, t->rb);
-                }
+                //}
             }
         }
         // 处理完2个pair read之后再排序，因为上面操作有插入，排序后之前记录的索引就无效了，所以上面不能排序，要两个pair read处理完之后再排序
         for (i = 0; i < 2; ++i) {
             int tmp;
             if (nn[i] > 0) {
+                int origin_n = a[i].n;
+                int start_n = a[i].n - n[i];
                 for (j = 0; j < n[i]; ++j) {  // 在这里排序
                     // move b s.t. ma is sorted
-                    int nidx = a[i].n - n[i] + j;
+                    int nidx = origin_n - n[i] + j;
                     mem_alnreg_t b = a[i].a[nidx]; // 用指针一定要慎重，注意指向的地址内容会变
                     for (k = 0; k < nidx; ++k)  // find the insertion point
                         if (a[i].a[k].score < b.score)
                             break;
                     tmp = k;
-                    for (k = nidx; k > tmp; --k) a[i].a[k] = a[i].a[k - 1];
+                    for (k = start_n; k > tmp; --k) a[i].a[k] = a[i].a[k - 1];
                     a[i].a[k] = b;
+                    // 每添加一个aln都要sort-dedup一次
+#if 1
+                    start_n = mem_sort_dedup_patch(opt, 0, 0, 0, start_n + 1, a[i].a);
+                    a[i].n = start_n;
+#else
+                    start_n += 1;
+#endif
                 }
 
-                a[i].n = mem_sort_dedup_patch(opt, 0, 0, 0, a[i].n, a[i].a);
+                // a[i].n = mem_sort_dedup_patch(opt, 0, 0, 0, a[i].n, a[i].a);
             }
         }
 
         ////////////////////////////////////////////////////////////
-
+        // 这里需要传入全局id
-        generate_sam(opt, bns, pac, pes, id >> 1, s, a, ss, w->n_processed, tid);
+        generate_sam(opt, bns, pac, pes, (w->n_processed + id) >> 1, s, a, ss, w->n_processed, tid);
     }
 }
 

run.sh

@@ -2,16 +2,20 @@ thread=1
 
 make clean; make -j 32
 
-n1=~/data/dataset/D2/n1.fq.gz
+#n1=~/data/dataset/D2/n1.fq.gz
-n2=~/data/dataset/D2/n2.fq.gz
+#n2=~/data/dataset/D2/n2.fq.gz
+n1=~/data/dataset/D2/d1.fq
+n2=~/data/dataset/D2/d2.fq
 
 #n1=/file-data/zzh/dataset/D1/45mr1.fq.gz
 #n2=/file-data/zzh/dataset/D1/45mr2.fq.gz
 
 reference=~/data/reference/fmt/human_g1k_v37_decoy.fasta
 
-out=/dev/null
+#out=/dev/null
 #out=./oldsam-D2-out.sam
+#out=./D2-1.sam
+out=./d.sam
 prog=./fastalign
 #prog=/home/zzh/fastbwa