diff --git a/bntseq.c b/bntseq.c
index 0322040..188763b 100644
--- a/bntseq.c
+++ b/bntseq.c
@@ -424,7 +424,7 @@ uint8_t *bns_get_seq(int64_t l_pac, const uint8_t *pac, int64_t beg, int64_t end
 	return seq;
 }
 
-void bns_get_seq_no_alloc(int64_t l_pac, const uint8_t *pac, int64_t beg, int64_t end, int64_t *len, size_t *m_seq, uint8_t **seqp)
+void bns_get_seq_no_alloc(int64_t l_pac, const uint8_t *pac, int64_t beg, int64_t end, size_t *len, size_t *m_seq, uint8_t **seqp)
 {
 	if (end < beg) end ^= beg, beg ^= end, end ^= beg; // if end is smaller, swap
 	if (end > l_pac<<1) end = l_pac<<1;
@@ -477,8 +477,9 @@ uint8_t *bns_fetch_seq(const bntseq_t *bns, const uint8_t *pac, int64_t *beg, in
 
 void bns_fetch_seq_no_alloc(const bntseq_t *bns, const uint8_t *pac, int64_t *beg, int64_t mid, int64_t *end, int *rid, size_t *m_seq, uint8_t **seqp)
 {
-	int64_t far_beg, far_end, len;
-	int is_rev;
+    int64_t far_beg, far_end;
+    size_t len;
+    int is_rev;
 
 	if (*end < *beg) *end ^= *beg, *beg ^= *end, *end ^= *beg; // if end is smaller, swap
 	assert(*beg <= mid && mid < *end);
diff --git a/bntseq.h b/bntseq.h
index 7ea0286..e0ae040 100644
--- a/bntseq.h
+++ b/bntseq.h
@@ -79,9 +79,11 @@ extern "C" {
 	uint8_t *bns_get_seq(int64_t l_pac, const uint8_t *pac, int64_t beg, int64_t end, int64_t *len);
 	uint8_t *bns_fetch_seq(const bntseq_t *bns, const uint8_t *pac, int64_t *beg, int64_t mid, int64_t *end, int *rid);
 	int bns_intv2rid(const bntseq_t *bns, int64_t rb, int64_t re);
-	void bns_fetch_seq_no_alloc(const bntseq_t *bns, const uint8_t *pac, int64_t *beg, int64_t mid, int64_t *end, int *rid, size_t *m_seq, uint8_t **seqp);
+    void bns_get_seq_no_alloc(int64_t l_pac, const uint8_t* pac, int64_t beg, int64_t end, size_t* len, size_t* m_seq, uint8_t** seqp);
+    void bns_fetch_seq_no_alloc(const bntseq_t* bns, const uint8_t* pac, int64_t* beg, int64_t mid, int64_t* end, int* rid, size_t* m_seq,
+                                uint8_t** seqp);
 #ifdef __cplusplus
-}
+    }
 #endif
 
 static inline int64_t bns_depos(const bntseq_t *bns, int64_t pos, int *is_rev)
diff --git a/bwamem.c b/bwamem.c
index b169cb0..eabf10c 100644
--- a/bwamem.c
+++ b/bwamem.c
@@ -111,7 +111,8 @@ mem_opt_t *mem_opt_init()
 	o->max_chain_extend = 1<<30;
 	o->mapQ_coef_len = 50; o->mapQ_coef_fac = log(o->mapQ_coef_len);
 	bwa_fill_scmat(o->a, o->b, o->mat);
-	return o;
+    o->msw_batch_size = 1024;
+    return o;
 }
 
 /***************************
@@ -1293,6 +1294,16 @@ mem_worker_t *init_mem_worker(const mem_opt_t *opt, const FMTIndex *fmt, const b
 	w->matesw_arr16 = malloc(i * sizeof(matesw_data_v*));
     w->msw_buf = calloc(i, sizeof(matesw_buf_t));
     w->mem_stats = calloc(i, sizeof(mem_stats_t));
+    w->msw_2_arr8 = malloc(i * sizeof(matesw_ptr_v*));
+    w->msw_ref = calloc(i, sizeof(byte_vv));
+    w->msw_seq = calloc(i, sizeof(msw_seq_v));
+    w->msw_kswr = calloc(i, sizeof(msw_kswr_v));
+    w->msw_xtra = calloc(i, sizeof(int_v));
+    
+	w->msw_2_ref = calloc(i, sizeof(msw_ref_v));
+    w->msw_2_seq = calloc(i, sizeof(msw_seq_v));
+    w->msw_2_kswr = calloc(i, sizeof(msw_kswr_v));
+    w->msw_2_xtra = calloc(i, sizeof(int_v));
 
     for (i = 0; i < opt->n_threads; ++i) {
         w->aux[i] = smem_aux_init();
@@ -1301,11 +1312,12 @@ mem_worker_t *init_mem_worker(const mem_opt_t *opt, const FMTIndex *fmt, const b
 		w->isize_arr[i] = calloc(4, sizeof(uint64_v));
         w->matesw_arr8[i] = calloc(1, sizeof(matesw_data_v));
 		w->matesw_arr16[i] = calloc(1, sizeof(matesw_data_v));
+        w->msw_2_arr8[i] = calloc(1, sizeof(matesw_data_t*));
         for (j = 0; j < opt->batch_size; ++j) {
-			kv_init(w->smem_arr[i][j].mem);
+            kv_init(w->smem_arr[i][j].mem);
 			kv_init(w->smem_arr[i][j].pos_arr);
 			kv_init(w->chain_arr[i][j]);
-		}
+        }
     }
     return w;
 }
diff --git a/bwamem.h b/bwamem.h
index fa9c0bd..8ab06d5 100644
--- a/bwamem.h
+++ b/bwamem.h
@@ -32,6 +32,7 @@
 #include "bwa.h"
 #include "fmt_idx.h"
 #include "utils.h"
+#include "ksw_align_avx.h"
 
 #define MEM_MAPQ_COEF 30.0
 #define MEM_MAPQ_MAX  60
@@ -84,6 +85,7 @@ typedef struct {
 	int max_matesw;         // perform maximally max_matesw rounds of mate-SW for each end
 	int max_XA_hits, max_XA_hits_alt; // if there are max_hits or fewer, output them all
 	int8_t mat[25];         // scoring matrix; mat[0] == 0 if unset
+    int msw_batch_size; // 每次处理的msw的个数，64的倍数
 } mem_opt_t;
 
 typedef struct {
@@ -166,8 +168,26 @@ typedef struct {
     int max_msw_ref_len; // mate sw里的最长的ref长度 
 } mem_stats_t;  // 记录一些用到的数据统计，比如最长ref长度，最长seq长度等
 
+typedef kvec_t(byte_v) byte_vv;
+
 typedef struct {
-	int calc_isize;
+    uint8_t* seq; // 只是指向seq的指针
+    int l_seq; // seq长度
+} msw_seq_t;
+
+typedef struct {
+    uint8_t* ref;
+    int l_ref;
+} msw_ref_t;
+
+typedef kvec_t(msw_seq_t) msw_seq_v;
+
+typedef kvec_t(msw_ref_t) msw_ref_v;
+
+typedef kvec_t(kswr_avx_t) msw_kswr_v;
+
+typedef struct {
+    int calc_isize;
 	const mem_opt_t *opt;
 	const bwt_t *bwt;
 	const FMTIndex *fmt;
@@ -182,13 +202,27 @@ typedef struct {
 	mem_alnreg_v *regs;
 	uint64_v **isize_arr;
     matesw_data_v** matesw_arr8; // 收集需要做mate sw的任务的数据
+    matesw_ptr_v** msw_2_arr8; // 第二阶段任务，每个线程单独收集
     matesw_data_v** matesw_arr16;
     //matesw_range_v msw_range_arr8;
     //matesw_range_v msw_range_arr16;
     matesw_ptr_v msw_tasks8; // 需要做mates sw的任务的指针
     matesw_ptr_v msw_tasks16;
-    matesw_buf_t* msw_buf; // mate sw过程用到的缓存空间
+    matesw_ptr_v msw_2_tasks8; // msw第二阶段任务
+    matesw_buf_t* msw_buf;   // mate sw过程用到的缓存空间
     mem_stats_t* mem_stats;  // 记录一些开辟空间等用到的统计数据，如seq长度，ref长度等
+
+    byte_vv* msw_ref; // 获取ref时的缓存空间，避免频繁的内存开辟和释放
+    msw_seq_v* msw_seq; // seq的缓存空间，个数由msw_batch_size决定，上面的ref一样
+    msw_kswr_v* msw_kswr; // 计算结果
+    int_v* msw_xtra; // msw额外参数
+
+	// msw第二阶段
+    msw_ref_v* msw_2_ref;
+	msw_seq_v* msw_2_seq;
+    msw_kswr_v* msw_2_kswr;
+    int_v* msw_2_xtra;
+
     int64_t n_processed;
     int64_t n; // regs元素个数，可动态扩展
 	int64_t n_reads;
diff --git a/paired_sam.c b/paired_sam.c
index 4999230..2f8014a 100644
--- a/paired_sam.c
+++ b/paired_sam.c
@@ -48,9 +48,9 @@ static int get_rid_range(const bntseq_t* bns, const uint8_t* pac, int64_t* beg,
 }
 
 // 获取ref序列
-static inline uint8_t* get_ref_seq(const bntseq_t* bns, const uint8_t* pac, int64_t beg, int64_t end) {
-    int64_t len;
-    return bns_get_seq(bns->l_pac, pac, beg, end, &len);
+static inline uint8_t* get_ref_seq(const bntseq_t* bns, const uint8_t* pac, int64_t beg, int64_t end, byte_v *buf) {
+    bns_get_seq_no_alloc(bns->l_pac, pac, beg, end, &buf->n, &buf->m, &buf->a);
+    return buf->a;
 }
 
 // 计算当前seq是否需要做matesw，需要的话保存必要的数据
@@ -111,105 +111,232 @@ static void get_matesw_data(const mem_opt_t* opt, const bntseq_t* bns, const uin
 static void worker_matesw_data(void* data, int idx, int tid) {
     mem_worker_t* w = (mem_worker_t*)data;
     const mem_opt_t* opt = w->opt;
-    int64_t i_s = idx << 1;
-    bseq1_t* s = &w->seqs[i_s];
-    mem_alnreg_v* a = &w->regs[i_s];
-    s->msw.n = 0; // 清空之前的matesw数据
-    int i, j;
-    mem_alnreg_v b[2];
-    kv_init(b[0]);
-    kv_init(b[1]);
-    // fprintf(stderr, "zzh test\n");
-    for (i = 0; i < 2; ++i)
-        for (j = 0; j < a[i].n; ++j)
-            if (a[i].a[j].score >= a[i].a[0].score - opt->pen_unpaired)
-                kv_push(mem_alnreg_t, b[i], a[i].a[j]);
-    for (i = 0; i < 2; ++i)
-        for (j = 0; j < b[i].n && j < opt->max_matesw; ++j)
-            get_matesw_data(opt, w->bns, w->pac, w->pes, &b[i].a[j], &s[!i], &a[!i], i_s + !i, &w->mem_stats[tid], w->matesw_arr8[tid], w->matesw_arr16[tid]);
-    free(b[0].a);
-    free(b[1].a);
+    int startIdx = idx * opt->batch_size;
+    int endIdx = (idx + 1) * opt->batch_size;
+    if (endIdx > w->n_reads)
+        endIdx = w->n_reads;
+
+    int id = 0;
+    for (id = startIdx; id < endIdx; id += 2) {
+        int64_t i_s = id;
+        bseq1_t* s = &w->seqs[i_s];
+        mem_alnreg_v* a = &w->regs[i_s];
+        s->msw.n = 0;  // 清空之前的matesw数据
+        int i, j;
+        mem_alnreg_v b[2];
+        kv_init(b[0]);
+        kv_init(b[1]);
+        // fprintf(stderr, "zzh test\n");
+        for (i = 0; i < 2; ++i)
+            for (j = 0; j < a[i].n; ++j)
+                if (a[i].a[j].score >= a[i].a[0].score - opt->pen_unpaired)
+                    kv_push(mem_alnreg_t, b[i], a[i].a[j]);
+        for (i = 0; i < 2; ++i)
+            for (j = 0; j < b[i].n && j < opt->max_matesw; ++j)
+                get_matesw_data(opt, w->bns, w->pac, w->pes, &b[i].a[j], &s[!i], &a[!i], i_s + !i, &w->mem_stats[tid], w->matesw_arr8[tid],
+                                w->matesw_arr16[tid]);
+        free(b[0].a);
+        free(b[1].a);
+    }
 }
 
 // 再多线程计算matesw，利用inter-query的simd并行
 static void worker_calc_matesw_avx512_u8(void* data, int idx, int tid) {
     mem_worker_t* w = (mem_worker_t*)data;
-    int startIdx = idx * SIMD512_WIDTH8;
-    int endIdx = (idx + 1) * SIMD512_WIDTH8;
+    const mem_opt_t* opt = w->opt;
+    int startIdx = idx * w->opt->msw_batch_size;
+    int endIdx = (idx + 1) * w->opt->msw_batch_size;
     if (endIdx > w->msw_tasks8.n)
         endIdx = w->msw_tasks8.n;
+    int roundEndIdx = ((endIdx + SIMD512_WIDTH8 - 1) / SIMD512_WIDTH8) * SIMD512_WIDTH8;
+
     int i = 0, j = 0, k = 0;
-    int maxSeqLen = 0, maxRefLen = 0;
-    uint8_t* refArr[SIMD512_WIDTH8] = {0};
-    uint8_t* seqArr[SIMD512_WIDTH8] = {0};
     int refLen[SIMD512_WIDTH8] = {0};
-    int seqLen[SIMD512_WIDTH8] = {0};
-    int seqQuantaLen[SIMD512_WIDTH8] = {0};
-    int xtraA[SIMD512_WIDTH8] = {0};
-    kswr_avx_t alns[SIMD512_WIDTH8] = {0};
 
     matesw_buf_t* msw_buf = &w->msw_buf[tid];  // 缓冲区
+    byte_vv* refs = &w->msw_ref[tid];
+    msw_seq_v* seqs = &w->msw_seq[tid];
+    int_v* xtras = &w->msw_xtra[tid];
+    msw_kswr_v* kswrs = &w->msw_kswr[tid];
 
+    // 获取ref和seq数据
+    PROF_START(msw_get_ref);
     for (i = startIdx, j = 0; i < endIdx; ++i, ++j) {
         matesw_data_t* task = kv_A(w->msw_tasks8, i);
         // 1. 获取对应的ref
-        refArr[j] = get_ref_seq(w->bns, w->pac, task->rb, task->re);
-        refLen[j] = task->re - task->rb;
-        maxRefLen = max_(maxRefLen, refLen[j]);
+        byte_v* ref = &kv_A(*refs, j);
+        get_ref_seq(w->bns, w->pac, task->rb, task->re, ref);
         // 2. 获取对应的seq
-        seqArr[j] = (uint8_t*)w->seqs[task->seq_id].seq;
-        seqLen[j] = w->seqs[task->seq_id].l_seq;
-        int quanta = (seqLen[j] + 16 - 1) / 16;  // based on SSE-8 bit lane
-        quanta *= 16;
-        seqQuantaLen[j] = quanta;
-        maxSeqLen = max_(maxSeqLen, quanta);
+        msw_seq_t* seq = &kv_A(*seqs, j);
+        seq->seq = (uint8_t*)w->seqs[task->seq_id].seq;
+        seq->l_seq = w->seqs[task->seq_id].l_seq;
         // 3. 其他数据
-        xtraA[j] = task->xtra;
+        xtras->a[j] = task->xtra;
     }
-    // 如果不到64个，补全剩下的
-    for (; j < SIMD512_WIDTH8; ++j) {
-        refArr[j] = refArr[0];
-        refLen[j] = refLen[0];
-        seqArr[j] = seqArr[0];
-        seqLen[j] = seqLen[0];
-        xtraA[j] = xtraA[0];
+    for (; i < roundEndIdx; ++i, ++j) {
+        byte_v* ref = &kv_A(*refs, j);
+        ref->n = 0;
+        msw_seq_t* seq = &kv_A(*seqs, j);
+        seq->l_seq = 0;
+        xtras->a[j] = 0;
     }
-    // 将ref和seq赋值给对应的用来计算的缓存
-    uint8_t* mySeq1SoA = msw_buf->refArr;
-    uint8_t* mySeq2SoA = msw_buf->seqArr;
+    PROF_END(tprof[T_MSW_GET_REF][tid], msw_get_ref);
 
-    for (j = 0; j < SIMD512_WIDTH8; ++j) {
-        uint8_t* seq1 = refArr[j];
-        uint8_t* seq2 = seqArr[j];
+    // 准备数据并进行计算
+    for (i = 0; i < endIdx - startIdx; i += SIMD512_WIDTH8) {
+        // 将ref和seq赋值给对应的用来计算的缓存
+        uint8_t* mySeq1SoA = msw_buf->refArr;
+        uint8_t* mySeq2SoA = msw_buf->seqArr;
+        int maxSeqLen = 0, maxRefLen = 0;
+
+        PROF_START(msw_pack_seq);
         // 处理ref
-        for (k = 0; k < refLen[j]; ++k) {
-            mySeq1SoA[k * SIMD512_WIDTH8 + j] = (seq1[k] == AMBIG_ ? AMBR : seq1[k]);
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            byte_v* ref = &refs->a[i + j];
+            uint8_t* seq1 = ref->a;
+            refLen[j] = ref->n;
+            // 处理ref
+            for (k = 0; k < ref->n; ++k) {
+                mySeq1SoA[k * SIMD512_WIDTH8 + j] = (seq1[k] == AMBIG_ ? AMBR : seq1[k]);
+            }
+            maxRefLen = max_(maxRefLen, ref->n);
         }
-        for (; k < maxRefLen; ++k) {
-            mySeq1SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            byte_v* ref = &refs->a[i + j];
+            for (k = ref->n; k < maxRefLen; ++k) {
+                mySeq1SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+            }
         }
-        // 处理seq
-        for (k = 0; k < seqLen[j]; ++k) {
-            mySeq2SoA[k * SIMD512_WIDTH8 + j] = (seq2[k] == AMBIG_ ? AMBR : seq2[k]);
+
+        // 处理query
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_seq_t* seq = &kv_A(*seqs, i + j);
+            uint8_t* seq2 = seq->seq;
+            int quanta = ((seq->l_seq + 16 - 1) / 16) * 16;  // based on SSE-8 bit lane
+            for (k = 0; k < seq->l_seq; k++) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = (seq2[k] == AMBIG_ ? AMBQ : seq2[k]);
+            }
+            for (; k < quanta; ++k) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = DUMMY5; // SSE quanta
+            }
+            maxSeqLen = max_(maxSeqLen, quanta);
         }
-        for (; k < seqQuantaLen[j]; ++k) {
-            mySeq2SoA[k * SIMD512_WIDTH8 + j] = DUMMY5;
-        }
-        for (; k < maxSeqLen; ++k) {
-            mySeq2SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_seq_t* seq = &kv_A(*seqs, i + j);
+            int quanta = ((seq->l_seq + 16 - 1) / 16) * 16;  // based on SSE-8 bit lane
+            for (k = quanta; k < maxSeqLen; k++) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+            }
         }
+        PROF_END(tprof[T_MSW_PACK_SEQ][tid], msw_pack_seq);
+
+        // 利用smid指令计算
+        PROF_START(msw_1);
+        ksw_align_avx512_u8(opt->a, -1 * opt->b, opt->o_ins, opt->e_ins, opt->o_del, opt->e_del, msw_buf, mySeq1SoA, mySeq2SoA, maxRefLen, maxSeqLen,
+                            &xtras->a[i], refLen, &kswrs->a[i], 0);
+        PROF_END(tprof[T_MSW_1][tid], msw_1);
     }
 
-    const mem_opt_t* opt = w->opt;
-    // 利用smid指令计算
-    ksw_align_avx512_u8(opt->a, -1 * opt->b, opt->o_ins, opt->e_ins, opt->o_del, opt->e_del, msw_buf, mySeq1SoA, mySeq2SoA, maxRefLen, maxSeqLen,
-                        xtraA, refLen, alns, 0);
-    // 保存结果
-    for (i = startIdx, j = 0; i < endIdx; ++i, ++j) {
+#if 1
+    msw_ref_v* refs2 = &w->msw_2_ref[tid];
+    msw_seq_v* seqs2 = &w->msw_2_seq[tid];
+    int_v* xtras2 = &w->msw_2_xtra[tid];
+    msw_kswr_v* kswrs2 = &w->msw_2_kswr[tid];
+
+    int_v msw_task_ids; // 用于第二阶段任务统计
+    kv_init(msw_task_ids);
+    // 保存结果，并筛选第二阶段msw计算的任务
+    for (i = startIdx, j = 0, k = 0; i < endIdx; ++i, ++j) {
         matesw_data_t* task = kv_A(w->msw_tasks8, i);
-        task->aln = alns[j];
-        free(refArr[j]);
+        task->aln = kswrs->a[j];
+        if ((task->xtra & KSW_XSTART) == 0 || ((task->xtra & KSW_XSUBO) && task->aln.score < (task->xtra & 0xffff)))
+            continue;
+        task->xtra = KSW_XSTOP | task->aln.score;
+        kv_push(int, msw_task_ids, j);
+
+        // 1. 获取对应的ref
+        byte_v* ref = &kv_A(*refs, j);
+        msw_ref_t* ref2 = &kv_A(*refs2, k);
+        ref2->l_ref = ref->n;
+        ref2->ref = ref->a;
+        // 2. 获取对应的seq
+        msw_seq_t* seq = &kv_A(*seqs, j);
+        msw_seq_t* seq2 = &kv_A(*seqs2, k);
+        seq2->seq = seq->seq;
+        seq2->l_seq = seq->l_seq;
+
+        // 3. 其他数据
+        xtras2->a[k] = task->xtra;
+        ++k;
     }
+    roundEndIdx = ((msw_task_ids.n + SIMD512_WIDTH8 - 1) / SIMD512_WIDTH8) * SIMD512_WIDTH8;
+    for (; k < roundEndIdx; ++k) {
+        msw_ref_t* ref2 = &kv_A(*refs2, k);
+        ref2->l_ref = 0;
+        msw_seq_t* seq2 = &kv_A(*seqs2, k);
+        seq2->l_seq = 0;
+        xtras2->a[k] = 0;
+    }
+
+    // 准备数据并进行计算
+    for (i = 0; i < msw_task_ids.n; i += SIMD512_WIDTH8) {
+        // 将ref和seq赋值给对应的用来计算的缓存
+        uint8_t* mySeq1SoA = msw_buf->refArr;
+        uint8_t* mySeq2SoA = msw_buf->seqArr;
+        int maxSeqLen = 0, maxRefLen = 0;
+
+        PROF_START(msw_pack_seq);
+        // 处理ref
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_ref_t* ref = &refs2->a[i + j];
+            uint8_t* seq1 = ref->ref;
+            refLen[j] = ref->l_ref;
+            // 处理ref
+            for (k = 0; k < ref->l_ref; ++k) {
+                mySeq1SoA[k * SIMD512_WIDTH8 + j] = (seq1[k] == AMBIG_ ? AMBR : seq1[k]);
+            }
+            maxRefLen = max_(maxRefLen, ref->l_ref);
+        }
+
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_ref_t* ref = &refs2->a[i + j];
+            for (k = ref->l_ref; k < maxRefLen; ++k) {
+                mySeq1SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+            }
+        }
+
+        // 处理query
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_seq_t* seq = &kv_A(*seqs2, i + j);
+            uint8_t* seq2 = seq->seq;
+            int quanta = ((seq->l_seq + 16 - 1) / 16) * 16;  // based on SSE-8 bit lane
+            for (k = 0; k < seq->l_seq; k++) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = (seq2[k] == AMBIG_ ? AMBQ : seq2[k]);
+            }
+            for (; k < quanta; ++k) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = DUMMY5;  // SSE quanta
+            }
+            maxSeqLen = max_(maxSeqLen, quanta);
+        }
+        for (j = 0; j < SIMD512_WIDTH8; ++j) {
+            msw_seq_t* seq = &kv_A(*seqs2, i + j);
+            int quanta = ((seq->l_seq + 16 - 1) / 16) * 16;  // based on SSE-8 bit lane
+            for (k = quanta; k < maxSeqLen; k++) {
+                mySeq2SoA[k * SIMD512_WIDTH8 + j] = 0xFF;
+            }
+        }
+        PROF_END(tprof[T_MSW_PACK_SEQ][tid], msw_pack_seq);
+
+        // 利用smid指令计算
+        PROF_START(msw_2);
+        ksw_align_avx512_u8(opt->a, -1 * opt->b, opt->o_ins, opt->e_ins, opt->o_del, opt->e_del, msw_buf, mySeq1SoA, mySeq2SoA, maxRefLen, maxSeqLen,
+                            &xtras2->a[i], refLen, &kswrs2->a[i], 1);
+        PROF_END(tprof[T_MSW_2][tid], msw_2);
+    }
+
+    kv_destroy(msw_task_ids);
+#endif
 }
 
 static void worker_calc_matesw16(void* data, int i, int tid) {}
@@ -249,8 +376,71 @@ static void update_mem_stats(mem_worker_t* w) {
 
 // 开辟缓冲区
 static void alloc_update_cache_avx512(mem_worker_t* w) {
-    int i = 0;
+    int i = 0, j = 0;
     for (i = 0; i < w->opt->n_threads; ++i) {
+        // 检查ref缓存空间
+        byte_vv* refs = &w->msw_ref[i];
+        if (refs->n < w->opt->msw_batch_size) {  // 初始化ref缓存
+            for (j = refs->n; j < w->opt->msw_batch_size; ++j) {
+                byte_v *p = kv_pushp(byte_v, *refs);
+                kv_init(*p);
+            }
+        }
+        msw_ref_v* refs2 = &w->msw_2_ref[i];
+        if (refs2->n < w->opt->msw_batch_size) {  // 初始化seq缓存
+            for (j = refs2->n; j < w->opt->msw_batch_size; ++j) {
+                msw_ref_t* p = kv_pushp(msw_ref_t, *refs2);
+                p->l_ref = 0;
+                p->ref = 0;
+            }
+        }
+
+        // 检查seq缓存空间
+        msw_seq_v* seqs = &w->msw_seq[i];
+        if (seqs->n < w->opt->msw_batch_size) {  // 初始化seq缓存
+            for (j = seqs->n; j < w->opt->msw_batch_size; ++j) {
+                msw_seq_t* p = kv_pushp(msw_seq_t, *seqs);
+                p->l_seq = 0;
+                p->seq = 0;
+            }
+        }
+        msw_seq_v* seqs2 = &w->msw_2_seq[i];
+        if (seqs2->n < w->opt->msw_batch_size) {  // 初始化seq缓存
+            for (j = seqs2->n; j < w->opt->msw_batch_size; ++j) {
+                msw_seq_t* p = kv_pushp(msw_seq_t, *seqs2);
+                p->l_seq = 0;
+                p->seq = 0;
+            }
+        }
+
+        msw_kswr_v* kswrs = &w->msw_kswr[i]; // 初始化msw结果
+        if (kswrs->n < w->opt->msw_batch_size) {
+            for (j = kswrs->n; j < w->opt->msw_batch_size; ++j) {
+                kv_pushp(kswr_avx_t, *kswrs);
+            }
+        }
+
+        msw_kswr_v* kswrs2 = &w->msw_2_kswr[i];  // 初始化msw结果
+        if (kswrs2->n < w->opt->msw_batch_size) {
+            for (j = kswrs2->n; j < w->opt->msw_batch_size; ++j) {
+                kv_pushp(kswr_avx_t, *kswrs2);
+            }
+        }
+
+        int_v* xtras = &w->msw_xtra[i];  // 初始化xtra
+        if (xtras->n < w->opt->msw_batch_size) {
+            for (j = xtras->n; j < w->opt->msw_batch_size; ++j) {
+                kv_pushp(int, *xtras);
+            }
+        }
+
+        int_v* xtras2 = &w->msw_2_xtra[i];  // 初始化xtra
+        if (xtras2->n < w->opt->msw_batch_size) {
+            for (j = xtras2->n; j < w->opt->msw_batch_size; ++j) {
+                kv_pushp(int, *xtras2);
+            }
+        }
+
         matesw_buf_t* b = &w->msw_buf[i];
         // 更新跟ref len有关的缓冲区
         if (b->ref_len < w->mem_stats[i].max_msw_ref_len) {
@@ -259,6 +449,11 @@ static void alloc_update_cache_avx512(mem_worker_t* w) {
             if (b->rowMax) _mm_free(b->rowMax);
             b->refArr = (uint8_t*)_mm_malloc(b->ref_len * SIMD512_WIDTH8 * sizeof(uint8_t), 64);
             b->rowMax = (uint8_t*)_mm_malloc(b->ref_len * SIMD512_WIDTH8 * sizeof(uint8_t), 64);
+            for (j = 0; j < w->opt->msw_batch_size; ++j) {
+                byte_v* ref = &kv_A(*refs, j);
+                kv_resize(uint8_t, *ref, b->ref_len);
+                // ref->n = ref->m;
+            }
         }
         // 更新跟seq len有关的缓冲区
         if (b->seq_len < w->mem_stats[i].max_seq_len) {
@@ -287,9 +482,10 @@ void gen_paired_sam(mem_worker_t* w) {
     if (w->opt->flag & MEM_F_NO_RESCUE) {
         kt_for(w->opt->n_threads, worker_sam, w, w->n_reads >> 1);  // generate alignment
     } else {
+        int batch_n = (w->n_reads + w->opt->batch_size - 1) / w->opt->batch_size;
         // 1. 计算哪些read需要matesw
         PROF_START(get_matesw_data);
-        kt_for(w->opt->n_threads, worker_matesw_data, w, w->n_reads >> 1);
+        kt_for(w->opt->n_threads, worker_matesw_data, w, batch_n);
         PROF_END(gprof[G_get_matesw_data], get_matesw_data);
         // 更新stats
         PROF_START(update_stats_cache);
@@ -303,10 +499,11 @@ void gen_paired_sam(mem_worker_t* w) {
         gather_matesw_task(w, w->matesw_arr16, &w->msw_tasks16);
         PROF_END(gprof[G_gather_matesw_task], gather_matesw_task);
         PROF_START(calc_matesw);
+        int msw_batch_n = (w->msw_tasks8.n + w->opt->msw_batch_size - 1) / w->opt->msw_batch_size;
         if (w->msw_tasks8.n > 0)
-            kt_for(w->opt->n_threads, worker_calc_matesw_avx512_u8, w, (w->msw_tasks8.n + SIMD512_WIDTH8 - 1) / SIMD512_WIDTH8);
+            kt_for(w->opt->n_threads, worker_calc_matesw_avx512_u8, w, msw_batch_n);
         if (w->msw_tasks16.n > 0)
-            kt_for(w->opt->n_threads, worker_calc_matesw16, w, (w->msw_tasks16.n + SIMD512_WIDTH16 - 1) / SIMD512_WIDTH16);
+            kt_for(w->opt->n_threads, worker_calc_matesw16, w, msw_batch_n);
         PROF_END(gprof[G_calc_matesw], calc_matesw);
         // 3. 
         kt_for(w->opt->n_threads, workder_gen_sam, w, w->n_reads >> 1);
diff --git a/profiling.c b/profiling.c
index 833d254..cf3efae 100644
--- a/profiling.c
+++ b/profiling.c
@@ -126,6 +126,20 @@ int display_stats(int nthreads)
     fprintf(stderr, "calc_matesw: %0.2lf s\n", gprof[G_calc_matesw] * 1.0 / proc_freq);
     fprintf(stderr, "gen_sam: %0.2lf s\n", gprof[G_gen_sam] * 1.0 / proc_freq);
 
+    find_opt(tprof[T_MSW_GET_REF], nthreads, &max, &min, &avg);
+    fprintf(stderr, "msw_get_ref:         %0.2lf s\n", avg);
+
+    find_opt(tprof[T_MSW_PACK_SEQ], nthreads, &max, &min, &avg);
+    fprintf(stderr, "msw_pack_seq:         %0.2lf s\n", avg);
+
+    find_opt(tprof[T_MSW_1], nthreads, &max, &min, &avg);
+    fprintf(stderr, "msw_1:         %0.2lf s\n", avg);
+
+    find_opt(tprof[T_MSW_2], nthreads, &max, &min, &avg);
+    fprintf(stderr, "msw_2:         %0.2lf s\n", avg);
+
+
+
     fprintf(stderr, "more num: %ld\n", more_num);
     fprintf(stderr, "no more num: %ld\n", not_more_num);
 
diff --git a/profiling.h b/profiling.h
index ffad5e2..88e1e1d 100644
--- a/profiling.h
+++ b/profiling.h
@@ -102,6 +102,10 @@ enum
     T_SEEDING,
     T_EXTENSION,
     T_SAM,
+    T_MSW_1,
+    T_MSW_2,
+    T_MSW_GET_REF,
+    T_MSW_PACK_SEQ,
 };
 
 int display_stats(int);
diff --git a/run.sh b/run.sh
index 756933b..1446e8a 100755
--- a/run.sh
+++ b/run.sh
@@ -2,8 +2,8 @@ thread=32
 
 make -j 16
 
-n1=~/data/dataset/D1/n1.fq.gz
-n2=~/data/dataset/D1/n2.fq.gz
+n1=~/data/dataset/D2/n1.fq.gz
+n2=~/data/dataset/D2/n2.fq.gz
 
 reference=~/data/reference/fmt/human_g1k_v37_decoy.fasta